Histology of Structures from a Soybean (Glycine Max) Somatic Embryogenesis Protocol

  • Paulette Pierson
  • Susan Koehler
  • Martha Wright
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Globular and heart-shaped soybean (Glycine max) embryoids were obtained from the Phillips and Collins somatic embryogenesis procedure (Plant Cell Tissue Organ Culture 1:123–129). These structures, in addition to comparable stage zygotic embryos, were fixed, sectioned, and then stained with safranin-fast green. Although the somatic structures appear similar to zygotic embryos in gross morphology, the somatic structures are generally 2 to 5 times larger than comparable stage zygotic embryos. When thin sections of the somatic structures and the zygotic embryos were compared, the somatic structures lacked both a true epidermis and an internal organization capable of producing a shoot meristem.

Keywords

Cell Culture Tissue Culture Agricultural Product Somatic Embryogenesis Organ Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Paulette Pierson
    • 1
  • Susan Koehler
    • 1
  • Martha Wright
    • 1
  1. 1.Monsanto Agricultural Products CompanySt. LouisUSA

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