Immunocytochemical Demonstration of Taurine

  • S. Madsen
  • O. P. Ottersen
  • J. Storm-Mathisen
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 217)

Abstract

The exact localization of a compound in normal tissue and its redistribution in neurological diseases and disease models can give important clues as to the functional role of the compound in question. In the case of taurine (2-aminoethanesulfonic acid), so far only biochemical methods have been available for its localization. Such methods will necessarily have a limited anatomical resolution and may easily fail to detect important pathological alterations in the cellular and subcellular distribution of taurine. A much better anatomical resolution is provided by the immunocytochemical technique, which has recently been adapted to amino acids (24). Antisera against conjugated amino acids have been employed to map the light- and electronmicroscopic localization of GABA, glutamate, glutamine, asparatate, and glycine in normal nerve tissue (1,2,6,9,10,17,18,19,22,24), and have also been used to study the redistribution of transmitter amino acids in experimental models of epilepsy (14).

Keywords

Basket Cell Cysteic Acid Purkinje Cell Layer Taurine Concentration Conjugate Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Campistron, G., Buijs, R.M., and Geffard, M., 1986, Specific antibodies against aspartate and their immunocytochemical application in the rat brain. Brain Res., 365:179–184.PubMedCrossRefGoogle Scholar
  2. 2.
    Campistron, G., Buijs, R.M., and Geffard, M., 1986, Glycine neurons in the brain and spinal cord. Antibody production and immunocytochemical localization, Brain Res., 376:400–405.PubMedCrossRefGoogle Scholar
  3. 3.
    Campistron, G., Geffard, M., and Buijs, R.M., 1986, Immunological approach to the detection of taurine and immunocytochemical results, J. Neurochem., 46:862–868.PubMedCrossRefGoogle Scholar
  4. 4.
    Chan-Palay, V., Lin, C.-T., Palay, S., Yamamoto, M., and Wu, J.-Y., 1982, Taurine in the mammalian cerebellum: demonstration by autoradiography with [3H]taurine and immunocytochemistry with antibodies against the taurine-synthesizing enzyme cysteinesulfinic acid decarboxylase, Proc. Natl. Acad. Sci. USA, 79:2695–2699.PubMedCrossRefGoogle Scholar
  5. 5.
    Chan-Palay, V., Palay, S.L., and Wu, J.-Y., 1982, Saggital cerebellar microbands of taurine neurons: immunocytochemical demonstration by using antibodies against the taurine-synthesizing enzyme cysteinesulfinic acid decarboxylase, Proc. Natl. Acad. Sci. USA, 79:4221–4225.PubMedCrossRefGoogle Scholar
  6. 6.
    Dale, N., Ottersen, O.P., Roberts, A., and Storm-Mathisen, J., 1986, Inhibitory neurones of a motor pattern generator in Xenopus revealed by antibodies to glycine, Nature (London), 324:255–257.CrossRefGoogle Scholar
  7. 7.
    Hardy, P.M., Nicholls, A.C., and Rydon, H.N., 1976, The nature of the cross-linking of proteins by glutaraldehyde. I. Interactions of glutaraldehyde with the amino-groups of 6-aminohexanoic acid and of alpha-N-acetyl-lysine, J. Chem. Soc. Perkin Trans., 9:958–962.CrossRefGoogle Scholar
  8. 8.
    Hardy, P.M., Hughes, G.J., and Rydon, H.N., 1979, The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quarternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a crosslinking entity, J. Chem Soc. Perkin Trans., 1:2282–2288.CrossRefGoogle Scholar
  9. 9.
    Hodgson, A.J., Penke, B., Erdei, A., Chubb, I.W., and Somogyi, P., 1985, Antisera to gamma-aminobutyric acid. I. Production and characterization using a new model system, J. Histochem. Cytochem., 33:229–239.PubMedCrossRefGoogle Scholar
  10. 10.
    Laake, J.H., Gundersen, V., Nordbo, G., Ottersen, O.P., and Storm-Mathisen, J., 1986, An antiserum against glutamine, in: “Excitatory amino acids,” P.J. Roberts, J. Storm-Mathisen and H.F. Bradford, eds., Macmillan, London pp. 448–450.Google Scholar
