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Hepatitis B pp 33-43 | Cite as

Hepatitis B Viruses

  • Jesse Summers

Abstract

Hepatitis B virus is a small DNA-containing virus that causes persistent noncytopathic infections of the liver. Infected hepatocytes continually secrete viral specific particles that accumulate to high levels (1013/ml) in the blood. These particles (Figure 1) are of two types: i) noninfectious particles consisting of excess viral coat protein (HBsAg) and containing no nucleic acid, and ii) lower amounts (1010/ml) of infectious, DNA-containing particles (Dane particles) consisting of a 27 nm nucleocapsid core (HBcAg) around which is assembled an envelope containing the major viral coat protein, carbohydrate, and lipid. The DNA genome is about 3000 nucleotides in length, is circular (1) and partly single stranded, containing an incomplete plus strand (2) (Figure 1). The incomplete plus strand is complexed with a DNA polymerase in the virion which, under appropriate in vitro conditions, can elongate it using the complete minus strand as the template. These morphological and structural features distinguish hepatitis B viruses from all known classes of DNA-containing viruses.

Keywords

Viral Coat Protein Minus Strand Woodchuck Hepatitis Virus Dane Particle Strand Polarity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Robinson, W. S., Clayton, D. A., and Greenman, R. L. (1974). DNA of a human hepatitis B virus candidate. J. Virol. 14:384–391.PubMedGoogle Scholar
  2. 2.
    Summers, J., O’Connell, A., and Millman, I. (1975). Genome of hepatitis B virus: restriction enzyme cleavage and structure of the DNA isolated from Dane particles. Proc. Natl. Acad. Sci. (USA) 72:4597–4601.CrossRefGoogle Scholar
  3. 3.
    Summers, J. and Mason, W. S. (1982). Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell 29:403–415.PubMedCrossRefGoogle Scholar
  4. 4.
    Molnar-Kimber, K., Summers, J., Taylor, J. M., and Mason, W. S. (1983). Protein covalently bound to Minusstrand DNA intermediates of duck hepatitis B virus. J. Virol. 45:165–172.PubMedGoogle Scholar
  5. 5.
    Galibert, F., Chen, T. N., and Mandart, E. (1982). Nucleotide sequence of a cloned woodchuck hepatitis virus genome: comparison with the hepatitis B virus sequence. J. Virol. 41:51–65.PubMedGoogle Scholar
  6. 6.
    Summers, J. (1981). The recently described animal virus models for human hepatitis B virus. Hepatology 1:179–183.PubMedCrossRefGoogle Scholar
  7. 7.
    Beasley, R. P., Lin, C-C., Hwang, L-Y, and Chien, C-S. (1981). Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22,707 men in Taiwan. Lancet 2:1129–1132.Google Scholar
  8. 8.
    Brechot, C., Pourcel, C., Louise, A., Rain, B., and Tiollais, P. (1980). Presence of integrated hepatitis B virus DNA in cellular DNA of hepatocellular carcinoma. Nature 286:533–535.PubMedCrossRefGoogle Scholar
  9. 9.
    Shafritz, D. A., Shouval, D., Sherman, H. I., Hadziyannis, S. J., and Kew, M. (1981). Integration of hepatitis B viral DNA into the genome of liver cells in chronic liver disease and hepatocellular carcinoma. N. Engl. J. Med. 305:1067–1073.PubMedCrossRefGoogle Scholar
  10. 10.
    Ogston, C. W., Jonak, G. J., Rogler, C. E., Astrin, S. M., and Summers, J. (1982). Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from hepatocellular carcinomas of wood-chucks. Cell 29:385–394.PubMedCrossRefGoogle Scholar
  11. 11.
    Rogler, C. E. and Summers, J. Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei. J. Virol, (in press).Google Scholar
  12. 12.
    Brechot, C., Hadchouel, M., Scotto, J., Fonck, M., Potet, F., Vyas, G., and Tiollais, P. (1981). State of hepatitis B virus DNA in hepatocytes of patients with HBsAg positive and HBsAg negative liver disease. Proc. Natl. Acad. Sci. (USA) 78:3906–3910.CrossRefGoogle Scholar
  13. 13.
    London, W. T. (1981). Primary hepatocellular carcinoma—etiology, pathogenesis, and prevention. Human Path. 12:1085–1097.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Jesse Summers
    • 1
  1. 1.Institute for Cancer ResearchFox Chase Cancer CenterPhiladelphiaUSA

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