Molecular Cloning and Developmental Pattern of Expression of MSJ-1, a New Male Germ Cell-Specific DnaJ Homologue
A cDNA encoding a new member of the DnaJ protein family has been isolated by screening a mouse testicular expression library. The predicted protein, named MSJ-1, is 242 amino acid residues-long, containing the fingerprinting J domain in the NH2 terminus. A wide tissutal Northern blot analysis reveals that MSJ-1 is expressed only in the testis, while in situ hybridization analyses demonstrate that the mRNA is first transcribed in spermatids. The antiserum developed against a MSJ-1/GST fusion protein recognizes a protein of 30 kDa in germ cell protein extracts.
KeywordsGerm Cell Acrosome Reaction Male Germ Cell Seminiferous Epithelium Male Germ Line
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