Binding of Daunorubicin and Doxorubicin to Erythrocytes Treated with Glutaraldehyde
Intact human erythrocytes can bind anthracycline antibiotics (daunorubicin and doxorubicin) during incubation in the isotonic medium containing the antibiotic. Bound antibiotics can be immobilized in the erythrocytes using glutaraldehyde treatment. It was found, however, that erythrocytes pretreated with glutaraldehyde can uptake daunorubicin and doxorubicin from the incubation medium and do it faster than untreated cells. The antibiotics are readily released from these erythrocytes in the antibiotic-free medium. Our findings provide evidence that immobilization of anthracycline antibiotics in the erythrocytes using glutaraldehyde treatment is connected with the chemical binding of the antibiotics to different erythrocyte components.
KeywordsIncubation Medium Human Erythrocyte Cytosine Arabinoside Anthracycline Antibiotic Isotonic Medium
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