Apolipoprotein E Uptake is Increased by Beta-Amyloid Peptides and Reduced by Blockade of the LDL Receptor
Apolipoprotein E (apoE) is a lipid-binding, 37 kDa, 299 amino acid glycoprotein involved in cholesterol and phospholipid transport and metabolism. ApoE mediates the uptake of lipid complexes through interaction with the apoB/ApoE (LDL) receptor and other receptors1. The LDL receptor pathway consists of cell surface binding of apoE-containing lipoproteins followed by internalization and degradation of the lipoprotein by a lysosomal pathway2. In the peripheral nervous system, apoE has been proposed to be involved in the transport of cholesterol in repair, growth and maintenance of membranes during development or following injury3,4. In the central nervous system, ApoE released from astrocytes in response to injury such as entorhinal cortex lesions in the rat plays a pivotal role in the redistribution of cholesterol and phospholipids during synaptic remodeling and compensatory synaptogenesis5,6.
KeywordsPrimary Neuronal Culture Cell Surface Binding Cholinergic Dysfunction Human apoE3 Allele Copy Number
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