Monoclonal Antibody-Based Quantification and Repair Analysis of Specific Alkylation Products in DNA

  • Jürgen Thomale
  • Jörg Engelbergs
  • Frank Seiler
  • Manfred F. Rajewsky


Exposure of cells to DNA-reactive agents (exogenous and endogenous carcinogens and mutagens; cancer chemotherapeutic compounds) results in a variety of potentially mutagenic and/or cytotoxic modifications of genomic DNA (Singer and Grunberger, 1983; Hemminki and Ludlum, 1984; Rajewsky, 1989; Loeb, 1989). The molecular nature of specific DNA lesions (e.g., carcinogen-DNA adducts, DNA modifications caused by UV light or oxygen radicals) is dictated by the structure and chemical reactivity of the causative agent and, therefore, represents a “genomic fingerprint” (Singer and Grunberger, 1983; Basu and Essigmann, 1988; Rajewsky, 1989). The analysis of agent-specific DNA modifications is of considerable importance for the molecular epidemiology of carcinogen exposure as well as for the pre- and intratherapeutic dosimetry of exposure to anticancer agents (see, e.g., Umbenhauer et al., 1985; Huh et al., 1989; Groopman et al., 1991; Müller et al., 1994)


Alkylation Product Specific Gene Sequence Target Gene Sequence IARC Scientific Publ Specific Gene Fragment 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Jürgen Thomale
    • 1
  • Jörg Engelbergs
    • 1
  • Frank Seiler
    • 1
  • Manfred F. Rajewsky
    • 1
  1. 1.Institute of Cell Biology (Cancer Research), West German Cancer Center EssenUniversity of Essen Medical SchoolEssenGermany

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