Somaclonal Variation in Progeny of Plants from Corn Tissue Cultures
Spontaneous variation in plants recovered from tissue culture has been documented for many species (1). This variation, recently termed “somaclonal variation” (1), has been seen in many types of culture systems, including ones involving protoplasts, long-term callus cultures, and fresh explants. The occurrence of somaclonal variation has both positive and negative aspects. For those concerned with in vitro propagation, it is undesirable; progeny not true-to-type are usually of little value. Spontaneous changes may also be a problem in attempts to transform plant cells; a high frequency of change not related to the experimental manipulations can complicate interpretation of results and can yield material with alterations other than the desired specific gene transfer. Although variability among cultured plant cells may reduce or obviate the need for mutagenesis prior to in vitro selection, it can again result in variants other than those being specifically selected.
KeywordsSOMACLONAL Variation Fertility Restoration Restorer Gene Hybrid Corn Oxyacetic Acid
Unable to display preview. Download preview PDF.
- 4.Brettell, R.I.S., R. Thomas, and D.S. Ingram (1980) Reversion of Texas male-sterile cytoplasm maize in culture to give fertile T-toxin resistant plants. Theor. Appl. Genet. 58:55–58.Google Scholar
- 5.Edallo, S., C. Zucchinali, M. Perenzin, and F. Salamini (1981) Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26:39–56.Google Scholar
- 6.Rice, T.B. (1982) Tissue culture induced genetic variation in regenerated maize inbreds. Proc. Annual Corn and Sorghum Research Conf. 37:148–162.Google Scholar
- 7.McCoy, T.J., and R.L. Phillips (1982) Chromosomal instability in maize (Zea mays L.) tissue cultures and sectoring in some regenerated plants. Can. J. Genet. Cytol. 24:559–565.Google Scholar
- 14.Laughnan, J.R., and S.J. Gabay (1978) Nuclear and cytoplasmic mutations to fertility in S male-sterile maize. In Maize Breeding and Genetics, D.B. Waiden, ed. John Wiley, New York, pp. 427–447.Google Scholar
- 16.Schardl, C.L., D.M. Lonsdale, D.R. Pring, and K.R. Rose (1984) Linearization of maize mitochondrial chromosomes by recombination with linear episomes. Nature 30:291–296.Google Scholar
- 17.Chourey, P.S., and R.J. Kemble (1982) Transposition event in tissue cultured cells of maize. In Plant Tissue Culture 1982, A. Fujiwara, ed. Japanese Association for Plant Tissue Culture, Tokyo, pp. 425–426.Google Scholar
- 19.Gracen, V.E., M.L. Forster, K.D. Sayre, and C.O. Grogan (1971) Rapid method for selecting resistant plants for control of southern corn leaf blight. Plant Dis. Rep. 55:469–470.Google Scholar
- 20.Gracen, V.E. (1982) Types and availability of male-sterile cytoplasms. In Maize for Biological Research, W.F. Sheridan, ed. University Press, Grand Forks, North Dakota, pp. 221–224.Google Scholar