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In Vitro Callus Formation and Propagation of Sugarcane

  • M. Sarwar
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Cultures were established using the fully developed, top 4 buds from field-grown canes of Saccharum officinarum var. BL 4. During the initial culture, expiant browning was controlled by presoaking 30- to 50-mm stem sets, having one bud each, for 4 to 5 hr in 5% Clorox. During subculture, the death of in vitro-produced plantlets because of browning was controlled by keeping the cultures in the dark for the first week. Maximum bud proliferation was obtained on the media having 1 to 5 μM benzylaminopurine or 20 to 40 μM kinetin. Up to 40 plantlets were obtained in 50 days. Only one culture of explanted stem pith produced callus on 1.5 Ü 10−5 M 2,4-dichlorophenoxyacetic acid medium.

Keywords

Cell Culture Tissue Culture Acid Medium Agricultural Research Callus Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • M. Sarwar
    • 1
  1. 1.Tissue Culture LaboratoryPakistan Agricultural Research CouncilIslamabadPakistan

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