Apoptotic Cell Death in the Chicken Bursa of Fabricius

  • Karen A. Jacobsen
  • Eustache Paramithiotis
  • Don L. Ewert
  • Michael J. H. Ratcliffe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 406)


The bursa of Fabricius is the site of primary B cell development in birds, and has been reviewed extensively elsewhere1–3. It is productively colonized during embryonic life by a small number (<50,000) of B lineage committed precursors which undergo immunoglobulin gene rearrangements either prior to their migration to the bursa or within the bursal rudiment during a short window of time in embryonic development. By about day 18 of embryonic development (day e18) all Ig gene rearrangements in the chicken have been completed and the adult B cells compartment is derived from this small pool of rearranged B cell precursors4.


Apoptotic Cell Death Emigration Rate Infected Chicken Normal Chicken Phagocytic Clearance 
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  1. I. M.J.H. Ratcliffe, Development of the avian B lymphocyte lineage, CRC Crit. Rev. Poult. Biol. 2: 207 (1989).Google Scholar
  2. 2.
    J.-C. Weill, and C.-A. Reynaud, The chicken B cell compartment, Science (Wash. DC) 238: 1094 (1987).CrossRefGoogle Scholar
  3. 3.
    M.J.H. Ratcliffe, and E. Paramithiotis, The end can justify the means, Semin. Immunol. 2: 165 (1990).Google Scholar
  4. 4.
    W.T. McCormack, L.W. Tjoelker, and C.B. Thompson, Avian B-cell development: generation of an immunoglobulin repertoire by gene conversion. Ann. Rev. Immunol. 9: 219 (1991).CrossRefGoogle Scholar
  5. 5.
    M.J.H. Ratcliffe, and K.A. Jacobsen, Rearrangement of chicken immunoglobulin genes in chicken B cell development, Semin. Immunol. 6: 175 (1994).PubMedCrossRefGoogle Scholar
  6. 6.
    M.J.H. Ratcliffe, and L. Tkalec, Cross-linking of the surface immunoglobulin on lymphocytes from the bursa of Fabricius results in second messenger generation. Eur. J. Immunol. 20: 1073 (1990).PubMedCrossRefGoogle Scholar
  7. 7.
    J.D. Reynolds, Mitotic rate maturation in the Peyer’s patches of fetal sheep and in the bursa of Fabricius of the chick embryo, Eue. J. Immunol. 17: 503 (1987)CrossRefGoogle Scholar
  8. 8.
    E. Paramithiotis, and M.J.H. Ratcliffe, B cell emigration directly from the cortex of lymphoid follicles in the bursa of Fabricius, Eue. J. Immunol. 24: 458 (1994).CrossRefGoogle Scholar
  9. 9.
    S. Ekino, Role of environmental antigens in B cell proliferation in the bursa of Fabricius at neonatal stage. Eur. J. Immunol. 23: 772 (1993).PubMedCrossRefGoogle Scholar
  10. 10.
    P.M. Lydyard, G.E. Grossi, and M.D. Cooper, Ontogeny of B cells in the chicken I. Sequential development of clonal diversity in the bursa, J. Exp. Med. 144: 79 (1976).PubMedCrossRefGoogle Scholar
  11. 11.
    O. Lassila, Emigration of B cells from the chicken bursa of Fabricius, Eur. J. Immunol. 19: 955 (1989).PubMedCrossRefGoogle Scholar
  12. 12.
    E. Paramithiotis, and M.J.H. Ratcliffe, Bursa-dependent subpopulations of peripheral blood B lymphocytes in chicken blood, Eue. J. Immunol. 23: 96 (1993).CrossRefGoogle Scholar
  13. 13.
    B. Motyka, and J.D. Reynolds, Apoptosis is associated with the extensive B cell death in the sheep ileal Peyer’s patches and the chicken bursa of Fabricius: a possible role in B cell selection, Eur. J. Immunol. 21: 1951 (1991).PubMedCrossRefGoogle Scholar
  14. 14.
    E. Paramithiotis, K.A. Jacobsen, and M.J.H. Ratcliffe, Loss of surface immunoglobulin expression precedes B cell death by apoptosis in the bursa of Fabricius. J. Exp. Med. 181: 105 (1995).PubMedCrossRefGoogle Scholar
  15. 15.
    J.-M. Buerstedde, C.-A. Reynaud, E.H. Humphries, W. Olsen, D.L. Ewert, and J.-C. Weill, Light chain gene conversion continues at a high rate in an ALV-induced cell line, EMBO J. 9: 921 (1990).PubMedGoogle Scholar
  16. 16.
    M.J.H. Ratcliffe, Ligation of cell surface immunoglobulin in the chicken bursa of Fabricius, Res. Immunol. 144: 421 (1993).CrossRefGoogle Scholar
  17. 17.
    S. Cory, Regulation of lymphocyte survival by the bcl-2 gene family, Ann. Rev. Immunol. 13: 513 (1995).CrossRefGoogle Scholar
  18. 18.
    I. Givol, I. Tsarfaty, J. Resau, S. Rulong, P.P. da Silva, G. Nasioulas, J. DuHadaway, S.H. Hughes, and D.L. Ewert. Bcl-2 expressed using a retroviral vector is localized primarily in the nuclear membrane and the endoplasmic reticulum of chicken embryo fibroblasts, Cell Growth Diff. 5: 419 (1994).PubMedGoogle Scholar
  19. 19.
    C.J. Petropoulos, and S.H. Hughes, Replication-competent retrovirus vectors for the transfer and expression of gene cassettes in avian cells, J. Virol. 65: 3728 (1991).PubMedGoogle Scholar
  20. 20.
    C.J. Petropoulos, W. Payne, D.W. Salter, and S.H. Hughes, Using avian retroviral vectors for gene transfer, J. Virol. 66: 3391 (1992).PubMedGoogle Scholar
  21. 21.
    T. Benatar, S. Iacampo, L. Tkalec, and M.J.H. Ratcliffe, Expression of immunoglobulin genes in the avian embryo bone marrow revealed by retroviral transformation. Eur. J. Immunol. 21: 2529 (1991).PubMedCrossRefGoogle Scholar
  22. 22.
    E. Lagasse, and I.L. Weissman, bc1–2 inhibits apoptosis of neutrophils but not their engulfment by macrophages. J. Exp. Med. 179: 1047 (1994).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Karen A. Jacobsen
    • 1
  • Eustache Paramithiotis
    • 2
  • Don L. Ewert
    • 3
  • Michael J. H. Ratcliffe
    • 1
  1. 1.Department of Microbiology and ImmunologyMcGill UniversityMontrealCanada
  2. 2.Division of Developmental and Clinical ImmunologyUniversity of Alabama at BirminghamBirminghamUSA
  3. 3.The Wistar InstitutePhiladelphiaUSA

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