Influence of Hexadecylphosphocholine (Miltefosine) on Cytokine Synthesis and Biological Responses

  • T. Klenner
  • T. Beckers
  • K. Nooter
  • H. Holtmann
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 416)


Hexadecylphosphocholine, INN: Miltefosine (Figure 1), the lead compound of the alkylphosphocholines, has shown its antineoplastic activity in various in-vitro and in-vivo tumor models (Hilgard et al. 1993). After oral administration to patients miltefosine induced a remarkable leucocytosis (Pronk et al. 1994). This suggested a stimulatory effect of the compound on the hematopoietic system. To investigate this phenomenon, mouse and rat bone marrow was stimulated with either recombinant mouse/rat IL-3 or mouse GM-CSF with or without the addition of miltefosine. Additionally, Northern blot analysis was performed on human myeloid leukemic cell lines for the IL-3 and GM-CSF receptor genes and for TNFα mRNA on peripheral blood cells stimulated with concanavalin A (Con A) or lipopolysaccharid (LPS) when they were exposed to Miltefosine.


Northern Blot Analysis Cytokine Synthesis Colony Form Unit Assay eDNA Probe Human Myeloid Leukemic Cell Line 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • T. Klenner
    • 1
  • T. Beckers
    • 1
  • K. Nooter
    • 2
  • H. Holtmann
    • 3
  1. 1.ASTA Medica AGFrankfurt am MainGermany
  2. 2.Department of Medical OncologyUniversity Hospital RotterdamRotterdamThe Netherlands
  3. 3.Institute for Molecular PharmacologyMedical SchoolHannoverGermany

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