Advertisement

Production of Platelet-Activating Factor by Brain Microvascular Endothelial Cells

  • Kei Satoh
  • Masayuki Koyama
  • Hidemi Yoshida
  • Shigeru Takamatsu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 416)

Abstract

Vascular endothelial cells produce platelet-activating factor (PAF) in response to various agonists, and endothelial PAF is one of the key molecules regulating the interactions between blood cells and the vascular wall.1) To investigate the functional specificity of the vascular endothelium in brain, we have established pure cultures of porcine brain microvascular endothelial cells. Endothelial cells from porcine aorta were also cultured, and using these cultures we have conducted a comparative study on PAF production. Since endothelial PAF production is closely related to the adherence of white blood cells to the endothelium,1) we also study the adhesion of human polymorphonuclear neutrophils (PMNs) to the cultured cells.

Keywords

Microvascular Endothelial Cell Aortic Endothelial Cell Brain Endothelial Cell Brain Microvascular Endothelial Cell Extracellular Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Zimmerman GA, Prescott SM, McIntyre TM: Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today 13: 93–100, 1992PubMedCrossRefGoogle Scholar
  2. 2.
    Satoh K, Yoshida H, Imaizumi T, Koyama M, Takamatsu S: Production of platelet-activating actor by porcine brain microvascular endothelial cells in culture. Thromb Haemostas, in pressGoogle Scholar
  3. 3.
    Prescott SM, Zimmerman GA, McIntyre TM: Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin. Proc Natl Acad Sci USA 81: 3534–3538, 1984PubMedCrossRefGoogle Scholar
  4. 4.
    Miwa M, Miyake T, Yamanaka T, Sugatani J, Suzuki Y, Sakata S, Araki Y, Matsumoto M: Characterization of serum platelet-activating factor (PAF) acetylhydrolase. Correlation of serum PAF acetylhydrolase and respiratory symptoms in asthmatic children. J Clin Invest 82: 1983–1991, 1988PubMedCrossRefGoogle Scholar
  5. 5.
    Miwa M, Sugatani J, Ikemura T, Okamoto Y, Ino M, Saito K, Suzuki Y, Matsumoto M: Release of newly synthesized platelet-activating factor (PAF) from human polymorphonuclear leukocytes under in vivo conditions: contribution of PAF-releasing factor in serum. J Immunol 148: 872–880, 1992PubMedGoogle Scholar
  6. 6.
    Bonfanti R, Furie BC, Wagner DD: PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood 73: 1109–1112, 1989PubMedGoogle Scholar
  7. 7.
    Bowman PD, du Bois M, Dorovini-Zis K, Shivers RR: Microvascular endothelial cells from brain. In: Piper HM, ed., Cell Culture Techniques in Heart and Vessel Research. Springer-Verlag, Berlin, FRG, pp 140–157, 1990CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Kei Satoh
    • 1
  • Masayuki Koyama
    • 1
  • Hidemi Yoshida
    • 1
  • Shigeru Takamatsu
    • 1
  1. 1.Department of Pathological Physiology Institute of Neurological DiseasesHirosaki University School of MedicineHirosakiJapan

Personalised recommendations