Taurine 3 pp 255-260 | Cite as

The Anion-Exchanger AE1 is a Diffusion Pathway for Taurine Transport in Rat Erythrocytes

  • Rafael Martín del Río
  • José M. Solís
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)

Abstract

A putative osmolyte is required to meet two main criteria: to be highly concentrated inside the cell, and to possess a specific pathway across the plasma membrane allowing for its osmotic efflux12. In the case of taurine, an admitted osmolyte in several animal species, high intracellular levels are maintained in most mammalian tissues by the action of well characterized sodium/chloride-dependent carriers14,20,24,25. The nature of the taurine efflux carrier, however, remains to be assessed, although it has been demonstrated in several cell types11,17,22 that it consists of a diffusion pathway with pharmacological properties similar to a chloride channel. An alternative pathway for taurine exit has been proposed based on extensive studies with red blood cells (RBCs). This release occurs through a transport system that is sensitive to compounds that are well-known inhibitors of the RBC’s anion-exchanger (AE1)8. It has been claimed, therefore, that taurine uses this diffusion transport system to exit the erythrocyte9 (but see16). This assumption is supported by the fact that some N-derivative analogs of taurine such as, NBD-taurine, Cl-taurine, NAP-taurine or NIP-taurine, are either substrates and/or competitive inhibitors of this anion-exchanger1. In addition, AE1 transports many different organic compounds, including some amino acids such as glycine15.

