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Induction of Cyclo-Oxygenase in Human Vessels in vitro

  • David Bishop-Bailey
  • El B. Haddad
  • Simon Larkim
  • John R. Pepper
  • Magdi Yacoub
  • Timothy J. Williams
  • Jane A. Mitchell
Part of the NATO ASI Series book series (NSSA, volume 294)

Abstract

Cyclo-oxygenase (COX) converts free arachidonic acid to prostaglandin (PG)H2, the precursor to a group of lipid mediators including prostacyclin (PGI2), PGE2, and thromboxane (TX) A2. The predominant COX metabolite released from vessels is PGI2, which is a vasodilator, and an inhibitor of cellular proliferation, platelet adhesion and activation, and cholesterol metabolism (see Willis et al., 1986; Hajjar and Pomerantz, 1992). COX is now known to exist in at least 2 different isoforms; a constitutively expressed COX-1, and a cytokine inducible COX-2 (see Mitchell et al., 1995). Within vessels COX-1 is normally the dominant isoform, and is concentrated mainly in the endothelial cell layer. The underlying vascular smooth muscle has lower COX activity, but is a rich source of PGI2 synthetase. In animal models, COX-2 is induced in the vascular smooth muscle layer after mechanical damage to the vessel (Rimarchin et al., 1994). However, the expression of COX-2 in human vessels has not been demonstrated.

Keywords

Saphenous Vein Internal Mammary Artery Endothelial Cell Layer Organ Culture System Free Arachidonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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  4. Hajjar DP and Pomerantz KB. FASEB J 1992; 6:2933–2941.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • David Bishop-Bailey
  • El B. Haddad
    • 1
  • Simon Larkim
  • John R. Pepper
    • 2
  • Magdi Yacoub
    • 3
  • Timothy J. Williams
  • Jane A. Mitchell
    • 3
  1. 1.Department of Thoracic Medicine, Department of Applied Pharmacology, The National Heart and Lung InstituteImperial CollegeLondonUK
  2. 2.Department of Cardiothoracic SurgeryRoyal Brompton HospitalLondonUK
  3. 3.Departmemt of Anaesthesia and Critical Care MedicineRoyal Brompton HospitalLondonUK

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