Taurine 2 pp 147-155 | Cite as

Protective Effect of Taurine on Indomethacin-Induced Gastric Mucosal Injury

  • Miwon Son
  • Hee Kee Kim
  • Won Bae Kim
  • Junnick Yang
  • Byong Kak Kim
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)


It has been suggested that oxygen-derived free radicals play an important role in the pathophysiology of acute gastric ulceration induced by NSAIDs and ischemia-reperfusion. Taurine is hypothesized to exert its protective effect on NSAIDs-induced gastric injury by its antioxidant properties. The protective effect of taurine on indomethacin-induced gastric mucosal lesion and its protective mechanism were investigated. Intragastric administration of 25 mg/kg of indomethacin induced hemorrhagic lesions on the glandular stomach in rats. Pretreatment with 0.25 or 0.5 g/kg of taurine one day before or for 3 days significantly reduced gastric lesion formation and inhibited the elevation of lipid peroxide level in gastric mucosa. Both resting and FMLP-induced luminol-dependent chemiluminescence of rat peritoneal neutrophils increased immediately after treatment with indomethacin. Taurine (5–20 mM) inhibited chemiluminescence of neutrophils activated by FMLP. Human neutrophils (polymorphonuclear leukocytes) adhered to the confluent monolayer of human umbilical vein endothelial cells (HUVEC) after coincubation with indomethacin. This neutrophil adhesion induced by indomethacin to HUVEC was prevented by taurine in a dose-dependent manner.

These results indicate that the protective effect of taurine against NSAIDs-induced gastric mucosal injury is due to its antioxidant effect, which inhibits lipid peroxidation and neutrophil activation.


