Ligation-Mediated PCR for Analysis of Oxidative DNA Damage

  • Régen Drouin
  • Henry Rodriguez
  • Gerald P. Holmquist
  • Steven A. Akman


Reactive oxygen species (ROS), including Superoxide anion, hydrogen peroxide (H2O2), hydroxyl radical, and singlet oxygen, may play an important role in promoting aging and neoplastic transformation (reviewed in Breimer, 1990; Floyd, 1990; Halliwell and Gutteridge, 1990; Piette, 1991; Ames et al., 1993; Guyton and Kensler, 1993; Nohl, 1993). Part of this role may be mediated by ROS-induced DNA mutations at critical sites. ROS, which are produced by any oxidative stress, are known to cause promutagenic damage due to a direct interaction of hydroxyl radicals and singlet oxygen with DNA (Breimer, 1990). ROS can be produced by a variety of exogenous and intracellular mechanisms, including ionizing radiation, cigarette smoke, air pollutants, toxins, UV light, inflammation, and intracellular metabolism (Guyton and Kensler, 1993). Ames (1987) has estimated that each human cell sustains an average of 103 “oxidative hits” each day.


Sodium Dodecyl Sulfate Strand Break Break Frequency Abasic Site Sequence Ladder 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Régen Drouin
    • 1
  • Henry Rodriguez
    • 2
  • Gerald P. Holmquist
    • 1
  • Steven A. Akman
    • 2
  1. 1.Division of BiologyBeckman Research Institute of the City of HopeDuarteUSA
  2. 2.Department of Medical Oncology and Therapeutics ResearchCity of Hope National Medical CenterUSA

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