Abstract
Administration of clenbuterol to rats in the drinking water over a four day period increased incorporation of [3H]leucine into muscle protein but did not result in an increase in body or muscle weight. However, both urinary and liver taurine were significantly reduced at the highest dose of clenbuterol (2 mg. kg−1 day−1). Salbutamol also reduced urinary levels of taurine in both rats and humans. The reduction in the body pool of taurine caused by β-agonists may be of concern as taurine has been shown to have protective properties.
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References
Azuma, J., Hamaguchi, T., Ohta, H., Takihara, K., Awata, N., Sawamura, A., Harada, H., Tanaka, Y., and Kishimoto, S. 1987, Calcium overload-induced myocardial damage caused by isoproterenol and by adriamycin: possible role of taurine in its prevention, in: “The Biology of Taurine: Methods and Mechanisms,” Huxtable, R.J., Franconi, F. and Giotti, A. eds., Plenum Press, New york, pp. 167–179.
Carvalho, F., Waterfield, C.J., de Lourdes Bastos, M., Ferreira, M., and Timbrell, J.A. 1995, Effect of repeated administration of salbutamol on urinary and liver taurine levels in rats, Pharmacol.Commun. 5:171–180.
DeMaster, E.G. and Redfern, B. 1987, High-performance liquid chromatography of hepatic thiols with electrochemical detection, in: “Methods in Enzymology”, Jakoby, W.B. and Griffith, O.W. eds., Academic Press, New York City, NY., pp. 110–114.
Ellman, G.L. 1959, Tissue sulfhydryl groups, Arch.Biochem.Biophys. 82:70–77.
Hasselgren, P.-O., Pedersen, P., Sax, H.C., Warner, B.W., and Fischer 1988, Current research review: Methods for studying protein synthesis and degradation in liver and skeletal muscle, J.Surg.Res. 45:389–415.
Hesketh, J.E., Campbell, G.P., Lobley, G.E., Maltin, C.A., Acamovic, F., and Palmer, R.M. 1992, Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol; Evidence for translational control, Comp.Biochem.Physiol. 102C:23–27.
Huxtable, R.J. 1992, Physiological actions of taurine, Physiol.Rev. 72:101–163.
Larsen, B.R., Grasso, D.S., and Chang, S.Y. 1980, A rapid method for taurine quantitation using high performance liquid chromatography, J.Chromatog.Sci. 18:233–236.
Lowry, O.J., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951, Protein measurement with folin phenol reagent, J.Biol.Chem. 193:265–275.
Martineau, L., Horan, M.A., Rothwell, N.J., and Little, R.A. 1992, Salbutamol, a β2-adrenoceptor agonist, increases skeletal muscle strength in young men, Clin. Sci. 83:615–621.
McElligott, M.A., Barreto, A., Jr., and Chaung, L.-Y. 1989, Effect of continuous and intermittent clenbuterol feeding on rat growth and muscle, Comp.Biochem.Physiol. 92c: 135–138.
Nakeo, T.K., Sato, T., Nakamura, T., Taguchi, K., Moriyoshi, M., and Kawaka, K. 1992, Use of a beta 2-adrenergic stimulant (clenbuterol) for eliminating night-time calving, J. Vet.Med.Sci. 54:19–22.
Potter, D.W. and Tran, T.-B. 1993, Apparent rates of glutathione turnover in rat tissues, Toxicol.Appl.Pharmacol. 120:186–192.
Reeds, P.J., Hay, S.M., Dorwood, P.M., and Palmer, R.H. 1986, Stimulation of muscle growth by clenbuterol: lack of effect on muscle protein biosynthesis, Br.J.Nutr. 56:249–258.
Rothwell, N.J. and Stock, M.J. 1987, Effect of a selective β2-adrenergic agonist (clenbuterol) on energy balance and body composition in normal and protein deficient rats, Biosci.Reps. 7:933–940.
Siedel, J., Mullering, H., and Ziegenhorn, J. 1992, Sensitive color reagent for the enzymatic determination of creatinine, Clin.Chem. 30:968–969.
Sturman, J.A. 1993, Taurine in development, Physiol.Rev. 73:119–147.
Waterfield, C.J. 1994, Determination of taurine in biological samples and isolated hepatocytes by high-performance liquid chromatography with fluorimetric detection, J.Chromatogr.B Biomed.Appl. 657:37–45.
Waterfield, C.J., Jairath, M., Asker, D.S., and Timbrell, J.A. 1995, The biochemical effects of clenbuterol: With particular reference to taurine and muscle damage, Eur.J.Pharmacol.Environ. Toxicol.Pharmacol. 293:141–149.
Waterfield, C.J., Turton, J.A., Scales, M.D., and Timbrell, J.A. 1993, Effect of various non-hepatotoxic compounds on urinary and liver taurine levels in rats, Arch.Toxicol. 67:538–546.
Waterfield, C.J., Turton, J.A., Scales, M.D.C., and Timbrell, J.A. 1992, The correlation between urinary and liver taurine levels and between predose urinary taurine and liver damage, Toxicology, 77:1–5.
Waterfield, C.J., Turton, J.A., Scales, M.D.C., and Timbrell, J.A. 1992, Reduction of liver taurine in rats by β-alanine treatment increases carbon tetrachloride toxicity, Toxicology, 77:7–20.
Yang, Y.T. and McElligott, M.A. 1989, Multiple actions of β-adrenergic agonists on skeletal muscle and adipose tissue, Biochem.J. 261:1–10.
Zelikovic, I. and Chesney, R.W. 1989, Taurine, in: “New protective roles for selected nutrients”, Spiller, G.A. and Scala, J. eds., Alan R. Liss, New York, pp. 253–297.
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Waterfield, C.J., Carvalho, F., Timbrell, J.A. (1996). Effect of Treatment with β-Agonists on Tissue and Urinary Taurine Levels in Rats. In: Huxtable, R.J., Azuma, J., Kuriyama, K., Nakagawa, M., Baba, A. (eds) Taurine 2. Advances in Experimental Medicine and Biology, vol 403. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0182-8_25
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