Biochemical Properties of Alcohol Dehydrogenase and Glutamate Dehydrogenase Encapsulated into Human Erythrocytes by a Hypotonic-Dialysis Procedure

  • Silvia Sanz
  • Carmen Lizano
  • Marina I. Garín
  • José Luque
  • Montserrat Pinilla


The stability against time (up to 170h) of encapsulated enzymes were studied in ADH- and GDH-carrier RBCs, at 4ºC and 37ºC, in comparison with that of free enzyme solutions. Encapsulation into RBCs suggest a protective effect of both enzyme activities. An efflux of the encapsulated enzymes from the carrier RBCs was observed during a similar incubation. The continuous degradation of ethanol and the simultaneous appearance/disappearance of acetaldehyde by ADH-RBCs, as a function of time (up to 72h), suggest the use of these carrier RBCs to fully metabolize ethanol. The rapid utilization of ammonia in the presence of GDH-RBCs suggest the use of these RBCs as carrier systems. These properties open the possibility of using ADH- and GDH-RBCs as carrier systems under in vivo situations.


Alcohol Dehydrogenase Glutamate Dehydrogenase Mean Cell Volume Human RBCs Index Human Blood 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Silvia Sanz
    • 1
  • Carmen Lizano
    • 1
  • Marina I. Garín
    • 1
  • José Luque
    • 1
  • Montserrat Pinilla
    • 1
  1. 1.Dpto. Bioquimica y Biol. Mol.Univ. AlcalaAlcala de Henares, MadridSpain

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