Abstract
The classic pseudomonads are characterized by polar flagella, ranging from a single polar flagellum, Pseudomonas aeruginosa, to several polar flagella in Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. The only exception is Burkholderia mallei, which is permanently immotile and interestingly requires a living host for survival. It is the causal agent of the disease, glanders, in horses. Several of the multipolar flagellar types have been removed from the Pseudomonas genus based on r-RNA probe comparisons (Holloway, 1996), most recently to the Burkholderia spp. Some of these groups are discussed separately for comparison. The bulk of this chapter, however, focuses on P. aeruginosa because most studies of flagella have centered on this organism, primarily because of its clinical importance, but also because of its own metabolic uniqueness and versatility.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Alam, M., and Oesterhelt, D., 1984, Morphology function and insolation of halobacteria flagella, J. Mol. Biol. 176: 459–475.
Allison, J., Dawson, M., Drake, D., and Montie, T. C., 1985, Electrophoretic separation and molecular weight characterization of Pseudomonas aeruginosa H-antigen flagellins, Infect. Immun. 49: 770–774.
Anderson, T. R., and Montie, T. C., 1989, Flagellar antibody stimulated opsono-phagocytosis of Pseudomonas aeruginosa associated with response to either a-or b-type flagellar antigen, Can. J. Microbiol. 35: 755–763.
Anderson, T. R., and Montie, T. C., 1987, Opsonophagocytosis of Pseudomonas aeruginosa treated with antiflagellar serum, Infect. Immun. 55: 3204–3206.
Anderson, T. R., Montie, T. C., Murphy, M. D., and McCarthy, V. P., 1989, Pseudomonas aeruginosa flagellar antibodies in patients with cystic flbrosis, J. Clin. Microbio. 27: 2789–2793.
Ansorg, T., 1978, Flagella specific H antigenic schema of Pseudomonas aeruginosa, Zentbl. Bakt. Mikrobiol. Hyg. 242: 228–238.
Ansorg, R., and Schmitt, W., 1980, Immunologishe and elektrophoretische Charakterisierung der flagelline unterschied licher H-typen von Pseudomonas aeruginosa, Med Microbiol. Immunol. 163: 217–226.
Arora, S. K., Titchings, B. W., Almira, E. C., Lory, S., and Ramphai, R., 1996, Cloning and characterization of Pseudomonas aeruginosa fliF, necessary for flagellar assembly and bacterial adherence to mucin, Infect. Immun. 64: 2130–2136.
Atkinson, M., Allen, C., and Sequeira, L., 1992, Tyrosine phosphorylation of a membrane protein from Pseudomonas solanacearum, J. Bacteriol. 174: 4356–4360.
Brett, P. J., Mah, D. C. W., and Woods, D. E., 1994, Isolation and characterization of Pseudomonas pseudomallei flagellin proteins, Infect. Immun. 62(5): 1914–1919.
Brickman, C. S., Kelly-Wintenberg, K., and Montie, T. C., 1997, Comparative analysis of flagellin genes of wild-type fla+ and non-motile fla— P. aeruginosa strains, In: Abstracts of the 97th General Meeting of the American Society for Microbiology, 1997, American Society for Microbiology, Washington D.C., #D-42, p. 215.
Brimer, C. D., Kelly-Wintenberg, K., and Montie, T. C., 1997, Cloning and characterization of Pseudomonas aeruginosa. a-type flagellin genes, In: Abstracts of the 97th General Meeting of the American Society for Microbiology, 1997, American Society for Microbiology, Washington, D.C., #D-43, p. 215.
Cha, H., R. Nichols, and Montie, T. C., 1996, Posttranslational phosphorylation of Pseudomonas aeruginosa by an envelope kinase in vitro, In: Abstracts of the 96th General Meeting of the American Society for Microbiology, 1996. American Society for Microbiology, Washington, D.C., D-73, p. 254.
Craven, R. C., and Montie, T. C., 1985, Effect of nitrogen source on the chemotaxis of Pseudomonas aeruginosa toward amino acids, J. Bact. 164: 544–549.
Craven, R. C., and Montie, T. C., 1981, Motility and chemotaxis of three strains of Pseudomonas aeruginosa used for virulence studies, Can. J. Microbiol. 25: 458–460.
Craven, R. C., and Montie, T. C., 1985, Effect of nitrogen source on the chemotaxis of Pseudomonas aeruginosa toward amino acids. J. Bacteriol. 164: 544–549.
Doig, P., Kinsella, N., Guerry, P., and Trust, T. J., 1996, Characterization of post-transnational modification of Campylobacter flagellin: Identification of a sero-specific gly-cosylation moiety. Mol. Microbiol. 19(2): 379–387.
