Abstract
The availability of a protoplast regeneration system together with the new emerging techniques for genetic manipulation offer plant breeding specific advantages for developing new crop cultivars. The techniques for producing somatic hybrids, cybrids, and plant mutants require the ability to isolate large numbers of viable protoplasts capable of division and plant regeneration. Protoplast culture techniques are relatively new and there are still numerous obstacles to overcome before they are fully applicable to major horticultural and agronomic crops. Plants have been obtained from protoplasts in a number of crop species (1), but in many instances their yield and reliability have not been satisfactory for genetic manipulation.
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Shahin, E.A., Yashar, M. (1985). Factors Influencing Tomato Protoplast Development. In: Henke, R.R., Hughes, K.W., Constantin, M.J., Hollaender, A., Wilson, C.M. (eds) Tissue Culture in Forestry and Agriculture. Basic Life Sciences, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0378-5_6
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DOI: https://doi.org/10.1007/978-1-4899-0378-5_6
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