Abstract
Globular and heart-shaped soybean (Glycine max) embryoids were obtained from the Phillips and Collins somatic embryogenesis procedure (Plant Cell Tissue Organ Culture 1:123–129). These structures, in addition to comparable stage zygotic embryos, were fixed, sectioned, and then stained with safranin-fast green. Although the somatic structures appear similar to zygotic embryos in gross morphology, the somatic structures are generally 2 to 5 times larger than comparable stage zygotic embryos. When thin sections of the somatic structures and the zygotic embryos were compared, the somatic structures lacked both a true epidermis and an internal organization capable of producing a shoot meristem.
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© 1985 Springer Science+Business Media New York
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Pierson, P., Koehler, S., Wright, M. (1985). Histology of Structures from a Soybean (Glycine Max) Somatic Embryogenesis Protocol. In: Henke, R.R., Hughes, K.W., Constantin, M.J., Hollaender, A., Wilson, C.M. (eds) Tissue Culture in Forestry and Agriculture. Basic Life Sciences, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0378-5_49
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DOI: https://doi.org/10.1007/978-1-4899-0378-5_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0380-8
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