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Somatic Embryogenesis for Mass Cloning of Crop Plants

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Tissue Culture in Forestry and Agriculture

Part of the book series: Basic Life Sciences ((BLSC,volume 32))

Abstract

The large-scale cloning of crop plants potentially has broad applications in agriculture (23, 28, 45). The possibility of producing large numbers of plants of a single genotype evokes visions of applications to breeding programs, new seed production methods, and even an alternative to normal crop production from seeds. Over the last 10 to 15 years tissue culture techniques have found wide use in the commercial propagation of horticultural plants, particularly ornamentals, and in the elimination of specific pathogens for the production of pathogen-free plants. There has been little commercial use of tissue culture in the production of agricultural crops. The methods commonly used for the production of horticultural propagules are relatively labor-intensive, low volume, and have high unit costs compared with current agricultural seed practices. Highly mechanized culture systems able to efficiently produce large numbers of propagules must be developed if high-frequency cloning is to be a viable concept.

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References

  1. Anderson, W.C., G.W. Meagher, and A.G. Nelson (1977) Cost of propagating broccoli plants through tissue culture. Hort. Sci. 12:543–544.

    Google Scholar 

  2. Ammirato, P.V. (1974) The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum carvi). Bot. Gaz. 135:328–337.

    Article  CAS  Google Scholar 

  3. Ammirato, P.V. (1977) Hormonal control of somatic embryo development from cultured cells of caraway. Plant Physiol. 59:579–586.

    Article  PubMed  CAS  Google Scholar 

  4. Ammirato, P.V. (1983) Embryogenesis. In Handbook of Plant Cell Culture Vol. I: Techniques for Propagation and Breeding, D.A. Evans, W.R. Sharp, P.V. Ammirato, and Y. Yamada, eds. MacMillan Publ. Co., New York, pp. 82–123.

    Google Scholar 

  5. Ammirato, P.V. (1983) The regulation of somatic embryo development in plant cell cultures: Suspension culture techniques and hormone requirements. Bio/technology 3:68–74.

    Article  Google Scholar 

  6. Boxus, Ph., M. Quoirin, and J.M. Laine (1977) Large scale propagation of strawberry plants from tissue culture. In Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture, J. Reinert and Y.P.S. Bajaj, eds. Springer-Verlag, Berlin, Heidelberg, New York, pp. 130–143.

    Google Scholar 

  7. Conger, B.V., ed. (1981) Cloning Agricultural Plants via in vitro Techniques, CRC Press, Inc., Boca Raton, Florida.

    Google Scholar 

  8. Cooke, R.C. (1979) Homogenization as an aid in tissue culture propagation of Platycerium and Davallia. Hort. Sci. 14:21–22.

    Google Scholar 

  9. Currah, I.E. (1977) Fluid drilling research. National Vegetable Research Station Report, Wellesbourne, Warwick, England.

    Google Scholar 

  10. Currah, I.E., D. Gray, and T.H. Thomas (1973) The sowing of germinated vegetable seeds using a fluid drill. Ann. Appl. Biol. 76:311–318.

    Article  Google Scholar 

  11. Damiano, C. (1980) Strawberry micropropagation. In Proceedings of the Conference on Nursery Production of Fruit Plants Through Tissue Culture: Applications and Feasibility, Agricultural Research Science and Education Administration, USDA, Beltsville, Md., pp. 11–22.

    Google Scholar 

  12. Drew, R.K.L. (1979) The development of carrot (Daucus carota L.) embryoids (derived from cell suspension culture) into plantlets on a sugar-free basal medium. Hort. Res. 19:79–84.

    Google Scholar 

  13. Earle, E.D., and Y. Demarly, eds. (1982) Variability in Plants Regenerated from Tissue Culture, Praeger Publishers, New York.

    Google Scholar 

  14. Fujimura, T., and A. Konamine (1979) Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol. 64:162–164.

    Article  PubMed  CAS  Google Scholar 

  15. Gengenbach, B.G., C.E. Green, and C. Donovan (1977) Inheritance of selected pathotoxin resistance in maize plants regenerated from cell cultures. Proc. Natl. Acad. Sci. USA 74:5113–5117.

    Article  PubMed  CAS  Google Scholar 

  16. Gray, D. (1981) Fluid drilling of vegetable seeds. Hort. Rev. 3:1–27.

    CAS  Google Scholar 

  17. Halperin, W., and D.F. Wetherell (1964) Adventive embryony in tissue cultures of the wild carrot, Daucus carota. Amer. J. Bot. 51:274–283.

    Article  CAS  Google Scholar 

  18. Halperin, W., and D.F. Wetherell (1965) Ontogeny of adventive embryos of wild carrot. Science 147:756–758.

    Article  PubMed  CAS  Google Scholar 

  19. Jones, L.H. (1974) Factors influencing embryogenesis in carrot cultures Daucus carota L. Ann. Bot. 38:1077–1088.

    CAS  Google Scholar 

  20. Kamada, H., and H. Harada (1981) Changes in the endogenous level and effects of abscisic acid during somatic embryogenesis of Daucus carota L. Plant and Cell Physiol. 22:1423–1429.

    CAS  Google Scholar 

  21. Krikorian, A.D. (1982) Cloning higher plants from aseptically cultured tissues and cells. Biol. Rev. 57:151–218.

    Article  Google Scholar 

  22. Larkin, P.J., and W.R. Scowcroft (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197–214.

    Article  Google Scholar 

  23. Lawrence, Jr., R.H. (1981) In vitro plant cloning systems. Environ. Expt. Bot. 21:289–300.

    Article  Google Scholar 

  24. Lundergan, C., and J. Janick (1979) Low temperature storage of in vitro apple shoots (Malus domestica cv. Golden Delicious). Hort. Sci. 14:514–519.

