Taurine 3 pp 431-439 | Cite as

Unique Pharmacological Interactions of Taurine and Chelerythrine in the Retina

  • Julius D. Militante
  • John B. Lombardini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)


The effects of taurine and chelerythrine (CHT) on ATP-dependent calcium uptake and the phosphorylation of the ~20 kDa phosphoprotein in the retina were compared. In the absence of the CHT, taurine stimulated ATP-dependent calcium uptake and attenuated the phosphorylation of the ~20 kDa phosphoprotein. On the other hand, CHT produced the opposite results in the absence of taurine. When the two agents were used simultaneously, it was found that CHT non-competitively inhibited the action of taurine to stimulate calcium uptake, while taurine non-competitively inhibited the action of CHT to stimulate the phosphorylation of the ~20 kDa phosphoprotein. The data present an unusual pharmacological mechanism for controlling the signal transduction pathway involving the two distinct cellular processes being studied. Given the unique data, a control system is proposed in which the function of the ~20 kDa phosphoprotein is linked to the stimulation of ATP-dependent calcium uptake.


Calcium Uptake Okadaic Acid Taurine Depletion Chelerythrine Chloride Benzophenanthridine Alkaloid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Baylor, D., 1996, How photons start vision, Proc. Natl. Acad. Sci., 93:560–565.PubMedCrossRefGoogle Scholar
  2. 2.
    Cohen, H.G., Seifen, E.E., Straub, K.D., Tiefenback, C., and Stermitz, F.R., 1978, Structural specificity of the NaK-ATPase inhibition by sanguinarine, an isoquinoline benzophenanthridine alkaloid, Biochem. Pharmacol., 27:2555–2558.PubMedCrossRefGoogle Scholar
  3. 3.
    Herbert, J.M., Augereau, J.M., Gleye, J., and Maffrand, J.P., 1990, Chelerythrine is a potent and specific inhibitor of protein kinase C, Biochem. Biophys. Res. Comm., 172:993–999.PubMedCrossRefGoogle Scholar
  4. 4.
    Koch K.-W., 1995, Control of photoreceptor proteins by Ca2+, Cell Calcium, 18:314–321.PubMedCrossRefGoogle Scholar
  5. 5.
    Kuo, C.-H. and Miki, N., 1980, Stimulatory effect of taurine on Ca-uptake by disk membranes, Biochem. Biophys. Res. Comm., 94:646–651.PubMedCrossRefGoogle Scholar
  6. 6.
    Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacterio-phage T4, Nature, 227:680–685.PubMedCrossRefGoogle Scholar
  7. 7.
    Lombardini, J.B., 1985, Effects of taurine on calcium ion uptake and protein phosphorylation in rat retinal membrane preparations, J. Neurochem., 45:268–275.PubMedCrossRefGoogle Scholar
  8. 8.
    Lombardini, J.B., 1991, Taurine: retinal function, Brain Res. Rev., 16:151–169.PubMedCrossRefGoogle Scholar
  9. 9.
    Lombardini, J.B., 1992, Effects of taurine on the phosphorylation of specific proteins in subcellular fractions of the rat retina, Neurochem. Res., 17(8):821–824.PubMedCrossRefGoogle Scholar
  10. 10.
    Lombardini, J.B., 1993, Partial characterization of an ~20K Mrretinal protein whose phosphorylation is inhibited by taurine, Biochem. Pharmacol, 46:1445–1451.PubMedCrossRefGoogle Scholar
  11. 11.
    Lombardini, J.B., 1993, The inhibitory effects of taurine on protein phosphorylation: comparison of various characteristics of the taurine-affected phosphoproteins present in rat retina, brain and heart, Adv. Exp. Med. Bio., 359:9–17.Google Scholar
  12. 12.
    Lombardini, J.B., 1995, Paradoxical stimulatory effect of the kinase inhibitor chelerythrine on the phosphorylation of a ~20K M protein present in the mitochondrial fraction of rat retina, Brain Res., 673:194–198.PubMedCrossRefGoogle Scholar
  13. 13.
    Lombardini, J.B., Liebowitz, S.M., and Chou, T.-C., 1989, Analogues of taurine as stimulators and inhibitors of ATP-dependent calcium ion uptake in rat retina: combination kinetics, Mol. Pharmacol., 36:256–264.PubMedGoogle Scholar
  14. 14.
    Lombardini, J.B., Young, R.S.L., and Props, C.L, 1996, Taurine depletion increases phosphorylation of a specific protein in the rat retina, Amino Acids, 10:153–165.CrossRefGoogle Scholar
  15. 15.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193:265–275.PubMedGoogle Scholar
  16. 16.
    Militante, J.D. and Lombardini, J.B., 1997, The effect of taurine on chelerythrine inhibition of calcium uptake and ATPase activity in the rat retina, Biochem. Pharmacol., submitted for publication.Google Scholar
  17. 17.
    Morrison, J.F., Ennor, A.H., and Griffiths, D.E., 1958, The preparation of barium monophosphotauro-cyamine, Biochem. J., 68:447–452.PubMedGoogle Scholar
  18. 18.
    Nakanishi, S., Matsuda, Y., Iwahashi, K., and Kase, H., 1986, K-252b, c and d, potent inhibitors of protein kinase C from microbial origin, J. Antibiotics, 39:1066–1071.CrossRefGoogle Scholar
  19. 19.
    Ottlecz, A., Garcia, C.A., Eichberg, J., and Fox, D.A., 1993, Alterations in retinal Na+, K+-ATPase in diabetes: streptozotocin-induced and Zucker diabetic fatty rats, Curr. Eye Res. 12:1111–1121.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Julius D. Militante
    • 1
  • John B. Lombardini
    • 1
    • 2
  1. 1.Departments of PharmacologyTexas Tech University Health Sciences CenterLubbockUSA
  2. 2.Ophthalmology & Visual SciencesTexas Tech University Health Sciences CenterLubbockUSA

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