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Monoclonal Antibody-Based Quantification and Repair Analysis of Specific Alkylation Products in DNA

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Technologies for Detection of DNA Damage and Mutations

Abstract

Exposure of cells to DNA-reactive agents (exogenous and endogenous carcinogens and mutagens; cancer chemotherapeutic compounds) results in a variety of potentially mutagenic and/or cytotoxic modifications of genomic DNA (Singer and Grunberger, 1983; Hemminki and Ludlum, 1984; Rajewsky, 1989; Loeb, 1989). The molecular nature of specific DNA lesions (e.g., carcinogen-DNA adducts, DNA modifications caused by UV light or oxygen radicals) is dictated by the structure and chemical reactivity of the causative agent and, therefore, represents a “genomic fingerprint” (Singer and Grunberger, 1983; Basu and Essigmann, 1988; Rajewsky, 1989). The analysis of agent-specific DNA modifications is of considerable importance for the molecular epidemiology of carcinogen exposure as well as for the pre- and intratherapeutic dosimetry of exposure to anticancer agents (see, e.g., Umbenhauer et al., 1985; Huh et al., 1989; Groopman et al., 1991; Müller et al., 1994)

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Author notes

  1. Frank Seiler

    Present address: Institute of Hygiene and Occupational Medicine, University of Essen Medical School, D-45122, Essen, Germany

Authors and Affiliations

  1. Institute of Cell Biology (Cancer Research), West German Cancer Center Essen, University of Essen Medical School, D-45122, Essen, Germany

    Jürgen Thomale, Jörg Engelbergs, Frank Seiler & Manfred F. Rajewsky

Authors
  1. Jürgen Thomale
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  2. Jörg Engelbergs
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  3. Frank Seiler
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  4. Manfred F. Rajewsky
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Editor information

Editors and Affiliations

  1. Department of Biology, Beckman Research Institute of the City of Hope, Duarte, California, 91010, USA

    Gerd P. Pfeifer

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Thomale, J., Engelbergs, J., Seiler, F., Rajewsky, M.F. (1996). Monoclonal Antibody-Based Quantification and Repair Analysis of Specific Alkylation Products in DNA. In: Pfeifer, G.P. (eds) Technologies for Detection of DNA Damage and Mutations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0301-3_7

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  • DOI: https://doi.org/10.1007/978-1-4899-0301-3_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4899-0303-7

  • Online ISBN: 978-1-4899-0301-3

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