Drake, D., and Montie, T. C., 1988, Flagella, motility, and invasive virulence of Pseudomonas aeruginosa, J. Gen. Microbiol. 134: 43–52.
Drake, D., and Montie, T. C., 1987, Protection against Pseudomonas aeruginosa infection by passive transfer of anti-flagellar serum, Can. J. Microbiol. 33: 755–763.
Garnak, M., and Reeves, H. C., 1978, Phosphorylation of isocitrate dehydrogenase of Escherichia coli, Science. 203: 1111–1112.
Gussin, G. N., Ronson, C. W., and Ausubel, F. M., 1986, Regulation of nitrogen fixation genes, Annu. Rev. Genet. 20: 567–591.
Holder, I. A., and Neely, A. N., 1989, Combined host and specific anti-Pseudomonas-directed therapy for Pseudomonas aeruginosa infections in burned mice: Experimental results and theoretic considerations, J. Burn Care Rehabil. 10: 131–137.
Holder, I. A., Wheeler, R., and Montie, T. C., 1982, Flagellar preparations from Pseudomonas aeruginosa animal protection studies, Infect. Immun. 35: 276–280.
Holloway, B. W., 1996, Pseudomonas genetics and taxonomy, in: Molecular Biology of Pseudomonas., (T. Nakazawa, K. Furakawa, D. Haas, and S. Silver, eds.), ASM Press, Washington, D.C., pp. 22–32.
Ishimoto, K. S., and Lory, S., 1989, Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase, Proc. Natl. Acad. Sci. USA. 86: 1954–1957.
Jarrell, K. F., Bayley, D. P., and Kostyukova, A. S., 1996, The archeal flagellum: A unique motility structure, J. Bacteriol. 178(17): 5057–5064.
Jin, S., Ishimoto, K., and Lory, S., 1994, Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa, J. Bacteriol. 176: 1313–1322.
Kelly-Wintenberg, K., Anderson, T., and Montie, T. C., 1990, Phosphorylated tyrosine in the flagellum filament of Pseudomonas aeruginosa, J. Bacteriol. 172: 5135–5139.
Kelly-Wintenberg, K., South, S., and Montie, T. C., 1993, Tyrosine phosphate in a-and b-type flagellins of Pseudomonas aeruginosa, J. Bacteriol. 175: 2458–2461.
Kelly-Wintenberg, K., and Montie, T. C., 1994, Chemotaxis to oligopeptides by Pseudomonas aeruginosa, Appl. Environ. Microbiol. 60: 363–367.
Kennedy, P. J., and Potts, M., 1996, Fancy meeting you here! A fresh look at “prokaryotic” protein phosphorylation, J. Bacteriol. 178: 4759–4764.
Kuboni, T., Shimamoto, N., Yanaguchi, S., Yanaguchi, K., and Aizawa, S., 1992, Morphological pathway of flagellar assembly in Salmonella typhimurium, J. Mol. Biol. 226: 433–446.
Landsperger, W. J., Kelly-Wintenberg, K., Montie, T. C., Knight, L. S., Hansen, M. B., Huntenburg, C. C., and Schneidkraut, M. J., 1994, Inhibition of bacterial motility with human anti-flagellar monoclonal antibodies attenuates Pseudomonas aeruginosa-in-duced pneumonia in the immunocompetent rat, Infect. Immun. 62: 4825–4830.
Landsperger, W. J., South, S. L., Kelly-Wintenberg, K., Montie, T. C., and Huntenberg, C. C., 1993, Immunoreactivity of human IgGl monoclonal antibodies with flagella of Pseudomonas aeruginosa, Abstracts ASM, E55: 152.
Lanyi, B., 1970, Serological properties of Pseudomonas aeruginosa. II. Type-specific thermo-labile (flagellar) antigens, Acta Microbiol. Acad. Sci. Hung. 17: 35–48.
Lechner, J. F., and Wieland, F., 1989, Structure and biosynthesis of prokaryotic glycopro-teins, Ann. Rev. Biochem. 58: 173–194.
Lechner, J. F., Weiland, F., and Sumper, M., 1985, Transient methylation of dilichol oligosacharides as an obligatory step in halobacterial sulfated glycoprotein biosynthesis, J. Biol. Chem. 260: 8984–8989.
Legace, J., Pelaquin, L., Kermani, P., and Montie, T. C., 1995, IgG subclass responses to Pseudomonas aeruginosa a-and b-type flagellins in patients with cystic fibrosis: A prospective study. J. Med. Microbiol. 43: 270–276.
Luzar, M. A., and Montie, T. C., 1985, A virulence and altered physiological properties of cystic fibrosis strains of Pseudomonas aeruginosa, Infect. Immun. 50: 572–576.
