Somatic Embryogenesis for Mass Cloning of Crop Plants

  • Joseph D. Lutz
  • James R. Wong
  • Jan Rowe
  • David M. Tricoli
  • Robert H. LawrenceJr.
Part of the Basic Life Sciences book series (BLSC, volume 32)


The large-scale cloning of crop plants potentially has broad applications in agriculture (23, 28, 45). The possibility of producing large numbers of plants of a single genotype evokes visions of applications to breeding programs, new seed production methods, and even an alternative to normal crop production from seeds. Over the last 10 to 15 years tissue culture techniques have found wide use in the commercial propagation of horticultural plants, particularly ornamentals, and in the elimination of specific pathogens for the production of pathogen-free plants. There has been little commercial use of tissue culture in the production of agricultural crops. The methods commonly used for the production of horticultural propagules are relatively labor-intensive, low volume, and have high unit costs compared with current agricultural seed practices. Highly mechanized culture systems able to efficiently produce large numbers of propagules must be developed if high-frequency cloning is to be a viable concept.


Somatic Embryo Somatic Embryogenesis Suspension Culture Zygotic Embryo Adventitious Shoot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Anderson, W.C., G.W. Meagher, and A.G. Nelson (1977) Cost of propagating broccoli plants through tissue culture. Hort. Sci. 12:543–544.Google Scholar
  2. 2.
    Ammirato, P.V. (1974) The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum carvi). Bot. Gaz. 135:328–337.CrossRefGoogle Scholar
  3. 3.
    Ammirato, P.V. (1977) Hormonal control of somatic embryo development from cultured cells of caraway. Plant Physiol. 59:579–586.PubMedCrossRefGoogle Scholar
  4. 4.
    Ammirato, P.V. (1983) Embryogenesis. In Handbook of Plant Cell Culture Vol. I: Techniques for Propagation and Breeding, D.A. Evans, W.R. Sharp, P.V. Ammirato, and Y. Yamada, eds. MacMillan Publ. Co., New York, pp. 82–123.Google Scholar
  5. 5.
    Ammirato, P.V. (1983) The regulation of somatic embryo development in plant cell cultures: Suspension culture techniques and hormone requirements. Bio/technology 3:68–74.CrossRefGoogle Scholar
  6. 6.
    Boxus, Ph., M. Quoirin, and J.M. Laine (1977) Large scale propagation of strawberry plants from tissue culture. In Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture, J. Reinert and Y.P.S. Bajaj, eds. Springer-Verlag, Berlin, Heidelberg, New York, pp. 130–143.Google Scholar
  7. 7.
    Conger, B.V., ed. (1981) Cloning Agricultural Plants via in vitro Techniques, CRC Press, Inc., Boca Raton, Florida.Google Scholar
  8. 8.
    Cooke, R.C. (1979) Homogenization as an aid in tissue culture propagation of Platycerium and Davallia. Hort. Sci. 14:21–22.Google Scholar
  9. 9.
    Currah, I.E. (1977) Fluid drilling research. National Vegetable Research Station Report, Wellesbourne, Warwick, England.Google Scholar
  10. 10.
    Currah, I.E., D. Gray, and T.H. Thomas (1973) The sowing of germinated vegetable seeds using a fluid drill. Ann. Appl. Biol. 76:311–318.CrossRefGoogle Scholar
  11. 11.
    Damiano, C. (1980) Strawberry micropropagation. In Proceedings of the Conference on Nursery Production of Fruit Plants Through Tissue Culture: Applications and Feasibility, Agricultural Research Science and Education Administration, USDA, Beltsville, Md., pp. 11–22.Google Scholar
  12. 12.
    Drew, R.K.L. (1979) The development of carrot (Daucus carota L.) embryoids (derived from cell suspension culture) into plantlets on a sugar-free basal medium. Hort. Res. 19:79–84.Google Scholar
  13. 13.
    Earle, E.D., and Y. Demarly, eds. (1982) Variability in Plants Regenerated from Tissue Culture, Praeger Publishers, New York.Google Scholar
  14. 14.
    Fujimura, T., and A. Konamine (1979) Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol. 64:162–164.PubMedCrossRefGoogle Scholar
  15. 15.
    Gengenbach, B.G., C.E. Green, and C. Donovan (1977) Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Natl. Acad. Sci. USA 74:5113–5117.PubMedCrossRefGoogle Scholar
  16. 16.
    Gray, D. (1981) Fluid drilling of vegetable seeds. Hort. Rev. 3:1–27.Google Scholar
  17. 17.
    Halperin, W., and D.F. Wetherell (1964) Adventive embryony in tissue cultures of the wild carrot, Daucus carota. Amer. J. Bot. 51:274–283.CrossRefGoogle Scholar
  18. 18.
    Halperin, W., and D.F. Wetherell (1965) Ontogeny of adventive embryos of wild carrot. Science 147:756–758.PubMedCrossRefGoogle Scholar
  19. 19.
    Jones, L.H. (1974) Factors influencing embryogenesis in carrot cultures Daucus carota L. Ann. Bot. 38:1077–1088.Google Scholar
  20. 20.
    Kamada, H., and H. Harada (1981) Changes in the endogenous level and effects of abscisic acid during somatic embryogenesis of Daucus carota L. Plant and Cell Physiol. 22:1423–1429.Google Scholar
  21. 21.
    Krikorian, A.D. (1982) Cloning higher plants from aseptically cultured tissues and cells. Biol. Rev. 57:151–218.CrossRefGoogle Scholar
