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Loblolly Pine Tissue Culture: Laboratory, Greenhouse, and Field Studies

  • H. V. Amerson
  • L. J. FramptonJr.
  • S. E. McKeand
  • R. L. Mott
  • R. J. Weir
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Conifer tissue culture had its beginnings in the late 1930s (10) and shoot regeneration cultures were first noted in 1950 (4). Since that time many species (7, 17), especially those using embryonic materials for starting expiants, have been cultured. Among some of the most studied species, Pinus radiata (2), Pseudotsuga menziesii (6), Pinus pinaster (8), Picea abies (22), and Pinus taeda (18), much laboratory data are accumulating. To date, little field data have been reported on the performance of tissue-cultured conifers (14), but several conifers are now established in field plantings (21) and data should be forthcoming.

Keywords

Adventitious Shoot Genetic Gain Seed Orchard Root Initiation Hypocotyl Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Abo El-Nil, M.M. (1982) Method for asexual reproduction of coniferous trees. U.S. Patent No. 4,353,184.Google Scholar
  2. 2.
    Aitken, J., K.J. Horgan, and T.A. Thorpe (1981) Influence of expiant selection on the shoot-forming capacity of juvenile tissue of Pinus radiata. Can. J. For. Res. 11:112–117.CrossRefGoogle Scholar
  3. 3.
    Amerson, H.V., S.E. McKeand, and R.L. Mott (1981) Tissue culture and greenhouse practices for the production of loblolly pine plantlets. In Proc. 16th South. For. Tree Impr. Conf., pp. 168-173.Google Scholar
  4. 4.
    Ball, E.A. (1950) Differentiation in a callus culture of Sequoia sempervirens. Growth 14:295–325.PubMedGoogle Scholar
  5. 5.
    Brown, C.L., and R.H. Lawrence (1968) Culture of pine callus on a defined medium. For. Sci. 14:62–64.Google Scholar
  6. 6.
    Cheng, T.-Y. (1977) Factors affecting adventitious bud formation of cotyledon culture of Douglas fir. Plant Sci. Lett. 9:179–187.CrossRefGoogle Scholar
  7. 7.
    David, A. (1982) In vitro propagation of gymnosperms. In Tissue Culture in Forestry, J.M. Bonga and D.J. Durzan, eds. Martinus Nijhoff/Dr. W. Junk Publishers, The Hague, pp 72–104.CrossRefGoogle Scholar
  8. 8.
    David, A., and H. David (1977) Manifestations de diverses potentialites organogenes d’organes ou de fragments d’organes de Pin maritime (Pinus pinaster Sol.) en culture in vitro. C.R. Acad. Sci. Paris 284:627–630.Google Scholar
  9. 9.
    Durzan, D.J., and R.A. Campbell (1974) Prospects for the mass production of improved stock of forest trees by cell and tissue culture. Can. J. For. Res. 4:151–174.CrossRefGoogle Scholar
  10. 10.
    Gautheret, R.J. (1937) Nouvelles recherches sur la culture de tissu cambial. C.R. Acad. Sci. Paris 205:572–573.Google Scholar
  11. 11.
    Holley, D.L., and M.A. Veal (1977) Economie impact of fusiform rust. In Management of Fusiform Rust in Southern Pines, R.J. Dinus and R.A. Schmidt, eds. University of Florida, Gainesville, pp. 39–50.Google Scholar
  12. 12.
    McKeand, S.E. (1981) Loblolly pine tissue culture: Present and future uses in southern forestry. School of For. Res. Tech. Rep. No. 64, N.C. State University, 50 pp.Google Scholar
  13. 13.
    McKeand, S.E., and H.L. Allen (1984) Nutritional and root development factors affecting growth of tissue culture plantlets of loblolly pine. Physiol. Plant 61:523–528.CrossRefGoogle Scholar
  14. 14.
    McKeand, S.E., and L.J. Frampton, Jr. (1984) Performance of tissue culture plantlets of loblolly pine in vivo. In Proc. Intl. Symp. of Recent Adv. in For. Biotechnology, Traverse City, Missouri (in press).Google Scholar
  15. 15.
    McKeand, S.E., and L.A. Wisniewski (1982) Root morphology of loblolly pine tissue culture plantlets. In Proc. 7th No. Am. Biol. Workshop, B.A. Thielges, ed., pp. 214-219.Google Scholar
  16. 16.
    Mehra-Palta, A., R.H. Smeltzer, and R.L. Mott (1977) Hormonal control of induced organogenesis: Experiments with excised plant parts of loblolly pine. Tappi 61(1):37–40.Google Scholar
  17. 17.
    Mott, R.L. (1981) Trees. In Cloning Agricultural Plants Via In Vitro Techniques, B.V. Conger, ed. CRC Press, Boca Raton, Florida, pp. 217–254.Google Scholar
  18. 18.
    Mott, R.L., and H.V. Amerson (1981) A tissue culture process for the clonal production of loblolly pine plantlets. North Carolina Ag. Res. Ser. Tech. Bull. #271, 14. pp.Google Scholar
  19. 19.
    Patel, K.R., and T.A. Thorpe (1984) In vitro differentiation of plantlets from embryonic expiants of lodgepole pine (Pinus contorta Dougl. ex. Loud.). Plant Cell Tissue Organ Culture 3:131–142.CrossRefGoogle Scholar
  20. 20.
    Sommer, H.E., and C.L. Brown (1979) Applications of tissue culture to forest tree improvement. In Plant Cell and Tissue Culture: Principles and Applications, W.R. Sharp, P.O. Larsen, and V. Raghaven, eds. Ohio State university Press, Columbus, pp. 461–491.Google Scholar
  21. 21.
    Sommer, H.E., and H.Y. Wetzstein (1982) Application of tissue culture to forest tree improvement. Proc. 8th Long Ashton Symposium (in press).Google Scholar
  22. 22.
    von Arnold, S. (1982) Factors influencing formation, development and rooting of adventitious shoots from embryos of Picea abies (L.) Karst. Plant Sci. Lett. 27:275–287.CrossRefGoogle Scholar
  23. 23.
    Wisniewski, L.A., S.E. McKeand, and R.E. Brooks (1983) Growth of tissue culture plantlets of loblolly pine in a nursery and greenhouse. Proc. 17th So. For. Tree Improv. Conf., University of Georgia, Athens, pp. 186–193.Google Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • H. V. Amerson
    • 1
  • L. J. FramptonJr.
    • 1
  • S. E. McKeand
    • 1
  • R. L. Mott
    • 1
  • R. J. Weir
    • 1
  1. 1.Departments of Forestry and BotanyNorth Carolina State UniversityRaleighUSA

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