Luzar, M. A., Thomassen, M. J., and Montie, T. C., 1985, Flagella and motility alterations in Pseudomonas aeruginosa strains from patients with cystic fibrosis: Relationship to patient clinical condition, Infect. Immun. 50: 577–582.
Mahenthiralingam, E., Campbell, M. E., and Speert, D. P., 1994, Nonmotility and pha-gocytic resistance of Pseudomonas aeruginosa isolates from chronically colonized patients with cystic fibrosis, Infect. Immun. 62: 596–605.
Malakooti, J., Ely, B., and Matsumura, P., 1994, Molecular characterization, nucleotide sequence, and expression of the fliO, fliP, fliQ, and fliR genes of Escherichia coli, J. Bacterial 176: 189–197.
Matsumoto, A., Hong, S. K., Ishizuka, H., Horinouchi, S., and Beppu, T., 1994, Phospho-rylation of the AFSR protein involved in secondary metabolism in streptomyces species by a eucaryotic-type protein kinase. Gene 146: 47–56.
McCartney, B., Howell, L., Kennelly, P. J., and Potts, M., 1997, Protein tyrosine phospho-rylation in the cyanobacterium Anabaena sp. strain PCC 7120, J. Bacterial 179(7): 2314–2318.
McManus, A. T., Moody, E. E., and Mason, A. D., 1980, Bacterial motility: A component in experimental Pseudomonas aeruginosa burn wound sepsis, Burns 6: 235–239.
Moens, S., Michiels, K., and Vanderleyden, J., 1995, Glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense, a gram-negative nitrogen-fixing bacterium, Microbiology 141: 2651–2657.
Montie, T. C., and Anderson, T. C., 1988, Enzyme linked immunosorbent assay for detection of Pseudomonas aeruginosa H (flagellar) antigen, Eur.J. Clin. Microbiol. Infect. Dis. 7: 256–260.
Montie, T. C., Dorner, F., McDonel, J. C., and Mitterer, A., U.S. Patent 4,831,121, May 1989.
Montie, T. C., Doyle-Huntzinger, D., Craven, R. C., and Holder, F. A., 1982, Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model, Infect. Immun. 38: 1296–1298.
Montie, T. C., Drake, D., Sellin, H., Slater, O., and Edmonds, S., 1987, Motility, virulence, and protection with a flagella vaccine against Pseudomonas aeruginosa infection, In: Antibiotics and Chemotherapy (D. Doring, I. A. Holder, and K. Botzenhart, eds.), Karger, Basel, pp. 233–248.
Montie, T. C., Philips, D., and Landsperger, W., 1997, Characterization of monoclonal antibodies to Pseudomonas aeruginosa type-A flagellar antigen, Behring Inst. Mitt. 98: 424–433.
Montie, T. C., and Stover, G. B., 1983, Flagellar preparations from Pseudomonas species: Isolation and characterization by molecular weight, J. Clin. Microbiol 18: 452–456.
Moulton, R. C., and Montie, T. C., 1979, Chemotaxis by Pseudomonas aeruginosa, J. Bacteriol. 137: 274–280.
Ochi, H., Ohtsuka, H., Yokota, S., Uezumi, I., Terashima, M., Irie, K., and Noguchi, H., 1991, Inhibitory activity on bacterial motility and in vivo protective activity of human monoclonal antibodies against flagella of Pseudomonas aeruginosa, Infect Immun. 59(2): 550–554.
Ohtake, H., Kato, J., Kuroda, A., Taguchi, K., and Sakai, Y., 1996, Chemotactic signal transduction network in Pseudomonas aeruginosa, in: Molecular Biology of Pseudomonads (T. Nadazawa, K. Furakawa, D. Haas, S. Silver, eds.), ASM Press, Washington, D.C., pp. 188–194.
Oishi, K., Sonoda, F., Iwagaki, A., Penglertnapagorn, P., Watanabe, K., Nagatake, T., Siadiak, A., Pollack, M., and Matsomoto, K., 1993, Therapeutic effects of human antiflagella monoclonal antibody in a neutropenic murine model of Pseudomonas aeruginosa pneumonia, Antimicrob. Agents Chemother. 37: 164–170.
Poole, K., and Hancock, R. E. W., 1983, Secretion of alkaline phosphatase and phospholipase C is specific and does not involve an increase in outer membrane permeability. FEMS Microbiol. Lett. 16: 25–29.
Ranakrishran, F., Zhao, J-L., and Newton, A., 1994, Multiple stimulational proteins are required for both transcriptional activation and flagellar genes, J. Bacteriol 176: 7587–7600.
Ray, M. K., Kumar, G. S., and Shivaji, S., 1994, Tyrosine phosphorylation of a cytoplasmic protein from the Antarctic psychrotrophic bacterium Pseudomonas syringae, FEMS Microbiol. Lett. 122: 49–54.
