Taurine Chloramine Inhibits the Production of Superoxide Anion, IL-6 and IL-8 in Activated Human Polymorphonuclear Leukocytes
Polymorphonuclear leukocytes (PMN) are the initial cells recruited to the site of inflammation where foreign invaders such as microorganisms, toxic gases and chemicals elicit an inflammation reaction4,6,12. Activated PMN produce various oxygen and hydroxyl radicals which kill bacteria and fungi but may also induce indiscriminate cellular damage14. For example, HOCl/OCl- produced by the halide-dependent myeloperoxidase of PMN is highly toxic. Taurine attenuates the damage caused by HOCl/OCl- by forming taurine chloramine (Tau-Cl), a relatively non-toxic and long-lived oxidant7,9,13,15. Previously we demonstrated that Tau-Cl suppressed the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) in activated murine peritoneal macrophages and/or RAW 264.7, a murine macrophage cell line8,10,11. Tau-Cl also inhibits the production of superoxide anion in activated murine peritoneal PMN5. Since human PMN produce Tau-Cl by halide-dependent myeloperoxidase, we investigated the effect of Tau-Cl on the production of various cytokines and superoxide anion by activated human PMN. In this report we demonstrate that Tau-Cl down-regulates the production of superoxide anion, IL-6 and IL-8 by activated human PMN.
KeywordsNitric Oxide Superoxide Anion Production Phorbol Myristate Acetate Murine Peritoneal Macrophage Human Polymorphonuclear Leukocyte
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