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Rapid in vitro Propagation of Virus-Indexed Freesia

  • M. J. Foxe
  • A. E. Logan
  • F. W. Zettler
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

An efficient tissue culture method has been developed for the production of more than 50,000 virus-free gladiolus transplants within 30 weeks [A.E. Logan and F.W. Zettler (1984) Acta Horticulturae (in press)]. This method has been used, with modifications, to successfully establish a rapid in vitro propagation system for virus-indexed freesia (Freesia refracta). Using Freesia refracta cvs. Ballerina and Rose Marie, apical shoot-tips (0.5 to 0.7 mm) were established (Stage I) on an agar medium containing Murashige and Skoog salts, 2.0 mg/1 kinetin, and 0.1 mg/1 naphthaleneacetic acid (NAA). Plantlets were transferred to a similar medium containing 4.0 mg/1 kinetin and no auxin 6 to 7 weeks later for shoot proliferation (Stage II). Plantlets were subcultured on Stage II medium every 4 to 5 weeks as needed and yielded approximately 12 axillary shoots per culture. Roots developed readily in this medium suggesting that it may not be necessary to transfer the shoots to a specific root-inducing medium as is the case with gladioli. Virus indexing was accomplished using several methods, including electron microscopy and enzyme-linked immunosorbent assay.

Keywords

Cell Culture Tissue Culture Plant Pathology Agar Medium Propagation System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • M. J. Foxe
    • 1
  • A. E. Logan
    • 1
  • F. W. Zettler
    • 1
  1. 1.Department of Plant PathologyUniversity of FloridaGainesvilleUSA

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