Ritchings, B. W., Almira, E. C., Lory, S., and Ramphal, R., 1995, Cloning and phenotypic characterization of fleS and fleR, new response regulators of Pseudomonas aeruginosar which regulate motility and adhesion to mucin, Infect. Immun. 63: 4868–4876.
Rosok, M. J., Stebbins, M. R., Connelly, K., Lostrom, M. E., and Sidiak, A. W., 1990, Generation and characterization of murine antiflagellum monoclonal antibodies that are protective against lethal challenge with Pseudomonas aeruginosa, Infect. Immun. 58: 3819–3828.
Rotering, H., and Dorner, F., 1989, Studies on a Pseudomonas aeruginosa flagella vaccine, in: Antibiotics and Chemotherapy, (N. Hoiby, S. S. Pederson, G. H. Shanel, G. Doring, and I. A. Holder, eds.), Karger, Basel, Vol. 42, pp. 218–228.
Simpson, D. A., Ramphal, R., and Lory, S., 1995. Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence, Infect. Immun. 63: 2950–2957.
Smith, S. C., Kennelly, P. J., and Potts, M., 1997, Protein tyrosine phosphorylation in the archaea, J. Bacteriol. 179: 2418–2420.
South, S., Nichols, R., and Montie, T. C., 1994, Tyrosine kinase activity in Pseudomonas aeruginosa, Mol. Microbiol. 12: 903–910.
Southam, G., Kalmokoff, M. L., Jarrell, K. F., Koval, S. F., and Beveridge, T. J., 1990, Isolation, characterization, and cellular insertion of the flagella from two strains of the archaeabacterium Methanospirillum hungatei, J. Bacteriol. 176(6): 3221–3228.
Spangenberg, C., Heuer, T., Burger, C., and Tummler, B., 1996, Genetic diversity of flagellins of Pseudomonas aeruginosa, FEBS Lett. 396(2-3): 213–217.
Starnbach, M. N., and Lory, S., 1992, The fliA (rpoF) gene of Pseudomonas aeruginosa encodes an alternative sigma factor required for flagellin syntheses, Mol. Microbiol. 6(4): 459–469.
Suzuki, T., and Iino, T., 1980, Isolation and characterization of multiflagellate mutants of Pseudomonas aeruginosa, J. Bacteriol. 143: 1471–1479.
Thony, B., and Hennecke, H., 1989, The-24/-12 promoter comes of age, FEMS Microbiol. 5: 341–357.
Totten, P. A., Lara, J. C., and Lory, S., 1990, The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene, J. Bacteriol. 172: 389–396.
Totten, P. A., and Lory, S., 1990, Characterization of the type a flagellin gene from Pseudomonas aeruginosa PAK, J. Bacteriol. 172: 7188–7199.
Tsuda, N., and Iino, T., 1983a, Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501, J. Bacteriol. 153: 1008–1017.
Tsuda, N., and Iino, T., 1983b, Transductional analysis of the flagellar genes in Pseudomonas aeruginosa, J. Bacteriol. 153: 1018–1926.
Virjii, M., Saunders, J. R., Siims, G., Makepeace, K., Maskell, D., and Fergeson, D. J., 1993, Pilus-facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: Modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin, Mol. Microbiol. 10: 1013–1028.
Wang, J. Y., and Koshland, D. E., Jr., 1978, Evidence for protein kinase activities in the prokaryote Salmonella typhimurium, J. Biol. Chem. 253: 7605–7608.
Whiteside, T. M., and Rhodes-Roberts, M. E., 1985, Biochemical and serological properties of purified flagella and flagellins of some Pseudomonas spp, J. Gen. Microbiol. 131: 873–883.
Winstanley, C., Coulson, M. A., Wepner, B., Morgan, J. A. W., and Hart, C. A., 1996, Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa, Microbiology 142: 2145–2151.
Winstanley, C., Morgan, J. A. W., Pickup, R. W., and Saunders, J. R., 1994, Molecular cloning of two Pseudomonas flagellin genes and basal body structural genes, Microbiology 140: 2019–2031.
Xiao-Song, H. E., Rivkina, M., Stocker, B. A. D., and Robinson, W. S., 1994, Hypervariable region IV of Salmonella fliC encodes a dominant surface epitope and a stabilizing factor for functional flagella, J. Bacteriol. 176: 2406–2414.
Zhang, C-C., 1996, Bacterial signaling involving eukaryotic-type kinases, Mol. Microbiol. 20(1): 9–15.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Montie, T.C. (1998). The Flagellum. In: Montie, T.C. (eds) Pseudomonas. Biotechnology Handbooks, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0120-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0120-0_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0122-4
Online ISBN: 978-1-4899-0120-0
eBook Packages: Springer Book Archive