    Google Scholar 

  25. McWilliam, A.A., S.W. Smith, and H.E. Street (1974) The origin and development of embryoids in suspension cultures of carrot (Daucus carota). Ann. Bot. 38:243–250.

    Google Scholar 

  26. Meins, Jr., F. (1982) The nature of cellular, heritable change in cytokinin habituation. In Variability in Plants Regenerated from Tissue Culture, E.D. Earle and Y. Demarly, eds. Praeger Press, New York, pp. 202–210.

    Google Scholar 

  27. Mullin, R.H., and D.E. Schlegel (1976) Cold storage maintenance of strawberry meristem plantlets. Hort. Sci. 11:100–107.

    Google Scholar 

  28. Murashige, T. (1974) Plant propagation through tissue cultures. In Annual Review of Plant Physiology, Vol. 25, W.R. Briggs, ed. Annual Reviews, Palo Alto, pp. 135–166.

    Google Scholar 

  29. Murashige, T. (1978) The impact of plant tissue culture on agriculture. In Frontiers of Plant Tissue Culture 1978, T.A. Thorpe, ed. The International Association for Plant Tissue Culture, Calgary, pp. 15–26.

    Google Scholar 

  30. Murashige, T. (1978) Principles of rapid propagation. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 14–24.

    Google Scholar 

  31. Nitzsche, W. (1983) Germplasm preservation. In Handbook of Plant Cell Culture. Vol. I: Techniques for Propagation and Breeding, D.A. Evans, W.R. Sharp, P.V. Ammirato, and Y. Yamada, eds. MacMillan Publishing Company, New York, pp. 782–805.

    Google Scholar 

  32. Oblesby, R.P. (1978) Tissue cultures of ornamentals and flowers: Problems and perspectives. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 59–61.

    Google Scholar 

  33. Redenbaugh, K., J. Nichol, M.E. Kossler, and B. Paasch (1984) Encapsulation of somatic embryos for artificial seed production. In Vitro 20:256.

    Google Scholar 

  34. Reinert, J. (1958) Untersuchungen uber die Morphogenese an Gewebekulturen. Ber. Dtsch. Bot. Ges. 71:15.

    Google Scholar 

  35. Reinert, J. (1959) Uber die Kontrolle der Morphogenese und die Induktion von Adventureembryonen an Gewebekulturen aus Karotten. Planta 53:318–333.

    Article  Google Scholar 

  36. Scowcroft, W.R. (1977) Somatic cell genetics and plant improvement. Adv. Agron. 29:39–81.

    Article  CAS  Google Scholar 

  37. Sharp, W.R., M.R. Sondahl, L. Caldas, and S.B. Maraffa (1980) The physiology of in vitro asexual embryogenesis. In Horticultural Reviews, Vol. 2, J. Janick, ed. AVI, Westport, Connecticut, pp. 268–310.

    Google Scholar 

  38. Skirvin, R.M. (1978) Natural and induced variation in tissue cultures. Euphytica 27:241–266.

    Article  Google Scholar 

  39. Sharp, W.R., P.O. Larsen, E.F. Paddock, and V. Raghaven, eds. (1979) Plant Cell and Tissue Culture: Principles and Applications, Ohio State University Press, Columbus, Ohio, 892 pp.

    Google Scholar 

  40. Smith, S.M., and H.E. Street (1974) The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann. Bot. 38:223–241.

    Google Scholar 

  41. Steward, F.C. (1958) Interpretations of the growth from free cells to carrot plants. Amer. J. Bot. 45:709–713.

    Article  Google Scholar 

  42. Street, H.E., and L.A. Withers (1974) The anatomy of embryogenesis in culture. In Tissue Culture and Plant Science, H.E. Street, ed. Academic Press, London, pp. 71–100.

    Google Scholar 

  43. Syono, K. (1965) Changes in organ forming capacity of carrot root calluses during subculture. Plant and Cell Physiol. 6:403–419.

    CAS  Google Scholar 

  44. Tisserat, B., E.B. Esan, and T. Murashige (1979) Somatic embryogenesis in angiosperms. In Horticultural Reviews, Vol. I, J. Janick, ed. AVI, Westport, Connecticut, pp. 1–78.

    Google Scholar 

  45. Walker, K.A., M.L. Wendeln, and E.G. Jaworski (1979) Organogenesis in callus tissue of Medicago sativa. The temporal separation of induction processes from differentiation processes. Plant Sci. Lett. 16:23–30.

    Article  CAS  Google Scholar 

  46. Warren, G.S., and M.W. Fowler (1977) A physical method for the separation of various stages in the embryogenesis of carrot cell cultures. Plant Sci. Lett. 9:71–76.

    Article  Google Scholar 

  47. Wetherell, D.F. (1978) In vitro embryoid formation in cells derived from somatic plant tissues. In Propagation of Higher Plants Through Tissue Culture, A Bridge Between Research and Application, K.W. Hughes, R. Henke, and M. Constantin, eds. Technical Information Center, U.S. Dept. of Energy, Oak Ridge, pp. 102–124.

    Google Scholar 

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© 1985 Springer Science+Business Media New York

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Lutz, J.D., Wong, J.R., Rowe, J., Tricoli, D.M., Lawrence, R.H. (1985). Somatic Embryogenesis for Mass Cloning of Crop Plants. In: Henke, R.R., Hughes, K.W., Constantin, M.J., Hollaender, A., Wilson, C.M. (eds) Tissue Culture in Forestry and Agriculture. Basic Life Sciences, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0378-5_8

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  • DOI: https://doi.org/10.1007/978-1-4899-0378-5_8

  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4899-0378-5

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