Keywords

Chloride Channel Regulatory Volume Decrease Diffusion Pathway Taurine Transport Taurine Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Cabantchik, Z.I. and Greger, R., 1992, Chemical probes for anion transporters of mammalian cell membranes, Am. J. Physiol. 262:C803–827.PubMedGoogle Scholar
  2. 2.
    Cabantchik, Z.I., Baishin, M., Breuer, W., and Rothstein, A., 1975, Pyridoxal phosphate. An anionic probe for protein amino groups exposed on the outer and inner surfaces of intact human red blood cells, J. Biol. Chem., 250:5130–5136.PubMedGoogle Scholar
  3. 3.
    Cousin, J.L. and Motais, R., 1982, Inhibition of anion transport in the red blood cell by anionic amphiphilic compounds. I. Determination of the flufenamate-binding site by proteolytic dissection of the band 3 protein, Biochim. Biophys. Acta., 687:147–155.PubMedCrossRefGoogle Scholar
  4. 4.
    Eidelman, O., Yanai, P., Englert, H.C., Lang, H.G., Greger, R., and Cabantchik, Z.I., 1991 Macro-molecular conjugates of transport inhibitors: new tools probing topography of anion transport proteins, Am. J. Physiol., 260:C1094–C1103.PubMedGoogle Scholar
  5. 5.
    Fugelli, K. and Thoroed, S.M., 1986, Taurine transport associated with cell volume regulation in flounder erythrocytes under unisosmotic conditions, J. Physiol., 374:245–261.PubMedGoogle Scholar
  6. 6.
    Garcia-Romeu, F., Cossins, A.R., and Motais, R., 1991, Cell volume regulation by trout erythrocytes: Characteristics of the transport systems activated by hypotonic swelling, J.Physiol., 440:547–567.PubMedGoogle Scholar
  7. 7.
    Glibowicka, M., Winckler, B., Aranibar, N., Schuster, M., Hanssum, H., Rüterjans, H., and Passow H., 1988, Temeperature dependece of anion transport in the human red blood cell, Biochim. Biophys. Acta., 946:345–358.PubMedCrossRefGoogle Scholar
  8. 8.
    Goldstein, L., Brill, S.R., and Freund, E.V., 1990, Activation of taurine efflux in hypotonically stressed elasmobranch cells: inhibition by stilbene disulfonates, J. Exp. Zool., 254:114–118.CrossRefGoogle Scholar
  9. 9.
    Goldstein, L. and Brill, S.R., 1991, Volume-activated taurine efflux from skate erythrocytes: possible band 3 involvement, Am. J. Physiol. 260:R1014–1020.PubMedGoogle Scholar
  10. 10.
    Greger, R., 1990, Chloride channel blockers, in Methods in Enzymology, Fleischer, B. and Fleischer, S., eds., Academic Press, Orlando, FL., Vol. 191, pp. 793–810.Google Scholar
  11. 11.
    Hall, J.A., Kirk, J., Potts, J.R., Rae, C., and Kirk, K., 1996, Anion channel blockers inhibit swelling activated anion cation, and nonelectrolyte transport in HeLa cells, Am. J. Physiol., 271:C579–C588.PubMedGoogle Scholar
  12. 12.
    Hoffmann, E.K. and Simonsen, L.O., 1989, Membrane mechanisms in volume and pH regulation in vertebrate cells, Physiol. Rev., 69:315–382.PubMedGoogle Scholar
  13. 13.
    Huxtable, R.J., 1989, Taurine in the central nervous system and the mammalian actions of taurine, Prog. Neurobiol, 32:471–533.PubMedCrossRefGoogle Scholar
  14. 14.
    Jhiang, S.M., Fithian, L., Smanik, P., McGill, J., Tong, Q., and Mazzaferri, E.L., 1993, Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells, FEBS Lett., 318:139–144.PubMedCrossRefGoogle Scholar
  15. 15.
    King, A.P. and Gunn, R.B., 1991, Glycine transport by human red blood cells and ghosts: evidence for glycine anion and proton cotransport by band 3, Am. J. Physiol., 261:C814–C821.PubMedGoogle Scholar
  16. 16.
    Kirk, K., Ellory, J.C., and Young, J.D., 1992, Transport of organic substrates via a volume-activated channel, J. Biol. Chem., 267:23475–23478.PubMedGoogle Scholar
  17. 17.
    Lambert, I.H. and Hoffmann, E.K., 1994, Cell swelling activates separate taurine and chloride channels in Ehrlich mouse ascites tumor cells, J. Membrane Biol., 142:289–298.CrossRefGoogle Scholar
  18. 18.
    Legrum, B. and Passow, H., 1989, Inhibition of inorganic transport across the human red blood cell membrane by chloride-dependent association of dipyridemole with a stilbene disulfonate binding site on the band 3 protein, Biochim. Biophys. Acta, 979:193–207.PubMedCrossRefGoogle Scholar
  19. 19.
    Lerma, J., Herranz, A.S., Herreras, O., Abraira, V., and Martín el Río, R., 1986, “In vivo” determination of extracellular concentration of amino acids in the rat hippocampus. A method based on brain dialysis and computerized analysis, Brain Res., 384:145–155.PubMedCrossRefGoogle Scholar
  20. 20.
    Liu, QR., López-Corcuera, B., Nelson, H., Mandiyan, S., and Nelson, N., 1992, Cloning and expression of a cDNA encoding the transporter of taurine and ²-alanine in mouse brain, P. Nat. Acad. Sci., 89:12145–12149.CrossRefGoogle Scholar
  21. 21.
    Martín del Río, R., Galarreta, M., Menéndez, N., Conejero, C., and Solís J.M., 1996, Taurine is a substrate of the anion-exchanger transport systems, in Taurine 2: Basic and Clinical Aspects, Huxtable, R., Azuma, J., Kuriyama, K., Nakagawa, M., and Baba, A., eds., Plenum Press, New York, pp. 401–407.Google Scholar
  22. 22.
    Pasantes-Morales, H., Chacón, E., Murray, R.A., and Morán, J., 1994, Properties of osmolyte fluxes activated during regulatory volume decrease in cultured cerebellar granule neurons, J. Neurosci. Res., 37:720–727.PubMedCrossRefGoogle Scholar
  23. 23.
    Passow, H., 1986, Molecular aspects of band 3 protein-mediated anion transport across the red blood cell membrane, Rev. Physiol. Bioch., 103:61–203.Google Scholar
  24. 24.
    Smith, K.E., Borden, L.A., Wang, H.D., Hartig, P.R., Branchek, T.A., and Weinshank, R.L., 1992, Cloning and expression of a high affinity taurine transporter from rat brain, Mol. Pharmacol., 42:563–569.PubMedGoogle Scholar
  25. 25.
    Uchida, S., Moo Kwon, H., Yamauchi, A., Preston, A.S., Marumo, F., and Handler, J., 1993, Molecular cloning of the cDNA for an MDCK cell Na+ and Cl--dependent taurine transporter that is regulated by hypertonicity, P. Nat. Acad. Sci., 89:8230–8234.CrossRefGoogle Scholar
  26. 26.
    Wieth, J.O., Andersen, O.S., Brahm, J., Bjerrum, P.J., and Borders, C.L., 1982, Chloride-Bicarbonate exchange in red blood cells: Physiology of transport and chemical modification of binding sites, Philos. T. Roy. Soc. London. B, 299:383–399.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Rafael Martín del Río
    • 1
  • José M. Solís
    • 1
  1. 1.Servicio de Neurobiología Departamento de InvestigaciónHospital Ramón y CajalMadridSpain

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