A Protein Structure is Only as Good as the Data

  • Benno P. Schoenborn
Part of the Basic Life Sciences book series (BLSC, volume 27)


In the interpretation of any protein structure derived from diffraction data, one has to try to assess the reliability of the derived atomic positions and atomic species. In the neutron case, it is possible to localize hydrogen atoms (1). This, however, just about doubles the total number of atoms to be derived with about the same number of reflections. In addition, neutron data are often of poorer statistical quality than their x-ray counterparts because of low source flux and the very high background caused by the incoherent scattering of hydrogen atoms. Thus in neutron protein crystallography there is a higher uncertainty in the measured structure factor, in addition to uncertainties in phasing and refinement.


Reciprocal Lattice Beam Divergence Detector Resolution Crystal Rotation Crystal Mosaic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Schoenborn, B.P., Cold Spring Harbor Symp. Quant. Biol. 36:569–75 (1971).CrossRefGoogle Scholar
  2. 2.
    Raghavan, N.V. and Schoenborn, B.P., See paper in this Symposium.Google Scholar
  3. 3.
    Hanson, J.C. and Schoenborn, B.P., J. Mol. Biol 153:117–46 (1981).PubMedCrossRefGoogle Scholar
  4. 4.
    Alberi, J., Fischer, J., Radeka, V., Rogers, L.C., and Schoenborn, B.P., IEE Trans. Nucl. Sci. NS-22:255–66 (1975).CrossRefGoogle Scholar
  5. 5.
    Radeka, V. and Boie, R.A., Nucl. Instrum. Methods 178:543–54 (1980).CrossRefGoogle Scholar
  6. 6.
    Cain, J.E., Norvell, J.C., and Schoenborn, B.P., Brookhaven Symp. Biol. 27:VIII–43–50 (1975).Google Scholar
  7. 7.
    Spencer, S. and Kossiakoff, A., J. Appl. Crystallogr. 13:563 (1980).CrossRefGoogle Scholar
  8. 8.
    Sjolin, L. and Wlodawer, A., Acta Crystallogr. A37:594 (1981).Google Scholar
  9. 9.
    Schoenborn, B.P. Acta Crystallogr. A39: in press (1983).Google Scholar
  10. 10.
    Cooper, M.J. and Nathans, R., Acta Crystallogr. A24:619 (1968).Google Scholar
  11. 11.
    Werner, S.A., Acta Crystallogr. A27:665 (1971).Google Scholar
  12. lia. Nielson, M. and Bjerrum, H., Acta Crystallogr. A25:547 (1969).Google Scholar
  13. 12.
    Phillips, S.E.V. and Schoenborn, B.P., Nature 242;81 (1981).CrossRefGoogle Scholar
  14. 13.
    Schoenborn, B.P. and Raghavan, N.V., in: “The Neutron and Its Applications 1982,” P. Schofield, ed., Inst. Phys. Conf. Ser. 64, pp. 361-4, London (1983).Google Scholar
  15. 14.
    Koeppe, R. and Schoenborn, B.P., Manuscript in preparation (1983).Google Scholar
  16. 15.
    Kossiakoff, A.A. and Spencer, S.A., Biochemistry 20:6462 (1981).PubMedCrossRefGoogle Scholar
  17. 16.
    Teeter, M. and Kossiakoff, A.A., See paper in this Symposium.Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Benno P. Schoenborn
    • 1
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA

Personalised recommendations