Preliminary Field Denitrification Studies of Nitrate-Fertilized and Nitrogen-Fixing Crops

  • B. H. Svensson
  • L. Klemedtsson
  • T. Rosswall
Part of the NATO Conference Series book series (NATOCS, volume 9)


Soil cores were sampled and treated by the acetylene inhibition technique to follow denitrification rates in fields with barley (0 and 120 kg N ha−1), meadow fescue grass ley (200 kg N ha−1) and lucerne. The acetylene inhibition method was also used in studies of denitrification potential.

Denitrification rates, elevated over a baseline rate, were obtained when the soil was wetted by rain. This was noted especially in the fertilized crops. Higher rates were obtained with samples taken in plant rows as compared with those between plant rows.The rates seemed to be highest during the autumn. A large variation between the cores may be due to an uneven distribution of denitrification potential in the soil. Potential denitrification rates were highest in the plough layers (down to about 30 cm), compared with the sand and clay layer underneath. The potential was also higher in the fertilized treatments than in those receiving no fertilizer nitrate. These results probably reflect energy availability and nitrate abundance, respectively.


Clay Layer Denitrification Rate Potential Denitrification Anaerobic Incubation Plough Layer 
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  1. Berg, P., Klemedtsson, L., and Rosswall, T., 1982, Inhibitory effect of low partial pressures of acetylene on nitrification, Soil Biol. Biochem., 14: 301.CrossRefGoogle Scholar
  2. Klemedtsson, L., 1981, Analysis of N20-production from soil systems, in: “Proceedings of the 2nd Scandinavian Head Space Meeting in Kongsberg (Norway), 12–14 Oct. 1980”, N. Berg, ed., Sentralinstitutt for Industriell Forskning, Oslo. ( In Swedish ).Google Scholar
  3. Klemedtsson, L., Svensson, B.H., Lindberg, T., and Rosswall, T., 1977, The use of acetylene inhibition of nitrous oxide reductase in quantifying denitrification in soils, Swedish J. Agric. Res., 7: 179.Google Scholar
  4. Klemedtsson, L., Berg, P., Rosswall, T., and Svensson, B.H., 1982, A method for the separation of N20 production from denitrification and nitrification, Abstr. 82nd Ann. Meet. ASM Athens, Georgia, (N 39 ): 184.Google Scholar
  5. Klemedtsson, L., Rosswall, T., and Svensson, B.H., in manuscript, Effects of actively growing roots on the denitrification and nitrification processes in soil.Google Scholar
  6. Rolston, D.E., Sharpley, A.N., Toy, D.W., and Broadbent, F.E., 1982, Field measurements of denitrification: III. Rates during irrigation cycles, Soil Sci. Soc. Am. J., 46: 289.Google Scholar
  7. Rosswall, T., 1977, Exchange of nutrients between atmosphere and vegetation, Agro-Ecosystems, 4: 296.Google Scholar
  8. Rosswall, T., Lohm, U., and Sohlenius, B., 1977, Développement d’un microcosme pour l’étude de la minéralisation et de l’absorption radiculaire de l’azote dans l’humus d’une forêt de conifères (Pinus sylvestris L.), Lejeunia N.S., 84: 1.Google Scholar
  9. Ryden, R.C., Lund, L.J., Letey, J., and Focht, D.D., 1979, Direct measurements of denitrification losses from soils. II. Development and application of field methods, Soil Sci. Soc. Am. J., 43: 110.Google Scholar
  10. Smith, M.S., and Tiedje, J.M., 1979. Phases of denitrification following oxygen depletion in soil, Soil Biol. Biochem., 11: 261.CrossRefGoogle Scholar
  11. Steen, E., Jansson, P.E., and Persson, J., 1984, Experimental site of the “Ecology of Arable Land” project, Acta Agric. Scand., 34: 153.CrossRefGoogle Scholar
  12. Stefansson, R.C., 1972a, Soil denitrification in sealed soil-plant systems. I. Effects of plants, soil water content and soil organic matter content, Plant and Soil, 33: 113.CrossRefGoogle Scholar
  13. Stefansson, R.C., 1972b, Soil denitrification in sealed soil plant systems, II. Effect of soil water content and applied nitrogen, Plant and Soil, 33: 129.Google Scholar
  14. Stefansson, R.C., 1972c, Soil denitrification in sealed soil-plant systems. III. Effect of distributed and undistributed soil samples, Plant and Soil, 33: 141.CrossRefGoogle Scholar
  15. Yoshinari, T., and Knowles, R., 1976, Acetylene inhibition of nitrous oxide reduction of denitrifying bacteria, Biochem. Biophys. Res. Comm., 69: 705.Google Scholar
  16. Yoshinari, T., Hynes, R., and Knowles, R., 1977, Acetylene inhibition of nitrous oxide reduction and measurement of denitrification and nitrogen fixation in soil, Soil Biol. Biochem., 9: 177.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • B. H. Svensson
    • 1
  • L. Klemedtsson
    • 1
  • T. Rosswall
    • 2
  1. 1.Department of MicrobiologySwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Department of Water in Environment and SocietyUniversity of LinköpingLinköpingSweden

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