Physicochemical Characterization of DOTAP-Containing Lipoplexes by Fluorescent Probes: Relevance to Lipofection

  • Danielle Hirsch-Lerner
  • Nicolaas J. Zuidam
  • Yechezkel Barenholz
Chapter
Part of the NATO ASI Series book series (NSSA, volume 300)

Abstract

Delivering therapeutic genes to cells by their complexation with cationic liposomes to form lipoplexes has been shown to be efficient in vitro and in vivo (Behr, 1994; Ledley, 1995). The lipoplexes (plasmid DNA complexed with cationic liposomes) are safer than viral vectors (Mulligan, 1993; Crystal, 1995) for the following reasons: the absence of viral DNA, no constraint on DNA size, protection of DNA from degradation, and ability to target recombinant genes to specific cells. Therefore, lipoplexes might become the mainstream of research for gene therapy. The successful use of these lipoplexes will depend on their efficient delivery to cells and their ability to produce therapeutic levels of gene expression.

Keywords

Lipid Bilayer Fluorescence Anisotropy Cationic Lipid Cationic Liposome Static Light Scattering 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Danielle Hirsch-Lerner
    • 1
  • Nicolaas J. Zuidam
    • 2
  • Yechezkel Barenholz
    • 1
  1. 1.Department of BiochemistryThe Hebrew University-Hadassah, Medical SchoolJerusalemIsrael
  2. 2.Department of PharmaceuticsUtrecht UniversityUtrechtThe Netherlands

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