Somatic Embryogenesis from Commercially Important Maize Inbreds
Preliminary studies conducted in the summer of 1983 suggested that somatic embryogenesis could be achieved from certain field-grown, commercially important inbred lines of maize, as well as from crosses of these lines with A188. Three of these genotypes (B73, Mo17, and LH38) were subsequently selected for further evaluation in 1984, using greenhouse-grown donor plants and up to 8 different media. Immature embryos (ie’s) were excised at 10 to 17 days post pollination and handled according to procedures developed by C.E. Green [C.E. Green and C.A. Rhodes (1982) Plant regeneration in tissue culture of maize. In Maize for Biological Research, W. Sheridan, ed. Plant Molecular Biology Assn., Charlottesville, Virginia].