Molecular Cloning and Developmental Pattern of Expression of MSJ-1, a New Male Germ Cell-Specific DnaJ Homologue

  • Giovanna Berruti
  • Lucia Perego
  • Enzo Martegani
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 444)

Abstract

A cDNA encoding a new member of the DnaJ protein family has been isolated by screening a mouse testicular expression library. The predicted protein, named MSJ-1, is 242 amino acid residues-long, containing the fingerprinting J domain in the NH2 terminus. A wide tissutal Northern blot analysis reveals that MSJ-1 is expressed only in the testis, while in situ hybridization analyses demonstrate that the mRNA is first transcribed in spermatids. The antiserum developed against a MSJ-1/GST fusion protein recognizes a protein of 30 kDa in germ cell protein extracts.

Keywords

Germ Cell Acrosome Reaction Male Germ Cell Seminiferous Epithelium Male Germ Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Giovanna Berruti
    • 1
  • Lucia Perego
    • 1
  • Enzo Martegani
    • 2
  1. 1.Department of BiologyUniversity of MilanMilanoItaly
  2. 2.Dept. Physiology and BiochemistryUniversity of MilanMilanoItaly

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