Dendritic Cells and Interleukin 12 as Adjuvants for Tumor-Specific Vaccines

  • Ursula Grohmann
  • Roberta Bianchi
  • Maria Laura Belladonna
  • Silvia Silla
  • Daniela Surace
  • Maria Cristina Fioretti
  • Paolo Puccetti
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

The demonstration of autologous CD8 cytotoxic T lymphocytes (CTL) with specificity for class I MHC-restricted tumor antigens is a cornerstone in the field of tumor immunology and immunotherapy, raising the critical issue of developing effective means of increasing the frequency of tumor-specific CTL and translating a trace repertoire into effective immune responses1. Dendritic cells (DC) are unmatched in their ability to present antigen to naive T cells in a way that activates the immune response2. Recent work in our laboratory has begun to address this challenge by using DC to elicit antigen-specific CD8 cell responses in vivo in mice3–5. In particular, our model system makes use of DC pulsed in vitro with a well-characterized peptide related to the tumor rejection antigen P815AB6 of mastocytoma P815, and the tumor peptide-loaded DC are transferred into recipient mice, that are monitored for development of CD8 cell reactivity by a skin test assay.

Keywords

Dendritic Cell Skin Test Reactivity Tumor Rejection Antigen Helper Epitope Anergic State 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Ursula Grohmann
    • 1
  • Roberta Bianchi
    • 1
  • Maria Laura Belladonna
    • 1
  • Silvia Silla
    • 1
  • Daniela Surace
    • 1
  • Maria Cristina Fioretti
    • 1
  • Paolo Puccetti
    • 2
  1. 1.Department of Experimental MedicineUniversity of PerugiaPerugiaItaly
  2. 2.University of Tor VergataRomeItaly

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