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Optimization of Electrofusion for the Formation and Culture of Protoplast Heterokaryons

  • George W. Bates
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Electrofusion has been demonstrated with a wide variety of plant protoplasts. Nonetheless, it has not been shown that the fusion products are viable, nor has the technique been refined to make it a practical alternative to fusion by PEG. Protoplasts derived from suspension cultures of Nicotiana plumbaginifolia have been fused with N. tabacum mesophyll protoplasts. By fusing mesophyll protoplasts with suspension culture protoplasts, hybrid cells can be visually identified in both living and fixed material. This system has been used to determine the optimal conditions for electrofusion and for development of a protocol for the isolation and culture of protoplast heterokaryons.

Keywords

Suspension Culture Hybrid Cell Fusion Rate Fusion Product Mesophyll Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • George W. Bates
    • 1
  1. 1.Department of Biological SciencesFlorida State UniversityTallahasseeUSA

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