Abstract
Alzheimer’s disease (AD) is a chronic neurodegenerative disorder which is accompanied by memory loss and defects in cognitive function. The features in AD brains include extracellular senile plaques, intracellular neurofibrillary tangles, and amyloid proteins deposited in walls of cerebral and meningeal blood vessels. The major component of these pathognomonic lesions of AD has been identified as a 39–43 amino acid long peptide called beta amyloid peptide (Aβ). Aβ is derived from the amyloid β precursor protein (βAPP)1. The thesis that Aβ deposition plays a central role in the pathogenesis of AD has garnered increasing attention. It has been demonstrated that Aβ either is directly neurotoxic to neurons in culture,2,3 or potentiates neuronal vulnerability to excitatory neurotoxins.4,5 Neurotoxic activity has been reported to be located in the fragment of Aβ, Aβ25–35.2 We hypothesize that Aβ and its fragments, such as Aβ25–35, can form ion channels in neuronal membrane, which may allow calcium entry either directly or indirectly, thus leading to neuronal death and neuropathology of AD. In this study, we will characterize the ionic channels formed by Aβ25–35. Preliminary reports of this work have appeared elsewhere.6,7,8
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
L. Berg, and J.C. Morris, Aging and dementia, in: “Neurobiology of disease,” A.L. Pearlman, and R.C. Collins, ed., Oxford University Press, New York, Oxford (1990).
B.A. Yankner, L.K. Duffy, and D.A. Kirschner, Neurotrophic and neurotoxic effects of amyloid β protein: reversal by tachykinin neuropeptides. Science, 25:279–282 (1990).
N.W. Kowall, M.F. Beal, J. Busciglio, L.K. Duffy, and B.A. Yankner, An in vivo model for the neurodegenerative effects of β amyloid and protection by substance P. Proc. Natl. Acad. Sci. USA, 88:7247–7251 (1991).
J. Koh, L.L. Yang, and C.W. Cotman, β amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage. Brain Res., 533:315–320 (1990).
M.P. Mattson, B. Cheng, D. Davis, K. Bryant, I. Lieberburg, and R.E. Rydel, β-amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitoxicity. J. Neurosci., 12:376–389 (1992).
P. Marshall, T. Mirzabekov, W.L. Yuan, M. Carman, I. Lieberburg, and B.L. Kagan, Channels in lipid bilayers induced by a fragment of the β-amyloid peptide. Biophys. J., 64(pt2):A94 (1993).
T. Mirzabekov, M. Lin and B.L. Kagan, Ion permeability properties of lipid bilayers containing amyloid beta-peptide and its fragments. Biophys. J., 66(pt2):A430 (1994).
T. Mirzabekov, M. Lin, W. Yuan, P.J. Marshall, M. Carman, K. Tomaselli, I. Lieberburg, and B.L. Kagan, Channel formation in planar lipid bilayers by a neurotoxic fragment of the beta-amyloid peptide. Biochem. Biophys. Res. Commun., 202:1142–1148 (1994).
B.L. Kagan, and Y. Sokolov, The use of lipid bilayer membranes to detect pore formation by toxins. Methods in Enzymol., 235:699–713 (1994).
O. Alvarez, How to set up a bilayer system, in: “Ion channels reconstitution,” C. Miller, ed., Plenum Press, New York (1986).
C.J. Pike, A.J. Walencewicz, C.G. Glabe, and C.W. Cotman, In vitro aging of β-amyloid protein causes peptide aggregation and neurotoxicity. Brain Res., 563:311–314 (1991).
M.A. Simmons and C.R. Schneider Amyloid β peptides act directly on single neurons. Neurosci. Lett., 150:133–136 (1993).
B. Carette, P. Poulain, and A. Delacourte, Electrophysiological effects of 25–35 amyloid-β-protein on guinea-pig lateral septal neurons. Neurosci. Lett., 151:111–114 (1993).
P. Seubert, C. Vigo-Pelfrey, F. Esch, M. Lee, H. Dovey, D. Davis, S. Sinha, M. Schlossmacher, J. Whaley, C. Swindlehurst, R. McCormack, R. Wolfen, D. Selkoe, I. Lieberburg, and D. Schenk, Isolation and quantification of soluble Alzheimer’s beta-peptide from biological fluids. Nature, 359:325–327 (1992).
N. Arispe, E. Rojas, and H.B. Pollard, Alzheimer disease amyloid β protein forms calcium channels in bilayer membranes: blockade by tromethamine and aluminum. Proc. Natl. Acad Sci. USA, 90:567–571 (1993).
N. Arispe, H.B. Pollard, and E. Rojas, Giant multilevel cation channels formed by Alzheimer disease amyloid β-protein[AβP-(1–40)] in bilayer membranes. Proc. Natl. Acad Sci. USA, 90:10573–10577 (1993).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Lin, Mc., Mirzabekov, T., Kagan, B. (1996). Channel Formation by a Neurotoxic Beta Amyloid Peptide, Aβ25–35. In: Fiskum, G. (eds) Neurodegenerative Diseases. GWUMC Department of Biochemistry and Molecular Biology Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0209-2_39
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0209-2_39
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0211-5
Online ISBN: 978-1-4899-0209-2
eBook Packages: Springer Book Archive