C5a Stimulates Eosinophil Adhesion to Human Lung Microvascular Endothelial or Epithelial Cells: Priming with TNFα or IL-5

  • Anne C. Burke-Gaffney
  • David M. T. Macari
  • Paul G. Hellewell
Part of the NATO ASI Series book series (NSSA, volume 294)

Abstract

Eosinophils are thought to play a key role in perpetuating inflammatory changes and airway epithelial damage seen in asthma. Binding of eosinophils to endothelial cell adhesion molecules facilitates the migration of these cells from blood vessels into the respiratory tract. Subsequent eosinophil adhesion to airway epithelium may contribute to epithelial damage. A knowledge of factors that may potentiate or “prime” eosinophil adhesion to endothelial or epithelial cells may have important implications for the development of new anti-asthma therapies. For example, it has been shown that transendothelial migration of eosinophils in vitro can be “primed” by cytokines such as interleukin-5 (IL-5), IL-3 or GM-CSF [1]. We have recently shown, in vivo, that tumor necrosis factor α (TNFα) causes priming of eosinophil accumulation in guinea pig skin [2]. In the present in vitro study we have investigated priming effects of TNFα or IL-3 on C5a-induced adhesion of human eosinophils to human lung microvascular endothelial cells (LMVEC) or to a lung epithelial cell line (A549), in culture.

Keywords

Airway Epithelium Lung Epithelial Cell Transendothelial Migration Human Eosinophil Lung Epithelial Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Ebisawa, M., Liu, C., Yamada, T., Kato, M., Lichtenstein, L.M. et al., (1994) J. Immunol., 152, 4590.PubMedGoogle Scholar
  2. 2.
    Macari, D.M., Teixeira, M.T. & Hellewell, P.G. (1996) Am. J. Respir. Crit. Care Med. 153, A204.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Anne C. Burke-Gaffney
    • 1
  • David M. T. Macari
    • 1
  • Paul G. Hellewell
    • 1
  1. 1.Applied Pharmacology, National Heart and Lung InstituteImperial CollegeLondonUK

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