  22. 22.
    Larkin, P.J., and W.R. Scowcroft (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197–214.CrossRefGoogle Scholar
  23. 23.
    Lawrence, Jr., R.H. (1981) In vitro plant cloning systems. Environ. Expt. Bot. 21:289–300.CrossRefGoogle Scholar
  24. 24.
    Lundergan, C., and J. Janick (1979) Low temperature storage of in vitro apple shoots (Malus domestica cv. Golden Delicious). Hort. Sci. 14:514–519.Google Scholar
  25. 25.
    McWilliam, A.A., S.W. Smith, and H.E. Street (1974) The origin and development of embryoids in suspension cultures of carrot (Daucus carota). Ann. Bot. 38:243–250.Google Scholar
  26. 26.
    Meins, Jr., F. (1982) The nature of cellular, heritable change in cytokinin habituation. In Variability in Plants Regenerated from Tissue Culture, E.D. Earle and Y. Demarly, eds. Praeger Press, New York, pp. 202–210.Google Scholar
  27. 27.
    Mullin, R.H., and D.E. Schlegel (1976) Cold storage maintenance of strawberry meristem plantlets. Hort. Sci. 11:100–107.Google Scholar
  28. 28.
    Murashige, T. (1974) Plant propagation through tissue cultures. In Annual Review of Plant Physiology, Vol. 25, W.R. Briggs, ed. Annual Reviews, Palo Alto, pp. 135–166.Google Scholar
  29. 29.
    Murashige, T. (1978) The impact of plant tissue culture on agriculture. In Frontiers of Plant Tissue Culture 1978, T.A. Thorpe, ed. The International Association for Plant Tissue Culture, Calgary, pp. 15–26.Google Scholar
  30. 30.
    Murashige, T. (1978) Principles of rapid propagation. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 14–24.Google Scholar
  31. 31.
    Nitzsche, W. (1983) Germplasm preservation. In Handbook of Plant Cell Culture. Vol. I: Techniques for Propagation and Breeding, D.A. Evans, W.R. Sharp, P.V. Ammirato, and Y. Yamada, eds. MacMillan Publishing Company, New York, pp. 782–805.Google Scholar
  32. 32.
    Oblesby, R.P. (1978) Tissue cultures of ornamentals and flowers: Problems and perspectives. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 59–61.Google Scholar
  33. 33.
    Redenbaugh, K., J. Nichol, M.E. Kossler, and B. Paasch (1984) Encapsulation of somatic embryos for artificial seed production. In Vitro 20:256.Google Scholar
  34. 34.
    Reinert, J. (1958) Untersuchungen uber die Morphogenese an Gewebekulturen. Ber. Dtsch. Bot. Ges. 71:15.Google Scholar
  35. 35.
    Reinert, J. (1959) Uber die Kontrolle der Morphogenese und die Induktion von Adventureembryonen an Gewebekulturen aus Karotten. Planta 53:318–333.CrossRefGoogle Scholar
  36. 36.
    Scowcroft, W.R. (1977) Somatic cell genetics and plant improvement. Adv. Agron. 29:39–81.CrossRefGoogle Scholar
  37. 37.
    Sharp, W.R., M.R. Sondahl, L. Caldas, and S.B. Maraffa (1980) The physiology of in vitro asexual embryogenesis. In Horticultural Reviews, Vol. 2, J. Janick, ed. AVI, Westport, Connecticut, pp. 268–310.Google Scholar
  38. 38.
    Skirvin, R.M. (1978) Natural and induced variation in tissue cultures. Euphytica 27:241–266.CrossRefGoogle Scholar
  39. 39.
    Sharp, W.R., P.O. Larsen, E.F. Paddock, and V. Raghaven, eds. (1979) Plant Cell and Tissue Culture: Principles and Applications, Ohio State University Press, Columbus, Ohio, 892 pp.Google Scholar
  40. 40.
    Smith, S.M., and H.E. Street (1974) The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann. Bot. 38:223–241.Google Scholar
  41. 41.
    Steward, F.C. (1958) Interpretations of the growth from free cells to carrot plants. Amer. J. Bot. 45:709–713.CrossRefGoogle Scholar
  42. 42.
    Street, H.E., and L.A. Withers (1974) The anatomy of embryogenesis in culture. In Tissue Culture and Plant Science, H.E. Street, ed. Academic Press, London, pp. 71–100.Google Scholar
  43. 43.
    Syono, K. (1965) Changes in organ forming capacity of carrot root calluses during subculture. Plant and Cell Physiol. 6:403–419.Google Scholar
  44. 44.
    Tisserat, B., E.B. Esan, and T. Murashige (1979) Somatic embryogenesis in angiosperms. In Horticultural Reviews, Vol. I, J. Janick, ed. AVI, Westport, Connecticut, pp. 1–78.Google Scholar
  45. 45.
    Walker, K.A., M.L. Wendeln, and E.G. Jaworski (1979) Organogenesis in callus tissue of Medicago sativa. The temporal separation of induction processes from differentiation processes. Plant Sci. Lett. 16:23–30.CrossRefGoogle Scholar
  46. 46.
    Warren, G.S., and M.W. Fowler (1977) A physical method for the separation of various stages in the embryogenesis of carrot cell cultures. Plant Sci. Lett. 9:71–76.CrossRefGoogle Scholar
  47. 47.
    Wetherell, D.F. (1978) In vitro embryoid formation in cells derived from somatic plant tissues. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 102–124.Google Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Joseph D. Lutz
    • 1
  • James R. Wong
    • 1
  • Jan Rowe
    • 1
  • David M. Tricoli
    • 1
    • 2
  • Robert H. LawrenceJr.
    • 1
  1. 1.Applied Genetics LaboratoryAgrigenetics CorporationBoulderUSA
  2. 2.Monsanto CompanySt. LouisUSA

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