Rat Carrier Erythrocytes Circulate and Arrive to Organs

  • F. J. Alvarez
  • J. A. Jordán
  • J. C. Murciano
  • J. Luque
  • A. Herráez
  • J. C. Díez
  • M. C. Tejedor


Different delivery systems are currently used in therapy. They have the advantage of protecting the active substance from rapid clearance and avoiding toxic side effects. Among the many carrier systems proposed12, RBCs have many desirable properties: they are naturally biodegradable and may stay in circulation over prolonged periods of time11,25; RBCs are easily obtainable and large amounts of material can be entrapped in a small volume of cells by hypotonic dialysis; autologous cells elicit little or no immune response7,16,20.


Carbonic Anhydrase Human Erythrocyte Encapsulation Process Sodium Chromate Organ Uptake 
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  1. 1.
    F.J. Alvarez, A. Herráez, J.C. Murciano, J. A. Jordán, J.C. Díez, and M.C. Tejedor, In vivo survival and organ uptake of loaded carrier rat erythrocytes. J. Biochem. 120:286 (1996).PubMedCrossRefGoogle Scholar
  2. 2.
    F.J. Alvarez, A. Herráez, and M.C. Tejedor, Fluorescence analysis of carrier rat and human erythrocytes loaded with FITC-dextran. Cytometry 24:181 (1996).PubMedCrossRefGoogle Scholar
  3. 3.
    F.J. Alvarez, A. Herráez, M.C. Tejedor, and J.C. Díez, Behaviour of isolated rat and human red blood cells upon hypotonic-dialysis encapsulation of carbonic anhydrase and dextran. Biotechnol. Appl. Biochem. 23:173(1996).PubMedGoogle Scholar
  4. 4.
    U. Benatti, E. Zocchi, M. Tonetti, L. Guida, C. Polvani, and A. De Flora, Enhanced antitumor activity of adriamycin by encapsulation in mouse erythrocytes targeted to liver and lungs. Pharmacol. Res. 21:27 (1989).PubMedCrossRefGoogle Scholar
  5. 5.
    L. Chiarantini, A. Antonelli, G. Serafini, A. Fraternale, and M. Magnani, In vivo survival and in vitro phagocytosis of engineered erythrocytes. Adv. Biosci. 92:83 (1994).Google Scholar
  6. 6.
    L. Chiarantini, J. Johnson, and J.R. DeLoach, Optimized recirculation survival of mouse carrier erythrocytes. Blood Cells 17:607 (1991).PubMedGoogle Scholar
  7. 7.
    A. De Flora, E. Zocchi, L. Guida, C. Polvani, and U. Benatti, Conversion of encapsulated 5-fluoro-2′-deoxyuridine 5′-monophosphate to the antineoplastic drug 5-fluoro-2′-deoxyuridine in human erythrocytes. Proc. Nat. Acad. Sci. USA 85:3145 (1988).PubMedCrossRefGoogle Scholar
  8. 8.
    J.R. DeLoach, K. Andrews, W. Satterfield, and M. Keeling, Intraperitoneal administration of carrier erythocytes in dogs: an improved method for delivery of L-asparaginase. Biotechnol. Appl. Biochem. 12:331 (1990).PubMedGoogle Scholar
  9. 9.
    J.R. DeLoach, K. Andrews, and C.L. Sheffield, Encapsulation of interleukin-2 in murine erythrocytes and subsequent deposition in mice receiving a subcutaneous injection. Biotechnol. Appl. Biochem. 10:183 (1988).PubMedGoogle Scholar
  10. 10.
    J.R. DeLoach and R.E. Droleskey, Survival of murine carrier erythrocytes injected via peritoneum. Comp. Biochem. Physiol. A 84:447 (1986).CrossRefGoogle Scholar
  11. 11.
    J.R. DeLoach and R.E. Droleskey, Preparation of ovine carrier erythrocytes: their action and survival. Comp. Biochem. Physiol. A 84:441 (1986).CrossRefGoogle Scholar
  12. 12.
