Ligation-Mediated PCR for Analysis of Oxidative DNA Damage
Reactive oxygen species (ROS), including Superoxide anion, hydrogen peroxide (H2O2), hydroxyl radical, and singlet oxygen, may play an important role in promoting aging and neoplastic transformation (reviewed in Breimer, 1990; Floyd, 1990; Halliwell and Gutteridge, 1990; Piette, 1991; Ames et al., 1993; Guyton and Kensler, 1993; Nohl, 1993). Part of this role may be mediated by ROS-induced DNA mutations at critical sites. ROS, which are produced by any oxidative stress, are known to cause promutagenic damage due to a direct interaction of hydroxyl radicals and singlet oxygen with DNA (Breimer, 1990). ROS can be produced by a variety of exogenous and intracellular mechanisms, including ionizing radiation, cigarette smoke, air pollutants, toxins, UV light, inflammation, and intracellular metabolism (Guyton and Kensler, 1993). Ames (1987) has estimated that each human cell sustains an average of 103 “oxidative hits” each day.
KeywordsSodium Dodecyl Sulfate Strand Break Break Frequency Abasic Site Sequence Ladder
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