Human Umbilical Vein Endothelial Cell Gastric Mucosal Injury Gastric Mucosal Lesion Gastric Mucosal Blood Flow Indomethacin Administration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Aruoma, O.I., Halliwell, B., Hoey, B.M., and Butler, J. 1988, The antioxidant action of taurine, hypotaurine and their metabolic precursors, Biochem. J. 256:251–255.Google Scholar
  2. 2.
    Ashey, S.W., Sonnenschein, L.A., and Cheung, L.Y. 1985, Focal gastric mucosal blood flow at the site of aspirin-induced ulceration, Am.J.Surg. 149:53–59.CrossRefGoogle Scholar
  3. 3.
    Ayene, I.S., Dodia, C., and Fisher, A.B. 1992, Role of oxygen in oxidation of lipid and protein during ischemia/reperfusion in isolated perfused rat lung, Arch.Biochem.Biophys. 296:183–189.CrossRefGoogle Scholar
  4. 4.
    Banks, M.A., Porter, D.W., Martin, W.G., and Castranova, V. 1990, Effects of in vitro ozone exposure on peroxidative damage, membrane leakage, and taurine content of rat alveolar macrophages, Toxicol.Appl.Pharmacol. 105:55–65.CrossRefGoogle Scholar
  5. 5.
    Cantin, A.M. 1994, Taurine modulation of hypochlorous acid-induced lung epithelial cell injury in vitro. Role of anion transport, J.Clin.Invest. 93:606–614.CrossRefGoogle Scholar
  6. 6.
    Dahlgren, C. 1991, Effect of different inhibitors on the intracellularly and extracellularly generated chemiluminescence induced by formylmethionyl-leucyl-phenylalanine in polymorphonuclear leukocytes. Cellular response in the presence of mannitol, benzoate, taurine, indomethacin and NDGA, J.Biolum.Chemilum. 6:29–34.CrossRefGoogle Scholar
  7. 7.
    Gimbrone, M.A., Jr., Brock, A.F., and Schafer, A. 1984, Leukotriene B4 stimulates polymorphonuclear leukocyte adhesion to cultured vascular endothelial cells, J.Clin.Invest. 74:1552–1555.CrossRefGoogle Scholar
  8. 8.
    Giri, S.N. and Wang, Q. 1992, Taurine and niacin offer a novel therapeutic modality in prevention of chemically-induced pulmonary fibrosis in hamsters, Adv.Exp.Med.Biol. 315:329–340.CrossRefGoogle Scholar
  9. 9.
    Green, T.R., Fellman, J.H., Eicher, A.L., and Pratt, K.L. 1991, Antioxidant role and subcellular location of hypotaurine and taurine in human neutrophils, Biochim.Biophys.Acta Gen.Subj. 1073:91–97.CrossRefGoogle Scholar
  10. 10.
    Hirota, M., Inoue, M., Ando, Y., and Morino, Y. 1990, Inhibition of stress-induced gastric mucosal injury by a long acting Superoxide dismutase that circulates bound to albumin, Arch.Biochem.Biophys. 280:269–273.CrossRefGoogle Scholar
  11. 11.
    Kitahora, T. and Guth, P.H. 1987, Effect of aspirin plus hydrochloric acid on the gastric mucosal microcirculation, Gastroenterology, 93:810–817.Google Scholar
  12. 12.
    McGregor, P.E., Agrawal, D.K., and Edwards, J.D. 1994, Technique for assessment of leukocyte adherence to human umbilical vein endothelial cell monolayers, J.Pharm.Mol. 32:73–77.Google Scholar
  13. 13.
    Naito, Y., Yoshikawa, T., Kaneko, T., Iinuma, S., Nishimura, S., Takahashi, S., and Kondo, M. 1993, Role of oxygen radicals in indomethacin-induced gastric mucosal microvascular injury in rats, J. Clin. Gastroenterol 17:S99–S103.CrossRefGoogle Scholar
  14. 14.
    Pasantes-Morales, H. and Cruz, C. 1984, Protective effect of taurine and zinc on peroxidation-induced damage in photoreceptor outer segments, J.Neurosci.Res. 11:303–311.CrossRefGoogle Scholar
  15. 15.
    Pasantes-Morales, H. and Cruz, C. 1985, Taurine and hypotaurine inhibit light-induced lipid peroxidation and protect rod outer segment structures, Brain Res. 330:154–157.CrossRefGoogle Scholar
  16. 16.
    Takeuchi, K., Ueshima, K., Hironaka, Y., Fujioka, Y., Matsumoto, J., and Okabe, S. 1991, Oxygen free radicals and lipid peroxidation in the pathogenesis of gastric mucosal lesions induced by indomethacin in rats, Digestion, 49:175–184.CrossRefGoogle Scholar
  17. 17.
    Vaananen, P.M., Meddings, J.B., and Wallace, J.L. 1991, Role of oxygen-derived free radicals in indomethacin-induced gastric injury, Am.J.Physiol. 261:G470–G475.Google Scholar
  18. 18.
    Wallace, J.L. and Granger, D.N. 1992, Pathogenesis of NSAID gastropathy: are neutrophils the culprits? Trends Pharmacol.Sci. 13:129–131.CrossRefGoogle Scholar
  19. 19.
    Wallace, J.L., Keenan, CM., and Granger, D.N. 1990, Gastric ulceration induced by nonsteroidal anti-inflammatory drugs is a neutrophil-dependent process, J.Physiol. 259:G462–G467.Google Scholar
  20. 20.
    Wang, Q., Hyde, D.M., and Giri, S.N. 1992, Abatement of bleomycin-induced increases in vascular permeability, inflammatory cell infiltration, and fibrotic lesions in hamster lungs by combined treatment with taurine and niacin, Lab.Invest. 67:234–242.Google Scholar
  21. 21.
    Yoshida, N., Takemura, T., Neil Granger, D., Anderson, D.C., Wolf, R.E., McIntire, L.V., and Kvietys, P.R. 1993, Molecular determinants of aspirin-induced neutrophil adherence to endothelial cells, Gastroenterology, 105:715–724.CrossRefGoogle Scholar
  22. 22.
    Yoshikawa, T., Ueda, S., Naita, Y., Takahashi, S., Oyamada, H., Morita, Y., Yoneta, T., and Kondo, M. 1989, Role of oxygen-derived free radicals in gastric mucosal injury induced by ischemia or ischemia-reperfusion in rats, Free Rad.Res.Comms. 7:285–291.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Miwon Son
    • 1
  • Hee Kee Kim
    • 1
  • Won Bae Kim
    • 1
  • Junnick Yang
    • 1
  • Byong Kak Kim
    • 2
  1. 1.Research Laboratories of Dong-A Pharmaceutical Co., Ltd.Yongin-kun, Kyungki-doKorea
  2. 2.College of PharmacySeoul National UniversitySeoulKorea

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