Platelet-Activating Factor is an Effector of Rapid Reactions and an Inductor of Late Responses in Immune-Mediated Injury
The reversed passive Arthus reaction (RPA) was applied to the peritoneal cavity of rats to perform the present study. In this model, antibody is locally injected, and the cognate antigen is intravenously administered. The analysis of the sequence of events that occur in the RPA reaction includes formation of immune complexes in the microvessel wall, activation of the complement cascade, migration and adherence of polymorphonuclear leukocytes (PMN) to the endothelial cells, and release of PAF from PMN which acts on endothelial cells to cause leakage1–3. Recent studies have emphasized the involvement of nitric oxide (NO) in the production of tissue injury in a model of alveolitis and dermal vasculitis analogous to RPA4–5, but the actual role of NO in immune-mediated injury has not been ascertained as yet. The purpose of this study has been to assess the effect of blocking PAF receptors on events of the RPA that occur at different times after antigen challenge.
KeywordsNitric Oxide Evans Blue Peritoneal Cell Isosorbide Dinitrate Nitrite Production
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