    P.K. Gupta, Drug targeting in cancer chemotherapy: a clinical perspective. J. Pharm. Sci. 79:949 (1990).PubMedCrossRefGoogle Scholar
  13. 13.
    J.A. Jordán, R.B. Moyes, R. Droleskey, J.C. Diez, J. Luque, and J.R. DeLoach, In vitro and in vivo evaluation of BS3 and DTSSP crosslinked erythrocytes. Adv. Biosci. 92:17 (1994).Google Scholar
  14. 14.
    G. Lee, T.M. Delohery, Z. Ronai, P.W. Brandt-Rauf, M.R. Pincus, R.B. Murphy, and I.B. Weinstein, A comparison of techniques for introducing macromolecules into living cells. Cytometry 14:265 (1993).PubMedCrossRefGoogle Scholar
  15. 15.
    J. Luque, M.I. Garín, S. Sanz, P. Ropero, and M. Pinilla, Properties of hypotonized, crosslinked and crosslinked-permeabilized rat erythrocytes as potential carrier systems. Adv. Exp. Med. Biol. 326:81 (1992).PubMedGoogle Scholar
  16. 16.
    M. Magnani, G. Serafini, and V. Stocchi, Hexokinase type I multiplicity in human erythrocytes. Biochem. J. 254:617(1988).PubMedGoogle Scholar
  17. 17.
    M. Morrison, A.W. Michaels, D.R. Phillips, and S. Choi, Life span of erythrocyte membrane protein. Nature 248:763 (1974).PubMedCrossRefGoogle Scholar
  18. 18.
    M.T. Pérez, F.J. Alvarez, A.I. García-Pérez, L. Lucas, M.C. Tejedor, and P. Sancho, Heterogeneity of hypo-tonically loaded rat erythrocyte populations as detected by counter-current distribution in aqueus polymer two-phase systems. J. Chromatogr. B 677:45 (1996).Google Scholar
  19. 19.
    E. Pitt, C.M. Johnson, D.A. Lewis, D.A. Jenner, and R.E. Offord, Encapsulation of drugs in intact erythrocytes and intravenous delivery system. Biochem. Pharmacol. 32:3359 (1983).PubMedCrossRefGoogle Scholar
  20. 20.
    L. Rossi, M. Bianchi, A. Fraternale, and M. Magnani, Normalization of hyperglycemia in diabetic mice by enzyme-loaded erythrocytes. Adv. Exp. Med. Biol. 326:183 (1992).PubMedGoogle Scholar
  21. 21.
    M.C. Tejedor, C.E. Alvarez, F.J. Alvarez, A. Herráez, and J. Luque, Comparative encapsulation at different hypotonie pressures in human and rat erythrocytes. Adv. Biosci. 92:73 (1994).Google Scholar
  22. 22.
    M. Tonetti, C. Polvani, E. Zocchi, L. Guida, U. Benatti, P. Biassoni, F. Romei, A. Guglielmi, C. Aschete, A. Sobrero, and A. De Flora, Liver targeting of autologous erythrocytes loaded with doxorubicin. Eur. J. Cancer 27:947 (1991).PubMedCrossRefGoogle Scholar
  23. 23.
    E. Zocchi, L. Guida, U. Benatti, M. Canepa, L. Borgiani, T. Zanin, and A. De Flora, Hepatic or splenic targeting of carrier erythrocytes: a murine model. Biotechnol. Appl. Biochem. 9:423 (1987).PubMedGoogle Scholar
  24. 24.
    E. Zocchi, M Tonetti, C. Polvani, L. Guida, U. Benatti, and A. De Flora, In vivo liver and lung targeting of adriamycin encapsulated in glutaraldehyde-treated murine erythrocytes. Biotechnol. Appl. Biochem. 10:555(1988).PubMedGoogle Scholar
  25. 25.
    L. Zolla, G. Lupidi, M. Marcheggiani, G. Falcioni, and M. Brunori, Encapsulation of proteins into human erythrocytes: a kinetic investigation. Biochim. Biophys. Acta 1024:5 (1990).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • F. J. Alvarez
    • 1
  • J. A. Jordán
    • 1
  • J. C. Murciano
    • 1
  • J. Luque
    • 1
  • A. Herráez
    • 1
  • J. C. Díez
    • 1
  • M. C. Tejedor
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of AlcaláAlcalá de HenaresSpain

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