Organophosphorus Antagonism by Resealed Erythrocytes Containing Recombinant Paraoxonase
Parathion has the highest incidence of poisonings among the agricultural pesticides. An alternative conceptual approach was employed to prevent intoxication of paraoxon, the active metabolite of parathion, by using resealed carrier erythrocytes containing a highly purified recombinant paraoxonase (CRBC). The CRBC were found to be effective alone and synergistic with 2-PAM and atropine to protect over 1,000 lethal doses of paraoxon. This striking protection greatly exceeds any antidotal regimen ever reported against chemical toxicants. The present drug carrier model provides a general conceptual approach to develop specific antidotes against many other chemical toxicants for which no antidotes are presently available.
KeywordsPotency Ratio Atropine Sulfate Carrier Cell Agricultural Pesticide Chemical Toxicant
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- 5.C. S. Steven, L. L. Harper, J. R. Wild, J. Bacteriol. 170, 2306 (1988). Synonyms: paraoxonase, organophosphorus acid anhydrase, parathion hydrolase, parathion arylesterase, and arylalkylphosphatase (EC 184.108.40.206).Google Scholar
- 7.R.J. Tallarida, R.B. Murray, Manual of Pharmacologie Calculations with Computer Program. (Springer-Verlag, New York ed. 2, 1987).Google Scholar
- L. Pei et al., Fundam. Appl. Toxicol. 124, 296 (1994).Google Scholar
- L. Pei et al., Biochem. Appl. Biotech., in press.Google Scholar
- 12.This study was supported by funds from Texas A&M University, USARMDC and NIEHS. We are grateful to Jennifer McNamee for her assistance in the preparation of this manuscript.Google Scholar