Abstract
Vascular endothelial cells produce platelet-activating factor (PAF) in response to various agonists, and endothelial PAF is one of the key molecules regulating the interactions between blood cells and the vascular wall.1) To investigate the functional specificity of the vascular endothelium in brain, we have established pure cultures of porcine brain microvascular endothelial cells. Endothelial cells from porcine aorta were also cultured, and using these cultures we have conducted a comparative study on PAF production. Since endothelial PAF production is closely related to the adherence of white blood cells to the endothelium,1) we also study the adhesion of human polymorphonuclear neutrophils (PMNs) to the cultured cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Zimmerman GA, Prescott SM, McIntyre TM: Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today 13: 93–100, 1992
Satoh K, Yoshida H, Imaizumi T, Koyama M, Takamatsu S: Production of platelet-activating actor by porcine brain microvascular endothelial cells in culture. Thromb Haemostas, in press
Prescott SM, Zimmerman GA, McIntyre TM: Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin. Proc Natl Acad Sci USA 81: 3534–3538, 1984
Miwa M, Miyake T, Yamanaka T, Sugatani J, Suzuki Y, Sakata S, Araki Y, Matsumoto M: Characterization of serum platelet-activating factor (PAF) acetylhydrolase. Correlation of serum PAF acetylhydrolase and respiratory symptoms in asthmatic children. J Clin Invest 82: 1983–1991, 1988
Miwa M, Sugatani J, Ikemura T, Okamoto Y, Ino M, Saito K, Suzuki Y, Matsumoto M: Release of newly synthesized platelet-activating factor (PAF) from human polymorphonuclear leukocytes under in vivo conditions: contribution of PAF-releasing factor in serum. J Immunol 148: 872–880, 1992
Bonfanti R, Furie BC, Wagner DD: PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood 73: 1109–1112, 1989
Bowman PD, du Bois M, Dorovini-Zis K, Shivers RR: Microvascular endothelial cells from brain. In: Piper HM, ed., Cell Culture Techniques in Heart and Vessel Research. Springer-Verlag, Berlin, FRG, pp 140–157, 1990
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Satoh, K., Koyama, M., Yoshida, H., Takamatsu, S. (1996). Production of Platelet-Activating Factor by Brain Microvascular Endothelial Cells. In: Nigam, S., Kunkel, G., Prescott, S.M. (eds) Platelet-Activating Factor and Related Lipid Mediators 2. Advances in Experimental Medicine and Biology, vol 416. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0179-8_4
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0179-8_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0181-1
Online ISBN: 978-1-4899-0179-8
eBook Packages: Springer Book Archive