Structural Elements of the 50 S Subunit of E. coli Ribosomes

  • Roland P. May
  • Heinrich B. Stuhrmann
  • Knud H. Nierhaus
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 27)

Abstract

The ribosome, the smallest cell organelle in all living cells, translates the genetic information which is coded as a sequence of four different nucleotides in nucleic acids (DNA and mRNA) into the sequence of the 20 different amino acids of proteins. Each triplet of nucleotides (codon) corresponds either to an amino acid or to a processing signal for the ribosome. Most of the investigations concerning protein biosynthesis are performed on the ribosome of the bacterium Escherichia coli. The main structural features of the E. coli ribosome are summarized in Table 1.

Keywords

Ribosomal Subunit Label Protein Scatter Length Density Label Component Scattering Length Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Engelman, D.M. and Moore, P.B., Proc. Natl. Acad. Sci. USA 69:1997–9 (1972).PubMedCrossRefGoogle Scholar
  2. 2.
    Engelman, D.M., Moore, P.B., and Schoenborn, B.P., Brookhaven Symp. Biol. 27:IV–20–37 (1975).Google Scholar
  3. 3.
    Glatter, O., J. Appl. Crystatallogr. 10:415–21 (1977).CrossRefGoogle Scholar
  4. 4.
    Guinier, A. and Fournet, G., “Small-Angle Scattering of X-Rays,” Wiley, New York (1955).Google Scholar
  5. 5.
    Hoppe, W., Isr. J. Chem. 10:321–33 (1972).CrossRefGoogle Scholar
  6. 6.
    Hoppe, W., J. Mol. Biol. 78:581–5 (1973).PubMedCrossRefGoogle Scholar
  7. 7.
    Hoppe, W., May, R., Stöckel, P., Lorenz, S., Erdmann, V.A., Wittmann, H.G., Crespi, H.L., Katz, J.J., and Ibel, K., Brookhaven Symp. Biol. 27:IV–38–48 (1975).Google Scholar
  8. 8.
    Ibel, K., J. Appl. Crystallogr. 9:296–309 (1976).CrossRefGoogle Scholar
  9. 9.
    Jacrot, B., and Zaccai, G., Biopolymers 20:2413–26 (1981).CrossRefGoogle Scholar
  10. 10.
    Moore, P.B., J. Appl. Crystallogr. 11:479–82 (1978).CrossRefGoogle Scholar
  11. 11.
    Moore, P.B., J. Appl. Crystallogr. 14:237–40 (1981).CrossRefGoogle Scholar
  12. 12.
    Nierhaus, K.H., Curr. Top. Microbiol. Immunol. 97:81–155 (1982).PubMedCrossRefGoogle Scholar
  13. 13.
    Nierhaus, K.H. and Dohme, F., Proc. Natl. Acad. Sci. USA 71:4713–17 (1974).PubMedCrossRefGoogle Scholar
  14. 14.
    Nierhaus, K.H., Lietzke, R., May, R.P., Nowotny, V., Schulze, H., Simpson, K., Wurmbach, P., and Stuhrmann, H.B., Proc. Natl. Acad. Sci. USA, submitted (1983).Google Scholar
  15. 15.
    Stöckel, P., May, R., Strell, I., Cejka, Z., Hoppe, W., Heumann, H., Zillig, W., Crespi, H.L., Katz, J.J., and Ibel, K., J. Appl. Crystallogr. 12:176–85 (1979).CrossRefGoogle Scholar
  16. 16.
    Stöckel, P., May, R., Strell, I., Cejka, Z., Hoppe, W., Heumann, H., Zillig, W., and Crespi, H.L., Eur. J. Biochem. 112:419–23 (1980).PubMedCrossRefGoogle Scholar
  17. 17.
    Stuhrmann, H.B., Acta Crystallogr. A26:297–306 (1970).Google Scholar
  18. 18.
    Stuhrmann, H.B. and Fuess, H., Acta Crystallogr. A32:67–74 (1976).Google Scholar
  19. 19.
    Stuhrmann, H.B. and Kirste, R.G., Z. Phys. Chem. N.F., 56:334–7 (1967).CrossRefGoogle Scholar
  20. 20.
    Stuhrmann, H.B., Haas, J., Ibel, K., de Wolf, B., Koch, M.H.J., Parfait, R., and Crichton, R.R., Pröc. Natl. Acad. Sci. USA 73:2379–83 (1976).PubMedCrossRefGoogle Scholar
  21. 21.
    Traub, P. and Nomura, M., Proc. Natl. Acad. Sci. USA 59:777–84 (1968).PubMedCrossRefGoogle Scholar
  22. 22.
    Ramakrishnan, V.R., Yabuki, S., Sillers, I-Y., Schindler, D.G., Engelman, D.M., and Moore, P.B., J. Mol. Biol. 153:739–60 (1981).PubMedCrossRefGoogle Scholar
  23. 23.
    Wittmann, H.G., Littlechild, J.A., and Wittmann-Liebold, B., in: “Ribosomes: Structure, Function, and Genetics,” G. Chambliss et al., eds., pp. 51–88, University Park Press, Baltimore (1979).Google Scholar
  24. 24.
    Moore, P.B., Engelman, D.M., Langer, J.A., Ramakrishnan, V.R., Schindler, D.G., Schoenborn, B.P., Sillers, I-Y., and Yabuki, S., See paper in this Symposium.Google Scholar
  25. 25.
    Moore, P.B., Private communication (1981).Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Roland P. May
    • 1
  • Heinrich B. Stuhrmann
    • 2
  • Knud H. Nierhaus
    • 3
  1. 1.Institut Laue-LangevinGrenoble CedexFrance
  2. 2.European Molecular Biology LaboratoryOutstation Hamburg DESYHamburgWest Germany
  3. 3.Max-Planck-Institut für Molekulare GenetikBerlin 33 (Dahlem)West Germany

Personalised recommendations