Taurine 2 pp 69-71 | Cite as

Taurine Production in Rat Primary Hepatocytes

  • Jun Ohta
  • Martha H. Stipanuk
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)


Cysteine metabolism in rat hepatocytes is highly regulated in response to the dietary levels of protein and sulfur amino acids1, 2. Primary cultures of rat hepatocytes might be useful for studies of the regulation of cysteine metabolism, including taurine production. However, conventional monolayer cultures are known to lose many of the differentiated functions of liver cells. Recently, it has been reported that hepatocytes cultured as spheroids (multicellular aggregates with well-defined cellular morphology) maintain certain highly differentiated functions of liver parenchymal cells such as secretion of albumin7, 5.


Sulfur Amino Acid Spheroid Culture Multicellular Aggregate Methionine Supplementation Half Cystine 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Jun Ohta
    • 1
    • 2
  • Martha H. Stipanuk
    • 1
  1. 1.Division of Nutritional SciencesCornell UniversityIthacaUSA
  2. 2.Department of BiochemistryOkayama University Medical SchoolOkayamaJapan

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