Taurine 2 pp 249-255 | Cite as

Effects of Chronic Taurine Treatment on the Electrical and Contractile Properties of Skeletal Muscle Fibers of Aged Rats

  • Sabata Pierno
  • Annamaria De Luca
  • Ryan J. Huxtable
  • Diana Conte Camerino
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)


Taurine has an important role in stabilizing mammalian skeletal muscle membrane. Indeed previous studies have shown that in vitro application of taurine reduces fiber excitability by a specific and concentration-dependent increase of resting Cl conductance (GCl)4, 20, this parameter largely contributing to the electrical stability of sarcolemma21. A better evaluation of the role of taurine in skeletal muscle derives from studies on taurine-de-pleted muscles. Indeed chronic treatment with guanidinoethane sulfonate (GES), an inhibitor of taurine transport15, caused a fall in muscular taurine content and a marked decrease of GCl along with an increase of excitability of rat skeletal muscle fibers9, 19. Furthermore we found that the excitation-contraction coupling process of taurine-depleted muscles was significantly changed, the GES-treated fibers contracting at more negative potentials than normal controls9.


Extensor Digitorum Longus Skeletal Muscle Fiber Mechanical Threshold Aged Muscle Taurine Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adrian, R.H. and Bryant, S.H. 1974, On the repetitive discharge in myotonic muscle fibres, J. Physiol. 240:505–515.Google Scholar
  2. 2.
    Bryant, S.H. and Conte Camerino, D. 1991, Chloride channel regulation in the skeletal muscle of normal and myotonic goats, Pflügers Arch. 417:605–610.CrossRefGoogle Scholar
  3. 3.
    Camerino, D. and Bryant, S.H. 1976, Effects of denervation and colchicine treatment on the chloride conductance of rat skeletal muscle fibers, J. Neurobiol. 7:221–228.CrossRefGoogle Scholar
  4. 4.
    Conte Camerino, D., Franconi, F., Mambrini, M., Bennardini, F., Failli, P., Bryant, S.H. and Giotti, A. 1987, The action of taurine on chloride conductance and excitability characteristics of rat striated muscle fibers, Pharmacol. Res. Commun. 19:685–701.CrossRefGoogle Scholar
  5. 5.
    Dawson, R. and Wallace, D.R. 1992, Taurine content in tissues from aged Fischer 344 rats, Age 15:73–81.CrossRefGoogle Scholar
  6. 6.
    De Luca, A. and Conte Camerino, D. 1992, Effects of aging on the mechanical threshold of rat skeletal muscle fibers, Pflügers Arch. 420:407–409.CrossRefGoogle Scholar
  7. 7.
    De Luca, A., Mambrini, M. and Conte Camerino, D. 1990, Changes in membrane ionic conductances and excitability characteristics of rat skeletal muscle during aging, Pflügers Arch. 415:642–644.CrossRefGoogle Scholar
  8. 8.
    De Luca, A., Pierno, S., Cocchi, D. and Conte Camerino, D. 1994, Growth hormone administration to aged rats improves membrane electrical properties of skeletal muscle fibers, J. Pharmacol. Exp. Ther. 269:948–953.Google Scholar
  9. 9.
    De Luca, A., Pierno, S., Huxtable, R.J., Failli, P., Franconi, F., Giotti, A. and Conte Camerino, D. 1992, Effects of taurine depletion on membrane electrical properties of rat skeletal muscle, in: “Taurine Nutritional Value and Mechanisms of Action,” J.B. Lombardini, S.W. Schaffer and J. Azuma, eds., Plenum Press, New York, Vol 315 pp. 199–205.Google Scholar
  10. 10.
    De Luca, A., Tricarico, D., Pierno, S. and Conte Camerino, D. 1994, Aging and chloride channel regulation in rat fast-twitch muscle fibers, Pflügers Arch. 427:80–85.CrossRefGoogle Scholar
  11. 11.
    Eberhard, D.A. and Holtz, R.W. 1988, Intracellular Ca2+ activates phospholipase C, Trends Neurosci. 11:517–520.CrossRefGoogle Scholar
  12. 12.
    Eisenberg, R.S. and Gage, R.W. 1969, Ionic conductance of the surface and trasverse tubular membranes of frog sartorius fibers, J. Gen. Physiol. 53:279–297.CrossRefGoogle Scholar
  13. 13.
    Green, J.R. and Margerison, D. 1978, in: “Statistical Treatment of Experimental Data,” New York, Elsevier, pp. 86–88.Google Scholar
  14. 14.
    Huxtable, R.J. 1992, The physiological actions of taurine, Physiol. Rev. 72:101–163.Google Scholar
  15. 15.
    Huxtable, R.J., Laird, H.E. and Lippincott, S.E. 1979, The transport of taurine in the heart and the rapid depletion of tissue taurine content by guanidinoethyl sulfonate, J. Pharmacol. Exp. Ther. 211:465–471.Google Scholar
  16. 16.
    Koch, M.C., Steinmeyer, K., Lorenz, C., Ricker, K., Wolf, F., Otto, M., Zoll, B., Lehmann-Horn, F., Grzeschik, K.H. and Jentsch, T.J. 1992, The skeletal muscle chloride channel in dominant and recessive human myotonia, Science 257:797–800.CrossRefGoogle Scholar
  17. 17.
    Larsson, L. and Salviati, G. 1989, Effects of age on calcium transport activity of sarcoplasmic reticulum in fast-and slow-twitch rat muscle fibers, J. Physiol. 419:253–264.Google Scholar
  18. 18.
    Li, Y.-P. and Lombardini, J.B. 1991, Inhibition by taurine of the phosphorylation of specific synaptosomal proteins in the rat cortex: effects of taurine on the stimulation of calcium uptake in mitochondria and inhibition of phosphoinositide turnover, Brain Res. 553:89–96.CrossRefGoogle Scholar
  19. 19.
    Pierno, S., De Luca, A., Huxtable, R. J. and Conte Camerino, D. 1994, Dual effects of taurine on membrane ionic conductances of rat skeletal muscle fibers, in: “Taurine in Health and Desease,” R.J. Huxtable and D.V. Michalk, eds., Plenum Press, New York, pp. 217–224.Google Scholar
  20. 20.
    Pierno, S., Tricarico, D., De Luca, A., Campagna, F., Carotti, A., Casini, G. and Conte Camerino, D. 1994, Effects of taurine analogues on chloride channel conductance of rat skeletal muscle fibers: a structure-activity relationship investigation, Naunyn-Schmiedeberg’s Arch. Pharmacol. 349:416–421.CrossRefGoogle Scholar
  21. 21.
    Rüdel, R. and Lehmann-Horn, F. 1985, Membrane change in cells from myotonia patients, Physiol. Rev. 65:310–356.Google Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Sabata Pierno
    • 1
  • Annamaria De Luca
    • 1
  • Ryan J. Huxtable
    • 2
  • Diana Conte Camerino
    • 1
  1. 1.Unit of Pharmacology, Department of Pharmacobiology, Faculty of PharmacyUniversity of BariItaly
  2. 2.Department of Pharmacology, College of MedicineUniversity of ArizonaTucsonUSA

Personalised recommendations