  11. 11.
    Larsson, L.-I., 1983, Methods for immunocytochemistry of neurohormonal peptides, in: “Handbook of chemical neuroanatomy. Volume 1: Methods in chemical neuroanatomy,” A. Bjorklund and T. Hokfelt, eds., Elsevier, Amsterdam, pp. 147–209.Google Scholar
  12. 12.
    Madsen, S., Lehmann, A., Ottersen, O.P., and Storm-Mathisen, J., N-methyl-DL-aspartate (NMDA) induced redistribution of amino acids in the hippocampal formation visualized by immunocytochemistry, in: “Excitatory amino acids,” P.J. Roberts, J. Storm-Mathisen and H.F. Bradford, eds., Macmillan, London pp. 444–445.Google Scholar
  13. 13.
    Madsen, S., Ottersen, O.P., and Storm-Mathisen, J., 1985, Immunocytochemical visualization of taurine: neuronal localizaton in the rat cerebellum, Neurosci. Lett., 60:255–260.PubMedCrossRefGoogle Scholar
  14. 14.
    Meldrum, B.S., Swan, J.H., Ottersen, O.P., and Storm-Mathisen, J., 1986, Redistribution of transmitter amino acids in rat hippocampus and cerebellum during seizures induced by L-allylglycine and bicuculline: an immunocytochemical study with antisera against conjugated GABA, glutamate, and aspartate, Neuroscience, (in press).Google Scholar
  15. 15.
    Okamoto, K., Kimura H., and Sakai, Y., 1982, Evidence for taurine as an inhibitory neurotransmittor In cerebellar stellate interneurones: selective antagonism by TAG (6-aminomethyl-3-methyl-4H,1,2,4-benzo-thiadiazine-l,l-dioxide), Brain Res., 265:163–168.CrossRefGoogle Scholar
  16. 16.
    Ottersen, O.P., Madsen, S., Meldrum, B.S., and Storm-Mathisen, J., 1985, Taurine localization in the hippocampal formation of the senegalese baboon Papio Papio: an immunocytochemical study with an antiserum against conjugated taurine, Exp. Brain res., 59:457–462.PubMedCrossRefGoogle Scholar
  17. 17.
    Ottersen, O.P., and Storm-Mathisen, J., 1984, GABA-containing neurones in the thalamus and pretecturn of the rodent, Anat. Embryol., 170:197–207.PubMedCrossRefGoogle Scholar
  18. 18.
    Ottersen, O.P., and Storm-Mathisen, J., 1984, Glutamate-and GABA-containing neurones in the mouse and rat brain as demonstrated with a new immunocytochemical technique, J. Comp. Neurol., 229:374–392.PubMedCrossRefGoogle Scholar
  19. 19.
    Ottersen, O.P., and Storm-Mathisen, J., 1985, Different neuronal localization of aspartate-like and glutamate-like immunoreactivities in the hippocampus of rat, guinea-pig and Sengalese baboon (Papio papio), with a note on the distribution of gamma-aminobutyrate, Neuroscience, 16:589–606.PubMedCrossRefGoogle Scholar
  20. 20.
    Ottersen, O.P., Storm-Mathisen, J., Madsen, S., Skumlien, S., and Stromhaug, J., 1986, Evaluation of the immunocytochemical method for amino acids, Med. biol., 64:147–158.PubMedGoogle Scholar
  21. 21.
    Perry, T.L., Kish, S.J., and Hansen, S.H., 1979, Gamma-vinyl GABA: effects of chronic administration on the metabolism of GABA and other amino compounds, J. Neurochem., 32:1641–1645.PubMedCrossRefGoogle Scholar
  22. 22.
    Seguela, P., Geffard, M., Buijs, R.M., and Le Moal, M., 1984, Antibodies against gamma-aminobutyric acid: Specificity studies and immunocytochemical results, Proc. Natl. Acad. Sci. USA, 81:3888–3892.PubMedCrossRefGoogle Scholar
  23. 23.
    Sternberger, L.A., 1979, Immunocytochemistry, 2nd ed., John Wiley, New York, 354 pp.Google Scholar
  24. 24.
    Storm-Mathisen, J., Leknes, A.K., Bore, A.T., Vaaland, J.L., Edminson, P., Haug, F.-M.S., and Ottersen, O.P., 1983, First visualizaion of glutamate and GABA in neurones by immunocytochemistry, Nature (London), 301:517–520.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • S. Madsen
    • 1
  • O. P. Ottersen
    • 1
  • J. Storm-Mathisen
    • 1
  1. 1.Anatomical InstituteUniversity of OsloOslo 1Norway

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