Biotinylation of Erythrocytes Prepares to Allow Circulation-Stable Immunoerythrocytes Capable of Recognizing the Antigen
Erythrocytes (RBC) have been studied as carriers of drugs for several years3. Their viability, physiologic characteristics and the availability of different procedures to incorporate substances into them support their utilization10. However, targeting is one of the major problems to solve in all these related therapies. Attachment of antibodies to the RBC surface has been pursued before2. This attachment may provide a way to target these carriers to the specific zone where the disease is, and also can serve as a means to eliminate hazardous antigens from the bloodstream. Biotin/streptavidin (b/SAv) complex is one of the systems to attach antibodies or other substances, such as enzymes, to the RBC surface5,9. Although there are several works which have proposed the development of immunoerythrocytes, little is known about their ability to recognize the antigen after their circulation in the animal model. Looking for these topics, in this work we investigate the attachment of biotinylated antibody (b-Ab) to biotinylated RBC by means of bridging SAv molecules. We show that moderate biotinylation of the RBC allows to attach 3 to 5 ×104 molecules of b-Ab and creates immunoerythrocytes that are stable in circulation. The b-Ab remains attached to the RBC while they are circulating. Furthermore, the immunoerythrocytes entirely retain the ability to recognize the antigen.
KeywordsLeft Axis Ester Lead Antigen Delivery System Antigen Binding Capacity Biotin Residue
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