Plant Regeneration from Embryos of Cucumis Metuliferus Cross-Pollinated with Cucumis Anguria

  • George Fassuliotis
  • B. V. Nelson
Part of the Basic Life Sciences book series (BLSC, volume 32)


The African-horned cucumber (Cucumis metuliferus Naud.) and the West India gherkin (C. anguria L.), both carrying resistance to root-knot nematodes (Meloidogyne spp.), were cross-pollinated successfully with C. metuliferus as the female parent. Embryo development was abnormal and arrested in the “rabbit-ear” stage. Occasionally, mature embryos were recovered, but they did not germinate on nutrient media tested. Presumptive hybrid plants were cultured on a basal medium containing Murashige and Skoog (MS) salts, vitamins, m-inositol (100 mg/1), casein hydrolysate (1 g/1), and sucrose (30 g/1), supplemented with indole-3-acetic acid (IAA) (5 and 10 μM) and N6-benzyladenine (BA) (5 and 10 μM), and solidified with purified agar (7 g/1). Cultures were incubated at 25°C in the dark for 7 to 14 days, transferred to 16 hr of low light (50 fc) for 5 days, and then transferred to high light (750 fc). Embryoids and leaflets differentiated on the hybrid embryo cotyledons on media containing IAA/BA (5/10, and 10/5 μM). Leaflets from 10/5 μM IAA/BA were converted to whole plants by 2 successive subcultures on 1/3 MS and 1% sucrose. The hybrid plants were abnormal and contained flattened stems with short internodes surrounded by multiple small leaves. They were chlorophyll-deficient and did not grow to maturity after transplanting to a soil mix. Further work is in progress to increase the efficiency of converting hybrid embryos to plants via plant regeneration of embryos in culture.


Plant Regeneration Nutrient Medium High Light Female Parent Hybrid Plant 
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Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • George Fassuliotis
    • 1
  • B. V. Nelson
    • 1
  1. 1.U.S. Vegetable LaboratoryCharlestonUSA

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