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Ligation-Mediated PCR for Analysis of UV Damage

  • Silvia Tornaletti
  • Gerd P. Pfeifer

Abstract

UV irradiation of DNA produces two major types of DNA lesions:the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine (6−4) pyrimidone photoproducts [(6−4) photoproducts]. Cyclobutane dimers are formed between the 5,6 bonds of two adjacent pyrimidines, 5′-TpT, 5′-TpC, 5′-CpT, or 5′-CpC. The (6−4) photoproducts have a covalent bond between positions 6 and 4 of two adjacent pyrimidines, and are detected most frequently at 5′-TpC and 5′-CpC sequences. The (6−4) photoproducts are formed at a rate of approximately 30% of that of CPDs and this ratio appears to be DNA sequence-dependent (Mitchell and Nairn, 1989). Both photoproducts are mutagenic in Escherichia coli and in mammalian cells (Brash, 1988).

Keywords

Pyrimidine Dimer Cyclobutane Pyrimidine Dimer DHFR Gene Saran Wrap Endonuclease Versus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Silvia Tornaletti
    • 1
  • Gerd P. Pfeifer
    • 1
  1. 1.Department of BiologyBeckman Research Institute of the City of HopeDuarteUSA

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