Abstract
The availability of cloned mycobacterial genes and suitable vectors for expression in mammalian cells has now opened a new avenue in which individual mycobacterial protein antigens can be tested for their ability to confer protective immunity. Expression of protection against mycobacteria is largely a function of antigen specific CD4 and cytotoxic CD8 T cells. We have found that intramuscular injection of plasmid DNA expressing several mycobacterial immunodominant genes can induce both T cell populations and confer protection equivalent to BCG, suggesting that this approach may lead to a new vaccine.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Barry M. A., Lai W. C., Johnston S.A., 1995, Protection against mycoplasma infection using expression library immunization. Nature, 377: 632.
Freeman, G. J., Gray, G. S., Gimmi, C. D., Lombard, D. B., Zou, L. White, M., Fingeroth, J. D., Gribben, J. G., and Nadler, L. M., 1991, Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7. J. Exp. Med., 174: 625.
Gautier, C., Mehtali, M. and Lathe, R., 1989, A ubiquitous mammalian expression vector, pHMG based on a housekeeping gene promoter. Nucleic Acids Res., 17: 8389.
Matzinger P., 1991, The JAM test a simple assay for DNA fragmentation and cell death. J.Immunol.Methods., 145: 185.
Silva, C.L. and Lowrie, D.B., 1994, A single mycobacterial protein (hsp65) expressed by a transgenic antigen-resenting cell vaccinates mice against tuberculosis. Immunol., 82: 244.
Silva, C.L., Silva, M.F., Pietro, R.C.L.R. and Lowrie, D.B. 1994, Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65. Immunol., 83: 341.
Sorensen, A.L., Nagai, S., Houen, G., Andersen, P. and Andersen, A.B., 1995, Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect. Immun., 63: 1710.
Tascon, R.E., Colston, M. J., Ragno S., Stavropoulos, E., Gregory D., Lowrie D.B., 1996, Vaccination against tuberculosis by DNA injection. Nature Medicine, 2: 888.
Taswell, C., 1981, Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J. Immunol., 126: 1614.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Tascon, R.E., Colston, M.J., Stavropoulos, E., Ragno, S., Gregory, D., Lowrie, D.B. (1997). Protection Against Tuberculosis by Plasmid DNA. In: Gregoriadis, G., McCormack, B., Allison, A.C. (eds) Vaccine Design. NATO ASI Series, vol 293. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0062-3_18
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0062-3_18
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0064-7
Online ISBN: 978-1-4899-0062-3
eBook Packages: Springer Book Archive