Progress Toward a Transcript Map of the Human Genome

  • Anthony R. Kerlavage
  • Mark D. Adams
Part of the Stadler Genetics Symposia Series book series (SGSS)


A major goal of the human genome project is the identification and mapping of the complete complement of human genes. Single-pass, partial sequencing of cDNA clones to generate Expressed Sequence Tags (ESTs) has provided a rapid method of gene discovery (Adams et al., 1991), which has been widely applied in humans and other species (Bogusky et al., 1993). In addition, the EST strategy provides a key resource for gene mapping (Polymeropoulos et al., 1993); Durkin et al., 1994; Korenberg et al., 1995). The Institute for Genomic Research (TIGR) has sequenced ESTs from over 300 human cDNA libraries made from single cells, fetal and embryonic tissues, a large number of adult organs and tissues, and cancerous tissues (Adams et al., 1995). The combination of data on gene expression and putative gene functions inferred from sequence similarity provides a powerful means of assessing the transcriptional activity of the genome in the cells and tissues of an organism.


Radiation Hybrid Laboratory Information Management System eDNA Library Disease Gene Discovery Putative Gene Function 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Anthony R. Kerlavage
    • 1
  • Mark D. Adams
    • 1
  1. 1.9712 Medical Center DriveThe Institute for Genomic ResearchRockvilleUSA

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