Identification and Isolation of CD1a Positive Putative Tumour Infiltrating Dendritic Cells in Human Breast Cancer

  • Brendon J. Coventry
  • Jonathan M. Austyn
  • Steve Chryssidis
  • Debra Hankins
  • Adrian Harris
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)


Identification of dendritic cells (DC) in human tissues has been technically problematic due to the lack of truly specific immunohistochemical markers for DC’s. Human dendritic cells express CD la glycoprotein at certain points in their life cycle. CD1a positive cells are present in many human tumours and have been associated with improved survival. However, little information exists concerning the separation of DC from human tumours. The current study reports that human breast carcinomas have low densities of CD1a positive cells with dendritic morphology, and details are shown of a technique for successful separation of these cells from tumour tissues.


Dendritic Cell Human Breast Carcinoma Ductal Breast Carcinoma Current Study Report Royal Australasian College 
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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Brendon J. Coventry
    • 1
  • Jonathan M. Austyn
    • 2
  • Steve Chryssidis
    • 1
  • Debra Hankins
    • 2
  • Adrian Harris
    • 3
  1. 1.Department of SurgeryUniversity of Adelaide Royal Adelaide HospitalNorth Terrace, AdelaideAustralia
  2. 2.Nuffield Department of SurgeryUniversity of OxfordHeadington, OxfordUK
  3. 3.Department of Clinical OncologyUniversity of OxfordHeadington, OxfordUK

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