p53 Protein Expression in Thymic Epithelial Tumors

  • Naoki Hino
  • Kazuya Kondo
  • Takanori Miyoshi
  • Tadashi Uyama
  • Yasumasa Monden


We investigated the expression of p53 protein by immunohistochemical analysis using the anti-p53 polyclonal antibody (CM-1) in 12 thymomas and 13 thymic carcinomas.

All 6 noninvasive thymomas showed an absence of nuclear staining, and one of the 7 invasive thymomas (14%) showed nuclear staining focally. Eight of the 12 thymic carcinomas (67%) showed nuclear staining. Three of 8 cases showed nuclear staining in more than 50% of the tumor cells.

p53 protein accumulation is highly frequent in thymic carcinomas, but not in thymomas. The accumulation of p53 protein seems to play an important role in the carcinogenesis of thymic carcinomas.


Nuclear Staining Thymic Carcinoma Spindle Cell Carcinoma Thymic Epithelial Tumor Invasive Thymoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Kodama T, Watanabe S, Sato Y, Shimosato Y, Miyazawa N. An immunohistochemical study of thymic epithelial tumors I. Epithelial component. Am J Surg Pathol 1986; 10:26–33.PubMedCrossRefGoogle Scholar
  2. 2.
    Sato Y, Watanabe S, Mukai K, Kodama T, Upton MP, Goto M, et al. An immunohistochemical study of thymic epithelial tumors II. Lymphoid Component. Am J Surg Pathol 1986; 10:862–70.PubMedCrossRefGoogle Scholar
  3. 3.
    Kondo K, Mukai K, Sato Y, Matsuno Y, Shimosato Y, Monden Y. An immunohistochemical study of thymic epithelial tumors III. The distribution of interdigitating reticulum cells and S-100b-positive small lymphocytes. Am J Surg Pathol 1990; 14:1139–47.PubMedCrossRefGoogle Scholar
  4. 4.
    Bartek J, Bartkova J, Vojtesek B, Staskova Z, Lukas J, Rejthar A, et al. Aberrant expression of the p53 on-coprotein is a common feature of a wide spectrum of human malignancies. Oncogene 1991; 6:1699–703.PubMedGoogle Scholar
  5. 5.
    Masaoka A, Monden Y, Nakahara K, Tanioka T. Follow-up study of thymomas with special reference to their clinical stages. Cancer 1981; 48:2485–92.PubMedCrossRefGoogle Scholar
  6. 6.
    Kondo K, Umemoto A, Akimoto S, Uyama T, Hayashi K, Ohnishi Y, et al. Mutations in the P53 tumour suppressor gene in primary lung cancer in Japan. Biochem Biophys Res Commun 1992; 183:1139–46.PubMedCrossRefGoogle Scholar
  7. 7.
    Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991; 39:741–8.PubMedCrossRefGoogle Scholar
  8. 8.
    Shimosato Y, Kameya T, Nagai K, Suemasu K. Squamous cell carcinoma of the thymus. An analysis of eight cases. Am J Surg Pathol 1977; 1:109–21.PubMedCrossRefGoogle Scholar
  9. 9.
    Wick MR, Scheithauer BW, Weiland LH, Bernatz PE. Primary thymic carcinomas. Am J Surg Pathol 1982; 6:613–30.PubMedCrossRefGoogle Scholar
  10. 10.
    Truong LD, Mody DR, Cagle PT, Jackson YG, Schwartz MR, Wheeler TM. Thymic carcinoma. A clinicopathologic study of 13 cases. Am J Surg Pathol 1990; 14:151–66.PubMedCrossRefGoogle Scholar
  11. 11.
    Suster S, Rosai J. Thymic carcinoma. A clinicopathologic study of 60 cases. Cancer 1991; 67:1025–32.PubMedCrossRefGoogle Scholar
  12. 12.
    Shimosato Y. Controversies surrounding the subclassification of thymoma. Cancer 1994; 74:542–44.PubMedCrossRefGoogle Scholar
  13. 13.
    Hayashi K. PCR-SSCP: a method for detection of mutations. Genet Anal Tech Appl 1992; 9:73–9.PubMedCrossRefGoogle Scholar
  14. 14.
    Kirchner T, Schalke B, Buchwald J, Ritter M, Marx A, Müller HH. Well-differentiated thymic carcinoma. An organotypical low-grade carcinoma with relationship to cortical thymoma. Am J Surg Pathol 1992; 16:1153–69.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Naoki Hino
    • 1
  • Kazuya Kondo
    • 1
  • Takanori Miyoshi
    • 1
  • Tadashi Uyama
    • 1
  • Yasumasa Monden
    • 1
  1. 1.Second Department of Surgery, School of MedicineUniversity of TokushimaTokushimaJapan

Personalised recommendations