Abstract
The exact localization of a compound in normal tissue and its redistribution in neurological diseases and disease models can give important clues as to the functional role of the compound in question. In the case of taurine (2-aminoethanesulfonic acid), so far only biochemical methods have been available for its localization. Such methods will necessarily have a limited anatomical resolution and may easily fail to detect important pathological alterations in the cellular and subcellular distribution of taurine. A much better anatomical resolution is provided by the immunocytochemical technique, which has recently been adapted to amino acids (24). Antisera against conjugated amino acids have been employed to map the light- and electronmicroscopic localization of GABA, glutamate, glutamine, asparatate, and glycine in normal nerve tissue (1,2,6,9,10,17,18,19,22,24), and have also been used to study the redistribution of transmitter amino acids in experimental models of epilepsy (14).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Campistron, G., Buijs, R.M., and Geffard, M., 1986, Specific antibodies against aspartate and their immunocytochemical application in the rat brain. Brain Res., 365:179–184.
Campistron, G., Buijs, R.M., and Geffard, M., 1986, Glycine neurons in the brain and spinal cord. Antibody production and immunocytochemical localization, Brain Res., 376:400–405.
Campistron, G., Geffard, M., and Buijs, R.M., 1986, Immunological approach to the detection of taurine and immunocytochemical results, J. Neurochem., 46:862–868.
Chan-Palay, V., Lin, C.-T., Palay, S., Yamamoto, M., and Wu, J.-Y., 1982, Taurine in the mammalian cerebellum: demonstration by autoradiography with [3H]taurine and immunocytochemistry with antibodies against the taurine-synthesizing enzyme cysteinesulfinic acid decarboxylase, Proc. Natl. Acad. Sci. USA, 79:2695–2699.
Chan-Palay, V., Palay, S.L., and Wu, J.-Y., 1982, Saggital cerebellar microbands of taurine neurons: immunocytochemical demonstration by using antibodies against the taurine-synthesizing enzyme cysteinesulfinic acid decarboxylase, Proc. Natl. Acad. Sci. USA, 79:4221–4225.
Dale, N., Ottersen, O.P., Roberts, A., and Storm-Mathisen, J., 1986, Inhibitory neurones of a motor pattern generator in Xenopus revealed by antibodies to glycine, Nature (London), 324:255–257.
Hardy, P.M., Nicholls, A.C., and Rydon, H.N., 1976, The nature of the cross-linking of proteins by glutaraldehyde. I. Interactions of glutaraldehyde with the amino-groups of 6-aminohexanoic acid and of alpha-N-acetyl-lysine, J. Chem. Soc. Perkin Trans., 9:958–962.
Hardy, P.M., Hughes, G.J., and Rydon, H.N., 1979, The nature of the cross-linking of proteins by glutaraldehyde. Part 2. The formation of quarternary pyridinium compounds by the action of glutaraldehyde on proteins and the identification of a 3-(2-piperidyl)-pyridinium derivative, anabilysine, as a crosslinking entity, J. Chem Soc. Perkin Trans., 1:2282–2288.
Hodgson, A.J., Penke, B., Erdei, A., Chubb, I.W., and Somogyi, P., 1985, Antisera to gamma-aminobutyric acid. I. Production and characterization using a new model system, J. Histochem. Cytochem., 33:229–239.
Laake, J.H., Gundersen, V., Nordbo, G., Ottersen, O.P., and Storm-Mathisen, J., 1986, An antiserum against glutamine, in: “Excitatory amino acids,” P.J. Roberts, J. Storm-Mathisen and H.F. Bradford, eds., Macmillan, London pp. 448–450.
Larsson, L.-I., 1983, Methods for immunocytochemistry of neurohormonal peptides, in: “Handbook of chemical neuroanatomy. Volume 1: Methods in chemical neuroanatomy,” A. Bjorklund and T. Hokfelt, eds., Elsevier, Amsterdam, pp. 147–209.
Madsen, S., Lehmann, A., Ottersen, O.P., and Storm-Mathisen, J., N-methyl-DL-aspartate (NMDA) induced redistribution of amino acids in the hippocampal formation visualized by immunocytochemistry, in: “Excitatory amino acids,” P.J. Roberts, J. Storm-Mathisen and H.F. Bradford, eds., Macmillan, London pp. 444–445.
Madsen, S., Ottersen, O.P., and Storm-Mathisen, J., 1985, Immunocytochemical visualization of taurine: neuronal localizaton in the rat cerebellum, Neurosci. Lett., 60:255–260.
Meldrum, B.S., Swan, J.H., Ottersen, O.P., and Storm-Mathisen, J., 1986, Redistribution of transmitter amino acids in rat hippocampus and cerebellum during seizures induced by L-allylglycine and bicuculline: an immunocytochemical study with antisera against conjugated GABA, glutamate, and aspartate, Neuroscience, (in press).
Okamoto, K., Kimura H., and Sakai, Y., 1982, Evidence for taurine as an inhibitory neurotransmittor In cerebellar stellate interneurones: selective antagonism by TAG (6-aminomethyl-3-methyl-4H,1,2,4-benzo-thiadiazine-l,l-dioxide), Brain Res., 265:163–168.
Ottersen, O.P., Madsen, S., Meldrum, B.S., and Storm-Mathisen, J., 1985, Taurine localization in the hippocampal formation of the senegalese baboon Papio Papio: an immunocytochemical study with an antiserum against conjugated taurine, Exp. Brain res., 59:457–462.
Ottersen, O.P., and Storm-Mathisen, J., 1984, GABA-containing neurones in the thalamus and pretecturn of the rodent, Anat. Embryol., 170:197–207.
Ottersen, O.P., and Storm-Mathisen, J., 1984, Glutamate-and GABA-containing neurones in the mouse and rat brain as demonstrated with a new immunocytochemical technique, J. Comp. Neurol., 229:374–392.
Ottersen, O.P., and Storm-Mathisen, J., 1985, Different neuronal localization of aspartate-like and glutamate-like immunoreactivities in the hippocampus of rat, guinea-pig and Sengalese baboon (Papio papio), with a note on the distribution of gamma-aminobutyrate, Neuroscience, 16:589–606.
Ottersen, O.P., Storm-Mathisen, J., Madsen, S., Skumlien, S., and Stromhaug, J., 1986, Evaluation of the immunocytochemical method for amino acids, Med. biol., 64:147–158.
Perry, T.L., Kish, S.J., and Hansen, S.H., 1979, Gamma-vinyl GABA: effects of chronic administration on the metabolism of GABA and other amino compounds, J. Neurochem., 32:1641–1645.
Seguela, P., Geffard, M., Buijs, R.M., and Le Moal, M., 1984, Antibodies against gamma-aminobutyric acid: Specificity studies and immunocytochemical results, Proc. Natl. Acad. Sci. USA, 81:3888–3892.
Sternberger, L.A., 1979, Immunocytochemistry, 2nd ed., John Wiley, New York, 354 pp.
Storm-Mathisen, J., Leknes, A.K., Bore, A.T., Vaaland, J.L., Edminson, P., Haug, F.-M.S., and Ottersen, O.P., 1983, First visualizaion of glutamate and GABA in neurones by immunocytochemistry, Nature (London), 301:517–520.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Madsen, S., Ottersen, O.P., Storm-Mathisen, J. (1987). Immunocytochemical Demonstration of Taurine. In: Huxtable, R.J., Franconi, F., Giotti, A. (eds) The Biology of Taurine. Advances in Experimental Medicine and Biology, vol 217. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0405-8_29
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0405-8_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0407-2
Online ISBN: 978-1-4899-0405-8
eBook Packages: Springer Book Archive