Immunocytochemical Demonstration of Taurine

  • S. Madsen
  • O. P. Ottersen
  • J. Storm-Mathisen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 217)


The exact localization of a compound in normal tissue and its redistribution in neurological diseases and disease models can give important clues as to the functional role of the compound in question. In the case of taurine (2-aminoethanesulfonic acid), so far only biochemical methods have been available for its localization. Such methods will necessarily have a limited anatomical resolution and may easily fail to detect important pathological alterations in the cellular and subcellular distribution of taurine. A much better anatomical resolution is provided by the immunocytochemical technique, which has recently been adapted to amino acids (24). Antisera against conjugated amino acids have been employed to map the light- and electronmicroscopic localization of GABA, glutamate, glutamine, asparatate, and glycine in normal nerve tissue (1,2,6,9,10,17,18,19,22,24), and have also been used to study the redistribution of transmitter amino acids in experimental models of epilepsy (14).


Basket Cell Cysteic Acid Purkinje Cell Layer Taurine Concentration Conjugate Amino Acid 
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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • S. Madsen
    • 1
  • O. P. Ottersen
    • 1
  • J. Storm-Mathisen
    • 1
  1. 1.Anatomical InstituteUniversity of OsloOslo 1Norway

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