Evaluation of Xenoestrogenic Effects in Fish on Different Organization Levels

  • Betting Schrag
  • Uwe Ensenbach
  • José Maria Navas
  • Helmut Segner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 444)


Chemicals with estrogen-like activity have drawn increasing attention during recent years because of the possible consequences for human and wildlife development and reproduction. Among wildlife species, research has been focused on animals from aquatic habitats receiving sewage and industrial effluents and agricultural runoff. For teleost fish, there exist a number of both laboratory and field reports on adverse health effects related to or induced by exposure to xenoestrogens (e. g. van der Kraak et al., 1992; Flouriot et al., 1995; Sumpter, 1995; Folmar et al., 1996; Nimrod and Benson, 1996).


Estrogen Receptor Rainbow Trout Spotted Seatrout Trout Hepatocyte Estrogenic Potency 
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  1. Campbell, P.M., Pottinger, T.G., and Sumpter, J.P., 1994, Changes in the affinity of estrogen and androgen receptors accompany changes in receptor abundance in brown and rainbow trout. Gen. Comp. Endocr. 94: 329–340.PubMedCrossRefGoogle Scholar
  2. Ensenbach, U. and Nagel, R. 1997, Toxicity of binary chemical mixtures: effects on reproduction of zebrafish. Arch. Environ. Con. Tox.32(2): 204–210.CrossRefGoogle Scholar
  3. Flouriot, G., Pakdel, F., Ducouret, B., and Valotaire, Y., 1995, Influence of xenobiotics on rainbow trout liver estrogen receptor and vitellogenin gene expression. J. Mol. Endocrinol. 15: 143–151.PubMedCrossRefGoogle Scholar
  4. Folmar, L.C., Denslow, N.D., Vijayasri, R. Chow, M., Crain, D.A., Enblom, J. Marcino, J., and Guillette, Jr, L.J., 1996, Vitellogenin induction and reduced serum testosterone concentrations in feral male carp (Cyprinus carpio) captured near a major metropolitan sewage treatment plant. Environ. Health Perspect. 104: 1096–1101.PubMedCrossRefGoogle Scholar
  5. Gillesby, B.E.; and Zacharewski, T., 1998, Exoestrogens: mechanisms of action and strategies for identification and assessment, Environ. Toxicol. Chem. 17(1): 3–14.CrossRefGoogle Scholar
  6. Jobling, S., and Sumpter, J.P., 1993, Deteregent components in sewage effluent are weakly oestrogenic to fish: an in vitro study using rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat. Toxicol. 27: 361–372.CrossRefGoogle Scholar
  7. Kavlock, J.R., Daston, G.P., DeRosa, C., Fenner-Crisp, P., Earl Grey, L., Kaattari, S., Lucier, G., Luster, M., Mac, M.J., Maczka, C., Miller, R., Moore, J., Rolland, R., Scott, G., Sheehan, D.M., Sinks, T., and Tilson, H.T., 1996, Research needs for the risk assessment of health and environmental effects of endocrine disruptors: a report of the U.S. EPA-sponsored workshop. Environ. Health Perspect. 104 (Suppl. 4): 715–740.PubMedGoogle Scholar
  8. Maitre, J.L., Valotaire, Y., and Guguen-Guillouzo, C., 1986, Estradiol-17β stimulation of vitellogenin synthesis in primary cultures of male trout hepatocytes. In Vitro Cell. Dev. Biol. 22: 337–343.CrossRefGoogle Scholar
  9. Nimrod, A.C., and Benson, W.H., 1996, Estrogenic response to xenobiotics in Channel catfish (Ictalurus punctatus), Mar. Environ. Res. 42 (1-4): 155–160.CrossRefGoogle Scholar
  10. Pellissero, C., Flouriot, G., Foucher, J.L., Bennetau, B., Dunogues, J., Le Gac, F. and Sumpter, J.P., 1993, Vitellogenin synthesis in cultured hepatocytes; an in vitro test for the estrogenic potency of chemicals. J. Steroid Biochem. Molec. Biol. 44 (3): 263–272.CrossRefGoogle Scholar
  11. Peyon, P., Baloche, S., and Burzawa-Gerard, E., 1996, Potentiating effect of growth hormone on vitellogenin synthesis induced by 17β-estradiol in primary culture of female silver eel (Anguilla anguilla L.) hepatocytes. Gen. Comp. Endocr. 102: 263–273.PubMedCrossRefGoogle Scholar
  12. Pottinger, T.G., 1986, Estrogen-binding sites in the liver of sexually mature male and female brown trout, Salmo trutta. Gen. Comp-. Endocrinol. 61: 120–126.CrossRefGoogle Scholar
  13. Segner, H., Blair, J.B., Wirtz, G., and Miller, M.R., 1994, Cultured trout liver cells: utilization of substrates and response to hormones. In Vitro Cell. Dev. Biol. 30A: 306–311.CrossRefGoogle Scholar
  14. Sumpter, J.P., 1995; Feminized responses in fish to environmental estrogens. Toxicol. Let. 82/83: 734–742.Google Scholar
  15. Sumpter, J.P., and Jobling, S. 1995, Vitellogenesis as a biomarker for oestrogenic contamination of the aquatic environment. Environ. Health Perspect. 103: 173–178.PubMedGoogle Scholar
  16. Thomas, P., and Smith, J., 1993, Binding of xenobiotics to the estrogen receptor of spotted seatrout: a screning assay for potential estrogenic effects. Mar. Environ. Res.35, 147–151.CrossRefGoogle Scholar
  17. Tsai, M.J., and O’Malley, W.O., 1994, Molecular mechanisms of action of steroid/thyroid receptor superfamily members. Annu. Rev. Biochem. 63: 451–486.PubMedCrossRefGoogle Scholar
  18. van der Kraak, G.J., Munkittrick, K.R., McMaster, M.E., Port, C.B., and Chang, J.B., 1992, Exposure to bleached kraft pulp mill effluent disrupts the pituitary-gonadal axis of white sucker at multiple sites. Toxicol. Appl. Pharmacol. 115: 224–233.PubMedCrossRefGoogle Scholar
  19. Wallace; R.A., 1985, Vitellogenesis and oocyte growth: non-mammalian vertebrates, in: Developmental biology, Browder, L., ed., Vol. 1. Plenum Press. New York., 127–177.Google Scholar
  20. Zacharewski, T., 1997, In vitro bioassays for assessing estrogenic substances, Environ. Sci. Technol. 31(3): 613–623.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Betting Schrag
    • 1
  • Uwe Ensenbach
    • 1
  • José Maria Navas
    • 1
  • Helmut Segner
    • 1
  1. 1.Dept. of Chemical EcotoxicologyCentre for Environmental ResearchLeipzigGermany

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