Ligation-Mediated PCR for Analysis of UV Damage
UV irradiation of DNA produces two major types of DNA lesions:the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine (6−4) pyrimidone photoproducts [(6−4) photoproducts]. Cyclobutane dimers are formed between the 5,6 bonds of two adjacent pyrimidines, 5′-TpT, 5′-TpC, 5′-CpT, or 5′-CpC. The (6−4) photoproducts have a covalent bond between positions 6 and 4 of two adjacent pyrimidines, and are detected most frequently at 5′-TpC and 5′-CpC sequences. The (6−4) photoproducts are formed at a rate of approximately 30% of that of CPDs and this ratio appears to be DNA sequence-dependent (Mitchell and Nairn, 1989). Both photoproducts are mutagenic in Escherichia coli and in mammalian cells (Brash, 1988).
KeywordsPyrimidine Dimer Cyclobutane Pyrimidine Dimer DHFR Gene Saran Wrap Endonuclease Versus
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