Abstract
Cultures were established using the fully developed, top 4 buds from field-grown canes of Saccharum officinarum var. BL 4. During the initial culture, expiant browning was controlled by presoaking 30- to 50-mm stem sets, having one bud each, for 4 to 5 hr in 5% Clorox. During subculture, the death of in vitro-produced plantlets because of browning was controlled by keeping the cultures in the dark for the first week. Maximum bud proliferation was obtained on the media having 1 to 5 μM benzylaminopurine or 20 to 40 μM kinetin. Up to 40 plantlets were obtained in 50 days. Only one culture of explanted stem pith produced callus on 1.5 Ü 10−5 M 2,4-dichlorophenoxyacetic acid medium.
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© 1985 Springer Science+Business Media New York
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Sarwar, M. (1985). In Vitro Callus Formation and Propagation of Sugarcane. In: Henke, R.R., Hughes, K.W., Constantin, M.J., Hollaender, A., Wilson, C.M. (eds) Tissue Culture in Forestry and Agriculture. Basic Life Sciences, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0378-5_53
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DOI: https://doi.org/10.1007/978-1-4899-0378-5_53
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