Regeneration of Broccoli from Buds and Leaves
Broccoli leaves and buds were used as explants on Schenk-Hildebrandt media with 8.0 mg/1 indoleacetic acid (IAA) and 0.026 mg/1 kinetin. After 30 days on the media, plantlets forming from the expiants were removed and planted in Jiffy pots. Fifteen days later they were potted in a soil mixture. The regenerated plants were greenhouse-grown to maturity in 130 days compared with a 100-day maturation time of seeded broccoli. Plantlet formation from ex-plants was 90% from buds and 40% from leaves; plantlet survival rate was 80% from buds and 70% from leaves. Histological sectioning shows vascular interconnections of the expiant to newly formed roots and shoots. Fifteen percent of all regenerated plants grown to maturity showed growth atypical for this cultivar. Although only a small number of plants has been evaluated thus far, the preliminary observation of variability in regenerated plants indicates that commercial application of tissue culture as a means of plant regeneration for broccoli may be limited.