Immunological Detection of the Neotyphodium-Like Endophyte of Annual Ryegrass

  • J. D. Carter
  • O. J.-P. Ball
  • K. D. Gwinn
  • H. A. Fribourg

Abstract

Annual ryegrass (Lolium multiflorum) is sometimes infected with a Neotyphodium-like endophytic fungus (Latch et al. 1988). The significance of this little-studied endophyte is not thought to be great as it has not been associated with any livestock disorders, or with resistance to invertebrates, with the exception that Argentine stem weevil (Listronotus bonariensis) adults were deterred from feeding on young seedlings ([Latch et al. 1988). The Neotyphodium-like endophyte has also not been fully described taxonomically. The experiments described in this paper aimed to determine whether the Neotyphodium-like endophyte could be detected in annual ryegrass herbage (vegetative and reproductive) using a polyclonal antiserum prepared against the tall fescue (Festuca arundinacea) endophyte, N. coenophialum. This would also provide information about the serological relationship between N. coenophialum and the Neotyphodium-like endophyte.

Keywords

Endophytic Fungus Tall Fescue Polyclonal Antiserum Immunological Detection Annual Ryegrass 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Gwinn, K. D., M. H. Collins-Shephard, and B. B. Reddick. 1991. Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology. 81: 747–748.CrossRefGoogle Scholar
  2. Latch, G. C. M., M. J. Christensen, and R. E. Hickson. 1988. Endophytes of annual and hybrid ryegrasses. N.Z. J. Agric. Res. 31: 57–63.CrossRefGoogle Scholar
  3. Reddick, B. B., and M. H. Collins. 1988. An improved method for detection of Acremonium coenophialum in tall fescue plants. Phytopathology. 78: 418–420.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • J. D. Carter
    • 1
    • 2
  • O. J.-P. Ball
    • 1
    • 2
  • K. D. Gwinn
    • 1
    • 2
  • H. A. Fribourg
    • 1
    • 2
  1. 1.Department Entomology and Plant PathologyUniversity of TennesseeKnoxvilleUSA
  2. 2.AgResearchRuakura Agricultural Research CenterHamiltonNew Zealand

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