Immunological Detection of the Neotyphodium-Like Endophyte of Annual Ryegrass
Annual ryegrass (Lolium multiflorum) is sometimes infected with a Neotyphodium-like endophytic fungus (Latch et al. 1988). The significance of this little-studied endophyte is not thought to be great as it has not been associated with any livestock disorders, or with resistance to invertebrates, with the exception that Argentine stem weevil (Listronotus bonariensis) adults were deterred from feeding on young seedlings ([Latch et al. 1988). The Neotyphodium-like endophyte has also not been fully described taxonomically. The experiments described in this paper aimed to determine whether the Neotyphodium-like endophyte could be detected in annual ryegrass herbage (vegetative and reproductive) using a polyclonal antiserum prepared against the tall fescue (Festuca arundinacea) endophyte, N. coenophialum. This would also provide information about the serological relationship between N. coenophialum and the Neotyphodium-like endophyte.
KeywordsEndophytic Fungus Tall Fescue Polyclonal Antiserum Immunological Detection Annual Ryegrass
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