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Tissue Immunoblot Procedure for Detection of Neotyphodium Coenophialum in Tall Fescue

Optimization of Time Requirements
  • E. E. HiattIII
  • N. S. Hill
  • J. H. Bouton

Abstract

Published tissue immunoblot protocols for detecting Neotyphodium coenophialum are for polyclonal antibodies (Gwinn et al., 1991; An et al. 1993) and require approximately 24 h to conduct. Commercial acceptance of immunoblot technology may be more widespread if the protocol could be shortened to accommodate labor costs and/or scheduling. The objective of this experiment was to evaluate the time requirements of the tissue immunoblot procedure and determine the minimum amount of time required to give accurate and consistent results.

Keywords

Time Requirement Tall Fescue Fungal Endophyte Tissue Print Optimum Exposure Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. An, Z.-Q., M.R. Seigel, W. Hollin, H.-F. Tsai, D. Schmidt, and C.L. Schardl. 1993. Relationships among non-Acremonium sp. fungal endophytes in five grass species. Appl. Environ. Microbiol. 59: 1540–1548.PubMedGoogle Scholar
  2. Gwinn, K.D., M.H. Collins-Shepard, and B.B. Reddick. 1991. Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology. 81: 747–748.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • E. E. HiattIII
    • 1
  • N. S. Hill
    • 1
  • J. H. Bouton
    • 1
  1. 1.Crop and Soil Sciences DepartmentUniversity of GeorgiaAthensUSAUSA

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